You searched for: Sanger Sequencing Reagents

Sequencing reagents facilitate the processes when determining the precise order of nucleotides within DNA strands through sorting the fragments based on size. Enzymes are optimized to work together for high-efficiency removal of unincorporated primers and nucleotides. These reagents produce exceptional sequencing results from difficult templates, such as those with high GC content and secondary structures. Essential for basic and applied research procedures, sequencing reagents allow researchers to discover genotypes and phenotypes of specimen.

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illustra™ AutoScreen 96 Well Plates

Supplier: Cytiva

AutoScreen 96-well plate is used for purification of sequencing samples reactions prior to analysis on capillary array electrophoresis (CAE) sequencers such as MegaBACE 1000 or ABI Prism sequencing instruments.

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EnGen® sgRNA Synthesis Kits, S. pyogenes, New England Biolabs

Supplier: New England Biolabs (NEB)

In nature, S. pyogenes Cas9 is programmed with two separate RNAs, the crRNA and tracrRNA.

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Arginine-C™, Sequencing Grade, G-Biosciences

Supplier: G-Biosciences

SG-Arginine-C™ endopeptidase (Clostripain, from C. histolyticum) specifically hydrolyzes the carboxy peptide bond of Arginine. SG-Arginine-C™ has been modified chemically by a propriety process to render the enzyme resistant to autolysis and stabilize enzymatic activity. In addition, as a sulfhydryl enzyme, SG-Arginine-C™ is susceptible to inactivation by oxidation and as a result requires reducing agents for protection. The enzyme also requires calcium ion for maximal activity. A special reconstitution buffer is supplied, which contains reducing agents and activators to maintain enzyme activity.