You searched for: Vectors, Plasmids and Libraries
In molecular cloning, vectors are DNA molecules used to artificially deliver foreign genetic material into another cell. The four major types of vectors are plasmids, viral vectors, cosmids, and artificial chromosomes, with the most commonly used of these being plasmids. Vectors may be used for cloning, and certain vectors are specifically tailored to that exact purpose, while others may be designed specifically for transcription and protein expression. Simpler transcription vectors are used to amplify their inserted genetic material.
Ward's® Plasmid DNA for Transformations
Supplier: Avantor
These plasmids allow you to perform bacterial transformations, conferring new antibiotic resistance, as a visual way of demonstrating gene transfer.
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Polyadenylic acid (poly-A)
Supplier: Cytiva
Poly(A) (polyadenylic acid) can be used to evaluate the binding of potential therapeutic agents to mRNA in drug development research.
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InterPlay Adenoviral TAP System, Agilent Technologies
Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA
The InterPlay Mammalian TAP system allows you to recover interacting proteins from mammalian cells.
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AAV Helper-Free System, Agilent Technologies
Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA
The AAV Helper-Free System eliminates the requirement for wild-type adenovirus co-infection from both AAV vector production and AAV stock titering steps, making this system entirely helper virus-free and safer. This system works with a broad range of hosts and infects both dividing and non-dividing cells.
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InterPlay Adenoviral TAP Systems, Agilent Technologies
Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA
The InterPlay Adenoviral TAP System combines our unique tandem affinity purification (TAP) system with our exclusive adenoviral gene delivery system, the AdEasy Adenoviral Vector System, for enhanced gene delivery to a broader range of mammalian cells, higher protein yields, and improved purification and analysis of endogenous interacting protein partners.
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SureVector, Vectors, Agilent Technologies
Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA
Combine standard DNA components to build custom vector constructs from Agilent’s set of standard parts.
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AdEasy Adenoviral Vector Systems, Agilent Technologies
Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA
The AdEasy system saves you a month of work over traditional methods by producing the recombinant adenoviral plasmid by homologous recombination in E. coli. The AdEasy XL system includes BJ5183 cells pre-transformed with the pAdEasy-1 plasmid, this feature dramatically decreases background caused by the non-recombinant shuttle plasmid.
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pBlueScript II Vectors, Agilent Technologies
Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA
The pBluescript II phagemids (plasmids with a phage origin) are cloning vectors designed to simplify commonly used cloning and sequencing procedures, including the construction of nested deletions for DNA sequencing, generation of RNA transcripts in vitro and site-specific mutagenesis and gene mapping.
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Vector, E. coli BL21, Cytiva
Supplier: Cytiva
The E. coli BL21 strain is protease-deficient for optimal expression of a variety of recombinant proteins.
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Expression vectors, pGEX
Supplier: Cytiva
The pGEX vectors have an expanded multiple cloning site (MCS) that contains six restriction sites. The expanded MCS facilitates the unidirectional cloning of cDNA inserts obtained from libraries constructed using many available lambda vectors. pGEX-6P-1, pGEX-6P-2, and pGEX-6P-3 each encode the recognition sequence for site-specific cleavage by PreScission Protease between the GST domain and the multiple cloning site. pGEX-4T-1, pGEX-4T-2, and pGEX-4T-3 are derived from pGEX-2T and contain a Thrombin recognition site. pGEX-5X-1, pGEX-5X-2, and pGEX5X-3 are derivatives of pGEX-3X and possess a Factor Xa recognition site.
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AdEasy Adenoviral Vector Systems, Agilent Technologies
Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA
The AAV helper-free system eliminates the requirement for wild-type adenovirus co-infection from both AAV vector production and AAV stock titering steps, making this system entirely helper virus-free and safer.
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pJY2 Plasmid, Enzo Life Sciences
Supplier: Enzo Life Sciences
pJY2 encodes tRNAUCUArg to suppress lysine misincorporation at AGA codons and T7 lysozyme to depress constitutive expression of the cloned gene. pJY2 can also be used as a source of tRNAUCUArg with expression systems, such as the pGEX series of vectors, that do not rely on T7 polymerase.
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pBR322 from Escherichia coli, MP Biomedicals
Supplier: MP Biomedicals
pBR322 is a 4363 bp vector purified from Escherichia coli.
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VSV-Pseudovirus_SARS-CoV-2 Delta Luciferase, ReVacc Scientific
Supplier: ReVacc Scientific
This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the S protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations initially identified in variant of B.1.617.2 (Delta strain), and 18-aa cytoplasmic tail truncation for optimal infection.
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VSV-Pseudovirus_SARS-CoV-2 Omicron BA.2.75 Strain Spike with Luciferase Reporter
Supplier: ReVacc Scientific
This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the S protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations initially identified in variant of Omicron BA.2.75. The S has 18-aa cytoplasmic tail truncation for optimal infection.
