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Cloning Buffers

In molecular biology, cloning buffer solutions containing TRIS, acetic acid, EDTA, or more are used in agarose electrophoresis, typically for the separation of nucleic acids such as DNA and RNA. Various buffer formulations are available with different base ingredients in the mixture. These different mixtures have varied buffer capacities and run times. Buffer solutions serve to protect samples against pH changes while the sample is undergoing a wide variety of chemical applications.

EDTA, Standard Solution, 0.5M, VWR Chemicals BDH®

EDTA, Standard Solution, 0.5M, VWR Chemicals BDH®

Supplier: VWR International

N.I.S.T traceable to SRM 919.

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VWR® TRIS EDTA Buffer, Biotechnology Grade
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VWR® EDTA 0.5M, Biotechnology Grade

VWR® EDTA 0.5M, Biotechnology Grade

Supplier: VWR

Cloning Buffer, EDTA, 0.5M Solution, pH 8.0

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VWR® EDTA 0.5M, Biotechnology Grade

VWR® EDTA 0.5M, Biotechnology Grade

Supplier: VWR

Cloning Buffer, EDTA, 0.5M Solution, pH 8.0

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VWR® Sodium Acetate, 3M Solution, pH 5.2, Biotechnology Grade

VWR® Sodium Acetate, 3M Solution, pH 5.2, Biotechnology Grade

Supplier: VWR

Cloning Buffer, Sodium acetate solution 3M, pH 5.2

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TNE Buffer, Quality Biological

TNE Buffer, Quality Biological

Supplier: QUALITY BIOLOGICAL, INC.

TNE Buffer is often used as a base material to generate lysis buffers.

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1M Tris, pH 7.5

Supplier: GROWCELLS

Tris (tris[hydroxymethyl]aminoethane) is widely used as a component of buffer solutions

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TE (Tris EDTA) Buffers, Quality Biological

TE (Tris EDTA) Buffers, Quality Biological

Supplier: QUALITY BIOLOGICAL, INC.

TE is composed of buffering agent Tris, and chelating agent EDTA. EDTA prevents degradation of DNA and RNA by chelating magnesium (or other divalent metal ions). Together, this Tris-EDTA based solution solubilizes DNA or RNA, while protecting it from degradation.

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Low-EDTA TE (1X), pH 8.0, Quality Biological

Low-EDTA TE (1X), pH 8.0, Quality Biological

Supplier: QUALITY BIOLOGICAL, INC.

Low-EDTA 1X, pH 8.0 is molecular biology grade TE buffer used to store DNA and RNA. EDTA chelates Mg2+ and other divalent metals ions - (inhibits DNAse and RNAse to suppress DNA and RNA degradation).

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TE (Tris EDTA), 1X, pH 8.0 (Nuclease Free)

Supplier: GROWCELLS

Nuclease free TE is used to solubilize DNA/RNA while providing protection from degradation

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EDTA (0.5 M), pH 8.0, Quality Biological

EDTA (0.5 M), pH 8.0, Quality Biological

Supplier: QUALITY BIOLOGICAL, INC.

EDTA (Ethylenediaminetetraacetic acid), a metal chelator, is widely used in molecular biology as a chemical reagent to reduce nuclease activity (i.e. metalloenzyme). EDTA solution can be used to study cell biology, molecular biology, bioactive small molecules, biochemicals, solutions and reagents, inorganic salts, and chelators.

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ReadiUse™ Universal Kinase Reaction Stopping Buffer

ReadiUse™ Universal Kinase Reaction Stopping Buffer

Supplier: AAT BIOQUEST INC

ReadiUse™ Universal Kinase Reaction Stopping Buffer is an aqueous solution that effectively chelates divalent metal ions.

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DNase I Reaction Buffer, New England Biolabs

DNase I Reaction Buffer, New England Biolabs

Supplier: New England Biolabs (NEB)

New England Biolabs supplies a 10X reaction buffer with all of its enzymes

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Thrombin Buffer, pH 8.0, Thermo Scientific

Supplier: Thermo Scientific Chemicals

Liquid

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Exonuclease I Reaction Buffer, New England Biolabs

Exonuclease I Reaction Buffer, New England Biolabs

Supplier: New England Biolabs (NEB)

New England Biolabs supplies a 10X reaction buffer with all of its enzymes

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Buffer, EDTA Buffer Solution pH 8,5 (0,5 mol/L)

Supplier: G-Biosciences

A DNase and RNase free solution of 0.5M EDTA.

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DNA Suspension Buffer, Teknova

Supplier: Teknova

RNAse, DNAse Tested (10mM Tris, 0.1mM EDTA)

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T4 Polynucleotide Kinase Reaction Buffer, New England Biolabs

T4 Polynucleotide Kinase Reaction Buffer, New England Biolabs

Supplier: New England Biolabs (NEB)

New England Biolabs supplies a 10X reaction buffer with all of its enzymes

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T4 DNA Ligase Reaction Buffer, New England Biolabs

T4 DNA Ligase Reaction Buffer, New England Biolabs

Supplier: New England Biolabs (NEB)

New England Biolabs supplies a 10X reaction buffer with all of its enzymes

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Buffer, Sodium Acetate Buffer Solution pH 5,2 (3 mol/L), ROCK

Supplier: Rockland Immunochemical

3.0 M Sodium Acetate, pH 5.2

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Taq DNA Ligase Reaction Buffer, New England Biolabs

Taq DNA Ligase Reaction Buffer, New England Biolabs

Supplier: New England Biolabs (NEB)

New England Biolabs supplies a 10X reaction buffer with all of its enzymes

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Buffer, TE Solution (TRIS-EDTA Buffer) pH 8.0

Supplier: Rockland Immunochemical

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Antarctic Phosphatase Reaction Buffer, New England Biolabs

Antarctic Phosphatase Reaction Buffer, New England Biolabs

Supplier: New England Biolabs (NEB)

New England Biolabs supplies a 10X reaction buffer with all of its enzymes

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NEBNEXT® End Repair Reaction Buffer, New England Biolabs

NEBNEXT® End Repair Reaction Buffer, New England Biolabs

Supplier: New England Biolabs (NEB)

New England Biolabs supplies a 10X reaction buffer for use with NEBNext® End Repair Enzyme Mix

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AccuGENE® 1X TE Buffers

AccuGENE® 1X TE Buffers

Supplier: Lonza

AccuGENE™ Molecular Biology Buffers are ready-to-use solutions ideal for a wide range of molecular biology applications.

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Alkaline Phosphatase Buffer-1 (5X), Thermo Scientific

Supplier: Thermo Scientific Chemicals

Liquid

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Transformation buffer-2, pH 7.5, Thermo Scientific

Supplier: Thermo Scientific Chemicals

Liquid

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T4 RNA Ligase Reaction Buffer, New England Biolabs

T4 RNA Ligase Reaction Buffer, New England Biolabs

Supplier: New England Biolabs (NEB)

New England Biolabs supplies a 10X reaction buffer with all of its enzymes

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THERMOPOL® Reaction Buffer Pack , New England Biolabs

THERMOPOL® Reaction Buffer Pack , New England Biolabs

Supplier: New England Biolabs (NEB)

10X ThermoPol Reaction Buffer is optimized for use with VentR® and Deep VentR™ DNA Polymerases

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