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Protein Purification

Protein purification systems are intended to isolate one or multiple proteins from a complex mixture. Working with cells, tissues, or whole organisms, the machines purify with predefined start points and editable method templates. With a single click, the compact designed equipment offers real-time run control and visualization. Suitable for even cold-room operations, the products enable you to perform purification of affinity-tagged proteins, antibodies, untagged or native proteins, as well as sample clean-up.

EAH Sepharose™ 4B Affinity Coupling Media, Cytiva

EAH Sepharose™ 4B Affinity Coupling Media, Cytiva

Supplier: Cytiva

EAH Sepharose™ 4B allows for the stable carbodiimide-based coupling of carboxyl groups to Sepharose® 4B via an 11-atom hydrophilic spacer arm that permits extremely stable coupling.

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DEAE Sepharose™ Fast Flow Ion Exchange Chromatography Media, Cytiva

DEAE Sepharose™ Fast Flow Ion Exchange Chromatography Media, Cytiva

Supplier: Cytiva

DEAE Sepharose Fast Flow is a weak anion exchanger based on the well established Sepharose Fast Flow ion exchange platform, extensively used for preparative protein separations in both research and industrial applications.

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Sephadex™ G-10 Gel Filtration Media, Cytiva

Sephadex™ G-10 Gel Filtration Media, Cytiva

Supplier: Cytiva

Sephadex G-10 is a well established gel filtration medium for desalting and buffer exchange of peptides and small biomolecules >700 molecular weight.

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Streptavidin Sepharose™ High Performance Affinity Chromatography Media, Cytiva

Streptavidin Sepharose™ High Performance Affinity Chromatography Media, Cytiva

Supplier: Cytiva

Streptavidin Sepharose™ High Performance is an affinity chromatography medium designed for fast and reliable, high resolution purification of biotinylated biomolecules. Purified Streptavidin isolated from Streptomyces avidinii is immobilised on Sepharose™ High Performance.

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Sepharose™ CL-6B Gel Filtration Base Matrix

Sepharose™ CL-6B Gel Filtration Base Matrix

Supplier: Cytiva

Sepharose™ CL-6B is a cross-linked agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix.

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Superose™ 6 Gel Filtration Media, Prep Grade

Superose™ 6 Gel Filtration Media, Prep Grade

Supplier: Cytiva

Superose™ 6 PG for separations of proteins and other biomolecules according to size. Wide separation in two molecular weight ranges from 1000 up to 5 million.

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Q Sepharose™ High Performance Ion Exchange Chromatography Media, Cytiva

Q Sepharose™ High Performance Ion Exchange Chromatography Media, Cytiva

Supplier: Cytiva

Q Sepharose™ High Performance is composed of crosslinked agarose beads with a mean diameter of 34 µm, modified with quaternary (Q) strong anion exchange groups.

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MabSelect SuRe™ Affinity Chromatography Media, Cytiva

MabSelect SuRe™ Affinity Chromatography Media, Cytiva

Supplier: Cytiva

MabSelect SuRe™ is composed of a rigid, high-flow agarose matrix and alkali-tolerant rProtein A ligand. The ligand has been engineered to provide greater stability than conventional protein A-based media in the alkaline conditions used in CIP protocols. This enhanced alkali stability improves process economy and product quality.

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Blue Sepharose™ 6 Fast Flow Affinity Chromatography Media, Cytiva

Blue Sepharose™ 6 Fast Flow Affinity Chromatography Media, Cytiva

Supplier: Cytiva

Blue Sepharose™ 6 Fast Flow is cibacron blue 3G coupled to Sepharose™ 6 Fast Flow. It is well established as an adsorbent for albumin and interferon at both laboratory and process scale. The blue dye binds many proteins, such as albumin, interferon, lipoproteins and blood coagulation factors. It also binds several enzymes including kinases, dehydrogenases, and most enzymes requiring adenyl-containing cofactors e.g. NAD+.

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Sepharose™ 4B Gel Filtration Base Matrix

Sepharose™ 4B Gel Filtration Base Matrix

Supplier: Cytiva

Sepharose™ 4B is an agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling.

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nProtein A Sepharose™ 4 Fast Flow Affinity Chromatography Media, Cytiva

nProtein A Sepharose™ 4 Fast Flow Affinity Chromatography Media, Cytiva

Supplier: Cytiva

nProtein A Sepharose 4 Fast Flow is native protein A coupled to the well established Sepharose 4 Fast Flow base matrix.

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CM Sephadex™ C-25 Ion Exchange Chromatography Media, Cytiva

CM Sephadex™ C-25 Ion Exchange Chromatography Media, Cytiva

Supplier: Cytiva

Sephadex ion exchangers are produced by introducing functional groups onto the cross-linked dextran matrix.

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Epoxy-Activated Sepharose™ 6B Affinity Coupling Media, Cytiva

Supplier: Cytiva

Epoxy-activated Sepharose™ 6B is a pre-activated medium for immobilisation of various ligands including sugars through coupling of hydroxy, amino or thiol groups on the ligand to Sepharose™ 6B via a 12-atom hydrophilic spacer arm. Medium is formed by reacting Sepharose™ 6B with 1,4-bis (2,3- epoxy-propoxy-) butane. It can be used to couple sugars and other carbohydrates via stable ether linkages to hydroxyl groups.

