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You searched for: Extraction Buffers

Extraction Buffers

Extraction buffers are provided ready to use and are effective in the isolation of total RNA and other proteins, improving protein yields and sample complexity from cells and tissues. Optimized extraction reagents enhance protein extraction in native or denaturing buffers compatible with downstream analytical techniques. An appropriate buffer solution added to a protein mixture during the extraction process can help improve the stability of protein molecules as these molecules are subjected to various forces designed to isolate them for study.

J.T.Baker® Cell Lysis Solution, Ultrapure Bioreagent

J.T.Baker® Cell Lysis Solution, Ultrapure Bioreagent

Supplier: AVANTOR PERFORMANCE MATERIAL LLC

J.T.Baker® Cell Lysis Solution. Optimized multi-component, ready-to-use solution for extraction and recovery of AAV particles from HEK293 or SF9 cells. The product offers quantitative recovery through efficient cell lysis and protection of vector particles against shear stress. The solution is formulated with non-ionic detergents which are biodegradable and octyl phenol ethoxylate free.

    
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J.T.Baker® Cell Lysis Solution, Biotech Reagent

J.T.Baker® Cell Lysis Solution, Biotech Reagent

Supplier: AVANTOR PERFORMANCE MATERIAL LLC

J.T.Baker® Cell Lysis Solution Biotech Reagent is a cGMP pre-formulated and ready to use detergent optimized for the extraction and recovery of AAV particles from producer cell lines (HEK293 parental and suspension lines, SF9). J.T.Baker® Cell Lysis Solution is a proprietary blend of nonionic surfactants, free of octyl phenol ethoxylate, which demonstrates comparable performance industry standard detergents while addressing environmental concerns associated with REACH and downstream clearance concerns. Designed for Gene Therapy workflows, this formulation is easy to use and handle as it is ready-to-use, has low viscosity, does not require cold temperature shipping or storage, and is available in sizes to support processes from development to large scale commercial batches. Detail description provided in technical literature.

    
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RIPA Lysis and Extraction Buffer, G-Biosciences

Supplier: G-Biosciences

G-Biosciences' RIPA Lysis and Extraction Buffer is a complete lysis buffer for the release of cytoplasmic, membrane and nuclear proteins from adherent and suspension cultured mammalian cells

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PhosphoSafe™ Extraction Reagent, MilliporeSigma

Supplier: MilliporeSigma

PhosphoSafe™ extraction buffer efficiently extracts cytosolic proteins from mammalian and insect cells while preserving their phosphorylation state.

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FOCUS™ Extraction Buffers, G-Biosciences

FOCUS™ Extraction Buffers, G-Biosciences

Supplier: G-Biosciences

One of the most important considerations before running 2D gel electrophoresis is the choice of protein solubilization buffers

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Extraction Reagents 1 (5X), (10 ml), Enzo Life Sciences

Supplier: Enzo Life Sciences

Concentrated lysis buffer for preparation of cell and tissue extracts.

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Extraction Reagents 2 (5X), (10 ml), Enzo Life Sciences

Supplier: Enzo Life Sciences

Concentrated lysis buffer for preparation of cell and tissue extracts.

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Buffers for E.Z.N.A.® Gel Extraction Systems, Omega Bio-tek®

Supplier: Omega Bio-Tek

Binding Buffer (XP2), Omega Bio-Tek

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Protein Extraction and Lysis Buffer (PE LB™) Systems, G-Biosciences

Protein Extraction and Lysis Buffer (PE LB™) Systems, G-Biosciences

Supplier: G-Biosciences

Lysis and extraction of biologically active proteins from cellular and tissue samples is the first critical step for biochemical analysis

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DNA Pre-wash Buffer, Zymo Research

DNA Pre-wash Buffer, Zymo Research

Supplier: Zymo Research

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Monarch® gDNA Nuclei Prep and Lysis Buffer Pack, New England Biolabs

Monarch® gDNA Nuclei Prep and Lysis Buffer Pack, New England Biolabs

Supplier: New England Biolabs (NEB)

The Monarch® gDNA Nuclei Prep and lysis buffer pack contains two lysis buffers used in the Monarch HMW DNA extraction kit for cells and blood.

