Cloning Kits and Ligation
The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.
Pfu DNA Ligase, Agilent Technologies
Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA
Pfu DNA ligase is a recombinant enzyme derived from Pyrococcus furiosus hyperthermophilic marine archaebacterium that catalyzes linkage of adjacent 5'-phosphate and 3'-hydroxy ends of double-stranded DNA at 45 to 80 °C, with a temperature optimum near 70 °C for nick-sealing reactions.
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T4 DNA Ligase, Cytiva
Supplier: Cytiva
T4 DNA ligase catalyses the formation of a phosphodiester bond between the 5'-phosphoryl group and the 3'-hydroxyl group of two double-stranded DNA fragments. ATP is required for this reaction.
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BIOXP® 9600 Select Plasmid AMP
Supplier: TELESIS BIO
BioXp Select plasmid amplification kits contain reagents for isothermal amplification of plasmid DNA.
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StrataClone PCR Cloning Kits, Agilent Technologies
Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA
The StrataClone PCR Cloning Kit allows high-efficiency, 5-minute cloning of PCR products at room temperature, using the efficient DNA rejoining activity of DNA topoisomerase I and the DNA recombination activity of Cre recombinase.
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Random Mutagenesis Kits GeneMorph II, Agilent Technologies
Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA
The GeneMorph II random mutagenesis kits have been formulated to produce a more uniform mutational spectrum when performing error prone PCR to achieve random mutagenesis.
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Gibson Assembly® ULTRA Kits and Master Mixes (2X)
Supplier: TELESIS BIO
Generate seamless plasmid DNA or BAC clones without restriction digestion.
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BioXp® Select DNA Cloning Golden Gate Assembly BsmBI Kits
Supplier: TELESIS BIO
The Select DNA cloning Golden Gate Assembly BsmBI kits are compatible with the Type IIS restriction enzymes BsmBI. Please choose the appropriate kit based on the number of fragments being assembled.
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BioXp® Select DNA Cloning and Amplification Kits
Supplier: TELESIS BIO
The Select DNA cloning and amplification kits generate a minimum of 10 µg of amplified DNA from fragments of 0.3 to 1.8 kb using the BioXp® 3250.
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NEB® PCR Cloning Kit, New England Biolabs
Supplier: New England Biolabs (NEB)
This PCR Cloning Kit contains an optimized 2X Cloning Master Mix with a proprietary ligation enhancer and a linearized vector that uses a novel mechanism for background colony suppression to give a low background.
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BioXp® Select DNA Cloning Gibson Assembly Kits
Supplier: TELESIS BIO
The Select DNA cloning Gibson assembly kits can generate Gibson clones starting with fragments and vector supplied by the end user.
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Mutagenesis Kits QuikChange, Agilent Technologies
Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA
The original QuikChange Site-Directed Mutagenesis Kits speed up and simplify site-directed mutagenesis studies. The kits eliminate the need for sub-cloning into M13-based bacteriophage vectors and for ss-DNA rescue. This allows oligo-mediated introduction of site-specific mutations into virtually any double-stranded plasmid DNA. In addition, the XL version of the kit is specially designed for efficient mutagenesis of large (4 -14 kb) or otherwise difficult-to mutagenize plasmid templates. The XL kit features components specifically designed for more efficient DNA replication and bacterial transformation.
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Gigapack III Packaging Extracts, Agilent Technologies
Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA
For libraries constructed from highly methylated DNA, Gigapack III Packaging Extract simplifies the packaging procedure and increases the efficiency and representation of libraries constructed from highly methylated DNA. Each packaging extract is restriction minus to optimize packaging efficiency and library representation.
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Gibson Assembly® HIFI 1-Step Kits and Master Mixes (2X)
Supplier: TELESIS BIO
Generate seamless plasmid DNA or BAC clones without restriction digestion.
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NEB PCR Cloning Kit (Without Competent Cells), New England Biolabs
Supplier: New England Biolabs (NEB)
This PCR Cloning Kit contains an optimized Cloning Mix containing a proprietary ligation enhancer and a linearized vector that uses a novel mechanism for background colony suppression to give a low background.
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BioXp® Select DNA Cloning Golden Gate Assembly, BsaI Kits
Supplier: TELESIS BIO
The Select DNA cloning golden gate assembly, BsaI kits are compatible with the Type IIS restriction enzymes BsaI. Please choose the appropriate kit based on the number of fragments being assembled.
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BIOXP® 9600 Select Clone and AMP
Supplier: TELESIS BIO
The Select DNA cloning and amplification kits generate a minimum of 10 µg of amplified DNA from fragments of 0.3 to 1.8 kb using the BioXp 9600.
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Catalog #: CA21905-026
Supplier: Kimberly-Clark