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Superose™ 12 Gel Filtration Media, Prep Grade

Superose™ 12 Gel Filtration Media, Prep Grade

Supplier: Cytiva

Good resolution separations of proteins and other biomolecules according to size, Wide separation range – separations in two molecular weight ranges from 1,000 up to 5 million, Excellent reproducibility and durability

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CM Sepharose™ Fast Flow Ion Exchange Chromatography Media, Cytiva

CM Sepharose™ Fast Flow Ion Exchange Chromatography Media, Cytiva

Supplier: Cytiva

CM Sepharose Fast Flow is a weak cation exchanger based on the well established Sepharose Fast Flow ion exchange platform, extensively used for preparative protein separations in both research and industrial applications.

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Sephadex™ G-50 Gel Filtration Media, Cytiva

Sephadex™ G-50 Gel Filtration Media, Cytiva

Supplier: Cytiva

Sephadex G-50 Medium is well established gel filtration medium for desalting and buffer exchange of biomolecules >30 000 molecular weight.

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SOURCE™ 15S Ion Exchange Chromatography Media, Cytiva

SOURCE™ 15S Ion Exchange Chromatography Media, Cytiva

Supplier: Cytiva

SOURCE 15S is a synthetic high performance, preparative, chromatography medium, based on a 15 µm monosized, rigid polystyrene/divinyl benzene polymer matrix.

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IMAC Sepharose™ 6 Fast Flow Affinity Chromatography Media, Cytiva

IMAC Sepharose™ 6 Fast Flow Affinity Chromatography Media, Cytiva

Supplier: Cytiva

IMAC Sepharose 6 Fast Flow is composed of cross-linked 6% agarose beads modified with a novel chelating ligand immobilized to the base matrix.

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Chelating Sepharose™ Fast Flow Affinity Chromatography Media, Cytiva

Chelating Sepharose™ Fast Flow Affinity Chromatography Media, Cytiva

Supplier: Cytiva

Chelating Sepharose Fast Flow is composed of cross-linked 6% agarose beads modified with iminodiacetic immobilized to the base matrix by stable ether linkages and sufficiently long spacer arms.

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Sephadex™ LH-20 Gel Filtration Media, Cytiva

Sephadex™ LH-20 Gel Filtration Media, Cytiva

Supplier: Cytiva

Sephadex™ LH-20 is a liquid chromatography media for molecular sizing of natural product such as steroid, terpenoid, lipid and low molecular weight peptides. Sephadex™ LH-20 is pre-pared by hydroxypropylation of Sephadex™ G-25, a bead-formed dextran medium.

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MabSelect™ Affinity Chromatography Media

MabSelect™ Affinity Chromatography Media

Supplier: Cytiva

MabSelect is a protein A affinity resin designed for capturing mAbs from large sample volumes.

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NHS-Activated Sepharose™ 4 Fast Flow Affinity Coupling Media, Cytiva

NHS-Activated Sepharose™ 4 Fast Flow Affinity Coupling Media, Cytiva

Supplier: Cytiva

NHS-activated Sepharose™ 4 Fast Flow is composed of cross-linked 4% agarose beads, NHS activated for coupling amino-containing proteins and peptides in process-scale applications. NHS (N-hydroxysuccinimide) coupling forms a chemically stable amide bond with ligands containing primary amino groups. The resin provides a spacer arm to the coupled ligand and suitable for immobilising smaller protein and peptide ligands.

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Q Sepharose™ Fast Flow Ion Exchange Chromatography Media, Cytiva

Q Sepharose™ Fast Flow Ion Exchange Chromatography Media, Cytiva

Supplier: Cytiva

Q Sepharose Fast Flow is part of the Sepharose Fast Flow ion exchange platform, which has been the industrial standard for ion exchange chromatography during recent decades.

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SMART Digest™ ImmunoAffinity (IA) Streptavidin Kits

Supplier: Thermo Scientific

Obtain fast, simple, highly reproducible, and sensitive immunocapture and protein digestion in a single well using Thermo Scientific™ SMART Digest™ ImmunoAffinity (IA) Streptavidin Kits.

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Rabbit IgG Affinity Resin Kit, ImmunoReagents

Supplier: Immunoreagents

For the removal of cross-reactivity to rabbit immunoglobulins.

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Sepharose™ CL-2B Gel Filtration Base Matrix

Sepharose™ CL-2B Gel Filtration Base Matrix

Supplier: Cytiva

Sepharose CL-2B is a well-proven cross-linked agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling.

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Butyl-S Sepharose™ 6 Fast Flow Hydrophobic Interaction Chromatography Media, Cytiva

Butyl-S Sepharose™ 6 Fast Flow Hydrophobic Interaction Chromatography Media, Cytiva

Supplier: Cytiva

Butyl-S Sepharose™ 6 Fast Flow is composed of cross-linked 6% agarose beads modified with butyl groups via a thio-ether linkage. This resin is designed for the binding and elution of relatively strong hydrophobic molecules at comparatively low salt concentrations.

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Multiple Affinity Removal Starter Reagent Kit, Agilent Technologies

Multiple Affinity Removal Starter Reagent Kit, Agilent Technologies

Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA

Starter Reagent Kit for Multiple Affinity Removal LC Columns.

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Multiple Affinity Removal Spin Cartridge Reagent Kit, Agilent Technologies

Multiple Affinity Removal Spin Cartridge Reagent Kit, Agilent Technologies

Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA

The affinity removal spin cartridge reagent kit is designed to remove the most interfering high-abundant protein (albumin) from human samples (such as plasma, serum, urine or cerebrospinal fluid).

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Gel Filtration Calibration Kits, Cytiva

Gel Filtration Calibration Kits, Cytiva

Supplier: Cytiva

Gel Filtration Calibration Kits are suitable for calibration of high-resolution gel filtration media and columns such as Superdex, Superose, and Sephacryl.

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