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RNA Binding Buffer, Zymo Research

RNA Binding Buffer, Zymo Research

Supplier: Zymo Research

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RNA Lysis Buffer, Zymo Research

RNA Lysis Buffer, Zymo Research

Supplier: Zymo Research

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ACK Lysing Buffer, Quality Biological

ACK Lysing Buffer, Quality Biological

Supplier: QUALITY BIOLOGICAL, INC.

Ammonium-Chloride-Potassium (ACK) Lysing Buffer is used for the lysis of red blood cells in samples containing white blood cells, such as EDTA-treated whole blood, buffy coats, and bone marrow.

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Sodium Dodecyl Sulfate SDS, 20%

Supplier: Rockland Immunochemical

SDS, sodium dodecyl sulfate is an anionic surfactant.

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Pierce™ RIPA Buffer, Thermo Scientific

Pierce™ RIPA Buffer, Thermo Scientific

Supplier: Thermo Scientific

Thermo Scientific RIPA Buffer is a high-quality, ready-to-use and fully disclosed formulation of a popular cell lysis reagent for cultured mammalian cells.

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Monarch® gDNA Elution Buffer II, New England Biolabs

Monarch® gDNA Elution Buffer II, New England Biolabs

Supplier: New England Biolabs (NEB)

The Monarch® gDNA elution buffer II is supplied as a component of the Monarch HMW DNA extraction kits.

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Y-Lysis Buffer, Zymo Research

Y-Lysis Buffer, Zymo Research

Supplier: Zymo Research

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ZR RNA Buffer, Zymo Research

ZR RNA Buffer, Zymo Research

Supplier: Zymo Research

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RNA Pre-Wash Buffer, Zymo Research

RNA Pre-Wash Buffer, Zymo Research

Supplier: Zymo Research

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Solution 3 Neutralizing Buffer, Zymo Research
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Cell lysis buffer 4, (100 ml), Enzo Life Sciences

Supplier: Enzo Life Sciences

Cell lysis buffer 4, (100 ml), Enzo Life Sciences

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SideStep II Cell Lysis Analysis Kit, Agilent Technologies

SideStep II Cell Lysis Analysis Kit, Agilent Technologies

Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA

Progress from mammalian cells to qPCR or QRT-PCR experiments without nucleic acid purification.

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Lysis Buffer, FLEXLyse

Supplier: Beckman Coulter

Whole blood assays are our best approximation of physiological conditions in research flow cytometry. Density gradient leukocyte isolation or bulk lysing procedures are common to remove red blood cells, but decompose the native material prior to antigen detection. Common lysing buffers avoid this decomposition and eliminate the gradient-related preanalytical workload, but also increase sample volume beyond the single-well capacity of many plate formats, limiting throughput and automation options.

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Pierce™ DNA Extraction Buffer, Phenol:Chloroform and Tris Buffer, Thermo Scientific

Pierce™ DNA Extraction Buffer, Phenol:Chloroform and Tris Buffer, Thermo Scientific

Supplier: Thermo Scientific

Thermo Scientific Pierce Phenol-Chloroform is phenol supplied premixed with chloroform:isoamyl alcohol (25:24:1).

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g-DNA Wash Buffer, Zymo Research

g-DNA Wash Buffer, Zymo Research

Supplier: Zymo Research

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Lysis Buffer 7X (Blue), Zymo Research
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Solution 2 Lysis Buffer, Zymo Research
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ZymoBIOMICS Lysis Solution, Zymo Research

ZymoBIOMICS Lysis Solution, Zymo Research

Supplier: Zymo Research

ZymoBIOMICS Lysis Solution is designed to be used with the ZymoBIOMICS DNA extraction kits. Used in combination with the ZR BashingBead Lysis Tubes, it assists with microbial lysis. It is certified to be low-bioburden to ensure minimal background contaminants for low abundance microbiome samples.

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