Blocking Buffers

Supplier: VWR

Solution reduces blocking time to 5 minutes for Western and dot blotting procedures on PVDF and nitrocellulose membranes.

Supplier: Azure Biosystems

Azure Chemi Blot Blocking Buffer, Ready-to-use block that enhances chemiluminescent signal, for use with chemiluminescent Western blots to enhance signal and lower background. Size: 500mL

Supplier: Abcam

10X blocking buffer (pH 7.5) for serum-free blocking of non-specific antibody binding. - Cited in over 30 publications - Suitable for ELISA, in-cell ELISA, ICC/IF, western blot (WB) - 100% casein blocking as preservative

Supplier: Thermo Scientific Chemicals

Viscous liquid

Supplier: Thermo Scientific

SuperBlock™ Blocking Buffer is an optimized solution containing a single purified glycoprotein that provides incredibly fast and effective blocking for ELISA, IHC, and western blot analysis.

Supplier: Thermo Scientific

Thermo Scientific Fish Serum Blocking Buffer is steelhead salmon serum in PBS that is especially useful as a blocking agent in IHC and other detection methods involving mammalian samples.

Supplier: Thermo Scientific

Blocker BSA in PBS or TBS are ready-to-use, 1X PBS or TBS solutions of 10X (10%) bovine serum albumin protein for blocking steps in Western blot, ELISA, IHC and nucleic acid detection methods.

Supplier: Thermo Scientific

ELISA Blocker Blocking Buffer is a protein buffer that effectively blocks plate binding sites that remain in polystyrene microplate wells following initial coating steps in ELISA procedures. The ready-to-use buffer is optimized and routinely used for sandwich ELISA procedures involving cytokines, such as many of the Thermo Scientific Pierce ELISA Kits.

Supplier: Thermo Scientific

Thermo Scientific SuperBlock™ Blocking Buffer is optimised solutions in PBS or TBS containing a protein that provides incredibly fast and effective blocking for ELISA, IHC and Western blot analysis.

Supplier: Thermo Scientific

Thermo Scientific Pierce Clear Milk Blocking Buffer (10X) is a long shelf-life, high performance replacement for homemade milk blocking buffers in Western blotting applications.

Supplier: Thermo Scientific

This Fish Serum Blocking Buffer is Steelhead Salmon Serum in PBS that is especially useful as a blocking agent in IHC and other detection methods involving mammalian samples.

Supplier: QUALITY BIOLOGICAL, INC.

Blocking is a very important step of western blotting, as it prevents antibodies from binding to the membrane nonspecifically. Blocking is often made with 5% BSA or nonfat dried milk to reduce the background. Nonfat dried milk is often preferred as it is inexpensive and widely available. However, milk proteins are not compatible with all detection labels, so care must be taken to choose the appropriate blocking solution.

Supplier: Abcam

Immunoassay Blocking (BSA Free) (ab171535) maintains the activity of antibodies and other biomolecules adsorbed or immobilized to solid substrates.

Supplier: Thermo Scientific

SuperBlock (TBS) Blocking Buffer is an optimised TBS solution containing a protein that provides incredibly fast and effective blocking for ELISA, IHC and Western Blot Analysis.

Supplier: Thermo Scientific

Thermo Scientific Pierce Fast Blocking Buffer effectively and reliably blocks Western blots in just five minutes to provide low-background results and speed up traditional blotting protocols.

Supplier: Thermo Scientific

These protein-free blocking buffers are either a PBS or a TBS formulation of a non protein compound that provides effective blocking for membrane based and plate based protein detection methods, resulting in extremely low background.