Competent cells are those that have had their cell walls altered to render it easier to incorporate foreign DNA into their interiors. Cells are made competent by a process that uses calcium chloride and heat shock, and cells that are undergoing very rapid growth are made competent more easily than cells in other stages of growth. Factors such as transformation efficiency, genetic markers, and packaging formats will impact the time and effort required for your project, and so too its success. Choose from a number of cell types and formats to ensure optimal function.

SCSI Supercompetent Cells, Agilent Technologies

Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA

SCSI supercompetent and DH5α competent cells are classic E. coli strains engineered for competence.

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SHuffle T7 Express Competent E. coli, New England Biolabs

Supplier: New England Biolabs (NEB)

Chemically competent E. coli cells suitable for T7 protein expression with enhanced capacity to correctly fold proteins with multiple disulfide bonds in the cytoplasm.

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SHuffle Express Competent E.coli, New England Biolabs

Supplier: New England Biolabs (NEB)

Chemically competent E. coli cells suitable for non-T7 protein expression with enhanced capacity to correctly fold proteins with multiple disulfide bonds in the cytoplasm.

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BL21 Competent Cells, Agilent Technologies

Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA

BL21 competent cells are an all-purpose strain for high-level protein expression and easy induction.

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Lemo21(DE3) Competent E. coli, New England Biolabs

Supplier: New England Biolabs (NEB)

Chemically competent E. coli cells suitable for tunable T7 expression of challenging proteins.

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JM Competent Cells, Agilent Technologies

Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA

JM Competent Cells are recombination- and E. coli K12 restriction-proficient.

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SHuffle T7 Express lysY Competent E. coli, New England Biolabs

Supplier: New England Biolabs (NEB)

Chemically competent E. coli cells suitable for T7 protein expression with enhanced capacity to correctly fold proteins with multiple disulfide bonds in the cytoplasm and with enhanced reduction of basal expression.

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NEB®Stable Competent E.coli (High Efficiency), New England Biolabs

Supplier: New England Biolabs (NEB)

Chemically competent E. coli cells suitable for high efficiency transformation and isolation of plasmid clones containing repeat elements.

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Protein Expression Competent Cell Pack, Agilent Technologies

Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA

Protein Expression competent cell packs efficiently test multiple strains for expression.

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XL1 Blue and XL2 Blue Competent Cells, Agilent Technologies

Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA

XL1 and XL2-Blue Competent Cells are versatile for routine cloning and allow blue-white color screening for high efficiency cloning of methylated DNA.

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SURE Competent Cells, Agilent Technologies

Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA

SURE 2 competent cells are ideal for routine cloning of DNA with secondary structures.

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TG1 Electroporation-Competent Cells, Agilent Technologies

Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA

Phage display libraries from electroporation-competent cells with high transformation efficiency.

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SCS110 Competent Cells, Agilent Technologies

Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA

SCS110 competent cells for generating unmethylated DNA improve quality and yield of miniprep DNA.

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EletroTen-Blue Electroporation-Competent Cells, Agilent Technologies

Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA

Achieve the highest transformation efficiencies available when cloning large plasmids and ligated DNA.

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BJ5183 Electroporation-Competent Cells, Agilent Technologies

Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA

BJ5183 electroporation competent cells are a recombination proficient bacterial strain.

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XL-1 Red Competent Cells, Agilent Technologies

Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA

XL-1 Red Competent Cells are ideal for introducing random mutations into a cloned gene of interest.

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BL21 Competent Cells, Agilent Technologies

Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA

BL21 competent cells are an all-purpose strain for high-level protein expression and easy induction.

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SHuffle T7 Competent E. coli, New England Biolabs

Supplier: New England Biolabs (NEB)

Chemically competent E. coli K12 cells suitable for T7 protein expression with enhanced capacity to correctly fold proteins with multiple disulfide bonds in the cytoplasm.

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ABLE C Competent Cells, Agilent Technologies

Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA

ABLE Competent Cells for toxic clones reduce copy number four to ten fold compared to the XL1-Blue strain.

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Competent Cell Packs, Agilent Technologies

Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA

High efficiency competent cells in convenient packaging designed to satisfy higher throughput requirements.

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XL10-Gold Ultracompetent Cells

Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA

XL10-Gold ultracompetent cells transform large plasmids and ligated DNA with highest efficiency.

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NEB®5-alpha Competent E.coli (High Efficiency), New England Biolabs

Supplier: New England Biolabs (NEB)

NEB 5-alpha Competent E. coli (High Efficiency) is a derivative of the popular DH5. It is T1 phage resistant and endA deficient for high-quality plasmid preparations.

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ArcticExpress Competent Cells, Agilent Technologies

Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA

Tackle two common bacterial gene expression hurdles: protein insolubility and codon bias.

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TK Competent Cells, Agilent Technologies

Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA

Easily induce and express phosphorylated proteins, which can be used to screen expression libraries, to affinity purify or blot proteins that interact with the tyrosine-phosphorylated target.

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HI-Control™ Competent Cells, Biosearch Technologies

Supplier: Lucigen

Competent Cells include Control DNA and Recovery Medium, and are packaged as SOLOs (1 transformation per tube). Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA.

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Origami™ 2(DE3) Singles™ Competent Cells, MilliporeSigma

Supplier: MilliporeSigma

A common method for transformation of plasmids into E. coli is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen® prepared competent cells. Every Novagen® competent cell strain is verified for phenotype and purity, and is guaranteed for transformation efficiency.