Vectors, Plasmids and Libraries
In molecular cloning, vectors are DNA molecules used to artificially deliver foreign genetic material into another cell. The four major types of vectors are plasmids, viral vectors, cosmids, and artificial chromosomes, with the most commonly used of these being plasmids. Vectors may be used for cloning, and certain vectors are specifically tailored to that exact purpose, while others may be designed specifically for transcription and protein expression. Simpler transcription vectors are used to amplify their inserted genetic material.
Ward's® Plasmid DNA for Transformations
Supplier: Edvotek
These plasmids allow you to perform bacterial transformations, conferring new antibiotic resistance, as a visual way of demonstrating gene transfer.
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sparQ mRNA-Seq Kit
Supplier: Quantabio
Ultra-sensitive mRNA library prep workflow with efficient poly(A) capture.
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Polyadenylic acid (poly-A)
Supplier: Cytiva
Poly(A) (polyadenylic acid) can be used to evaluate the binding of potential therapeutic agents to mRNA in drug development research.
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pBR322 from Escherichia coli, MP Biomedicals
Supplier: MP Biomedicals
pBR322 is a 4363 bp vector purified from Escherichia coli.
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AAV Helper-Free System, Agilent Technologies
Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA
The AAV Helper-Free System eliminates the requirement for wild-type adenovirus co-infection from both AAV vector production and AAV stock titering steps, making this system entirely helper virus-free and safer. This system works with a broad range of hosts and infects both dividing and non-dividing cells.
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InterPlay Adenoviral TAP System, Agilent Technologies
Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA
The InterPlay Mammalian TAP system allows you to recover interacting proteins from mammalian cells.
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Pierce™ Gaussia-Dura Luc Vector for Luciferase Assays, Expression Vectors, Thermo Scientific
Supplier: Thermo Scientific
Gaussia Dura Luc vectors contain a mutant form of the Gaussia luciferase gene that confers better bioluminescent signal stability than native luciferase. Gaussia-Dura luciferase (approx. 20 kDa) is a secreted protein, which enables measurement of the reporter activity in media (for real-time assays) and in cell lysates.
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AdEasy Adenoviral Vector Systems, Agilent Technologies
Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA
The AdEasy system saves you a month of work over traditional methods by producing the recombinant adenoviral plasmid by homologous recombination in E. coli. The AdEasy XL system includes BJ5183 cells pre-transformed with the pAdEasy-1 plasmid, this feature dramatically decreases background caused by the non-recombinant shuttle plasmid.
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pBlueScript II Vectors, Agilent Technologies
Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA
The pBluescript II phagemids (plasmids with a phage origin) are cloning vectors designed to simplify commonly used cloning and sequencing procedures, including the construction of nested deletions for DNA sequencing, generation of RNA transcripts in vitro and site-specific mutagenesis and gene mapping.
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Vector, E. coli BL21, Cytiva
Supplier: Cytiva
The E. coli BL21 strain is protease-deficient for optimal expression of a variety of recombinant proteins.
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Pierce™ Cell-Free Protein Expression Vectors, T7, Mammalian, Thermo Scientific
Supplier: Thermo Scientific
Thermo Scientific T7 Cell-Free Expression Vectors (pT7CFE1) are cloning plasmids optimized to use with the 1-Step Human in vitro Protein Expression System for in vitro translation (IVT) of tagged fusion proteins.
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SureVector, Vectors, Agilent Technologies
Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA
Combine standard DNA components to build custom vector constructs from Agilent’s set of standard parts.
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InterPlay Adenoviral TAP Systems, Agilent Technologies
Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA
The InterPlay Adenoviral TAP System combines our unique tandem affinity purification (TAP) system with our exclusive adenoviral gene delivery system, the AdEasy Adenoviral Vector System, for enhanced gene delivery to a broader range of mammalian cells, higher protein yields, and improved purification and analysis of endogenous interacting protein partners.
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AdEasy Adenoviral Vector Systems, Agilent Technologies
Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA
The AAV helper-free system eliminates the requirement for wild-type adenovirus co-infection from both AAV vector production and AAV stock titering steps, making this system entirely helper virus-free and safer.
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Expression vectors, pGEX
Supplier: Cytiva
The pGEX vectors have an expanded multiple cloning site (MCS) that contains six restriction sites. The expanded MCS facilitates the unidirectional cloning of cDNA inserts obtained from libraries constructed using many available lambda vectors. pGEX-6P-1, pGEX-6P-2, and pGEX-6P-3 each encode the recognition sequence for site-specific cleavage by PreScission Protease between the GST domain and the multiple cloning site. pGEX-4T-1, pGEX-4T-2, and pGEX-4T-3 are derived from pGEX-2T and contain a Thrombin recognition site. pGEX-5X-1, pGEX-5X-2, and pGEX5X-3 are derivatives of pGEX-3X and possess a Factor Xa recognition site.
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Pierce™ Green Renilla Luc Vector for Luciferase Assays, Expression Vectors, Thermo Scientific
Supplier: Thermo Scientific
Green Renilla Luc vectors contain a gene cloned from the sea pansy, Renilla reniformis
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Catalog #: CA21905-026
Supplier: Kimberly-Clark