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VSV-Pseudotyped HMPV Fusion with Luciferase Reporter (NL/1/00/A1 strain)
Supplier: ReVacc Scientific
This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the full length fusion (F) protein of Human metapneumovirus (HMPV) isolate NL/1/00/A1 (294E). HMPV is a respiratory virus with fusion (F) protein as the sole target of neutralizing antibodies. As pseudovirus infectivity of rVSV without its original G is restricted to a single round of replication, the pseudotypes can be handled using BSL-2 containment practices.
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M13mp18 Single-Stranded DNA, New England Biolabs
Supplier: New England Biolabs (NEB)
The single-stranded viral DNA is isolated from M13mp18
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pSNAPf Vector, New England Biolabs
Supplier: New England Biolabs (NEB)
pSNAPf Vector is a mammalian expression plasmid intended for the cloning and stable or transient expression of SNAP-tag® protein fusions in mammalian cells
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VSV-Pseudovirus_SARS-CoV-2 Omicron BA.2.86 Luciferase
Supplier: ReVacc Scientific
This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the S protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations initially identified in variant of Omicron BA. The S has 18-aa cytoplasmic tail truncation for optimal infection.
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Ph.D.-12 Phage Display Peptide Library Kit v2, New England Biolabs
Supplier: New England Biolabs (NEB)
The Ph.D.−12 Phage display peptide library kit v2 is based on a combinatorial library of random dodecapeptides fused to the N-terminus of a minor coat protein (pIII) of M13 phage. The peptide is followed by a short spacer (Gly-Gly-Gly) which directly precedes the wild-type pIII sequence. The library consists of ~1E09 electroporated sequences amplified once to yield approximately 100 copies of each sequence in 10 µl of the supplied phage.
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VSV-Pseudovirus_SARS-CoV-2 Omicron BQ.1.1 Strain Spike with Luciferase Reporter
Supplier: ReVacc Scientific
This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the S protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations initially identified in variant of Omicron BQ.1.1. The S has 18-aa cytoplasmic tail truncation for optimal infection.
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Pierce™ Gaussia-Dura Luc Vector for Luciferase Assays, Expression Vectors, Thermo Scientific
Supplier: Thermo Scientific
Gaussia Dura Luc vectors contain a mutant form of the Gaussia luciferase gene that confers better bioluminescent signal stability than native luciferase. Gaussia-Dura luciferase (approx. 20 kDa) is a secreted protein, which enables measurement of the reporter activity in media (for real-time assays) and in cell lysates.
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VSV-Pseudovirus_SARS-CoV-2 Omicron XBB.1.5 Strain Spike with Luciferase Reporter
Supplier: ReVacc Scientific
This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the S protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations initially identified in variant of Omicron XBB.1.5. The S has 18-aa cytoplasmic tail truncation for optimal infection.
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Lentivirus Pseudotyped Influenza A HPAI H5N1 virus HaNaM with Luciferase Reporter (clade 2.3.4.4b, 2022 strain)
Supplier: ReVacc Scientific
This pseudotyped virus uses lentivirus packaging to carry full length HA, NA and M of Highly Pathogenic Avian Influenza (HPAI) A virus (A/Pelecanus/Peru/VFAR-140/2022(H5N1), clade 2.3.4.4b) (GenBank: OQ565628.1). The pseudotypes can be handled using BSL-2 containment practices.
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PH.D.-7 Phage Display Peptide Library Kit v2, New England Biolabs
Supplier: New England Biolabs (NEB)
The Ph.D.−7 phage display peptide library kit v2 is based on a combinatorial library of random heptapeptides fused to the N-terminus of a minor coat protein (pIII) of M13 phage. The peptide is followed by a short spacer (Gly-Gly-Gly) which directly precedes the wild-type pIII sequence. The library consists of ~1E09 electroporated sequences amplified once to yield approximately 100 copies of each sequence in 10 µl of the supplied phage.
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PhiX174 Virion DNA, New England Biolabs
Supplier: New England Biolabs (NEB)
ΦX174 Virion DNA is the single-stranded viral DNA isolated from bacteriophage ΦX174 am3 cs70
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pTYB21 Vector, New England Biolabs
Supplier: New England Biolabs (NEB)
pTYB21 is an E
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VSV-Pseudovirus_SARS-COV-2 Omicron CH.1.1 Luciferase
Supplier: ReVacc Scientific
This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the S protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations initially identified in variant of Omicron CH.1.1. The S has 18-aa cytoplasmic tail truncation for optimal infection.
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Unmethylated Lambda DNA, New England Biolabs
Supplier: New England Biolabs (NEB)
Duplex DNA is isolated from bacteriophage lambda (cI857ind 1 Sam 7)
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Lambda DNA, New England Biolabs
Supplier: New England Biolabs (NEB)
Duplex DNA is isolated from bacteriophage lambda (cI857ind 1 Sam 7)