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Protein Purification

Protein purification systems are intended to isolate one or multiple proteins from a complex mixture. Working with cells, tissues, or whole organisms, the machines purify with predefined start points and editable method templates. With a single click, the compact designed equipment offers real-time run control and visualization. Suitable for even cold-room operations, the products enable you to perform purification of affinity-tagged proteins, antibodies, untagged or native proteins, as well as sample clean-up.

CNBr-Activated Sepharose™ 4B Affinity Coupling Media, Cytiva

CNBr-Activated Sepharose™ 4B Affinity Coupling Media, Cytiva

Supplier: Cytiva

CNBr-activated Sepharose™ 4B is pre-activated media used for coupling antibodies or other large proteins containing -NH₂ groups to the Sepharose™ media, by the cyanogen bromide method, without an intermediate spacer arm.

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SP Sepharose™ Fast Flow Ion Exchange Chromatography Media, Cytiva

SP Sepharose™ Fast Flow Ion Exchange Chromatography Media, Cytiva

Supplier: Cytiva

SP Sepharose Fast Flow is a strong cation exchanger based on the well established Sepharose Fast Flow ion exchange platform, extensively used for preparative protein separations in both research and industrial applications.

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Capto™ adhere ImpRes Multimodal Chromatography Media, Cytiva

Capto™ adhere ImpRes Multimodal Chromatography Media, Cytiva

Supplier: Cytiva

Capto™ adhere ImpRes is a strong anion exchange multimodal BioProcess chromatography medium (resin), designed to meet the demands of modern large-scale manufacturers, for high-resolution polishing of monoclonal antibodies (MAbs) and other biomolecules. Capto™ adhere ImpRes is designed to allow effective MAb polishing in the second or third step of a purification scheme downstream of the protein A capture step. Polishing can be performed in either bind and elute (binding) or flowthrough (non binding) modes.

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MabSelect Xtra™ Affinity Chromatography Media, Cytiva

MabSelect Xtra™ Affinity Chromatography Media, Cytiva

Supplier: Cytiva

MabSelect Xtra has the same recombinant Protein A ligand as MabSelect but is based on a high-flow agarose base matrix with increased porosity and a slightly decreased particle size compared to MabSelect.

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Con A Sepharose™ 4B Affinity Chromatography Media, Cytiva

Supplier: Cytiva

Con A Sepharose is Concanavalin A coupled to Sepharose 4B by the cyanogen bromide method.

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Phenyl Sepharose™ 6 Fast Flow (High Sub) Hydrophobic Interaction Chromatography Media, Cytiva

Phenyl Sepharose™ 6 Fast Flow (High Sub) Hydrophobic Interaction Chromatography Media, Cytiva

Supplier: Cytiva

Phenyl Sepharose 6 Fast Flow is a well established, standard aromatic hydrophobic interaction chromatography (HIC) resin for capture and intermediate purification requiring low to resin hydrophobicity

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Lentil Lectin Sepharose™ 4B Affinity Chromatography Media, Cytiva

Supplier: Cytiva

Lentil lectin is a metalloprotein, containing Ca₂₊ and Mn₂₊, which binds reversibly to polysaccharides and glycoconjugates containing glucose or mannose type sugars.

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Sepharose™ CL-4B Octyl Hydrophobic Interaction Chromatography Media, Cytiva

Supplier: Cytiva

Octyl-Sepharose™ CL-4B is a well-proven medium for hydrophobic interaction chromatography (HIC). Octyl immobilised on 4% cross-linked agarose.

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Affinity purification media, GammaBind™ Plus Sepharose™

Supplier: Cytiva

GammaBind Plus Sepharose is GammaBind G, Type 3, covalently immobilized to Sepharose CL-6B by malimide linkage.

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Benzamidine Sepharose™ 4 Fast Flow (High Sub) Affinity Chromatography Media, Cytiva

Benzamidine Sepharose™ 4 Fast Flow (High Sub) Affinity Chromatography Media, Cytiva

Supplier: Cytiva

Benzamidine Sepharose 4 Fast Flow (high sub) is an excellent tool for removal and/or purification of trypsin and trypsin-like serine proteases, as well as, zymogens including urokinase and prekallikrein.

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ANX Sepharose™ 4 Fast Flow (High Sub) Ion Exchange Chromatography Media, Cytiva

ANX Sepharose™ 4 Fast Flow (High Sub) Ion Exchange Chromatography Media, Cytiva

Supplier: Cytiva

ANX Sepharose™ 4 Fast Flow (high sub) is a weak anion exchanger, with a different selectivity compared with traditional ion exchangers.

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IgG Sepharose™ 6 Fast Flow Affinity Chromatography Media, Cytiva

IgG Sepharose™ 6 Fast Flow Affinity Chromatography Media, Cytiva

Supplier: Cytiva

lgG Sepharose™ 6 Fast Flow, from Cytiva, is based on the rigid Sepharose 6 Fast Flow matrix, with human IgG covalently coupled to it.

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SP Sephadex™ C-25 Ion Exchange Chromatography Media, Cytiva

SP Sephadex™ C-25 Ion Exchange Chromatography Media, Cytiva

Supplier: Cytiva

SP Sephadex™ C-25 is a strong cation exchanger.

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QAE Sephadex™ Ion Exchange Chromatography Media, Cytiva

QAE Sephadex™ Ion Exchange Chromatography Media, Cytiva

Supplier: Cytiva

Sephadex ion exchangers are produced by introducing functional groups onto the cross-linked dextran matrix.

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DEAE Sephadex™ A-25 Ion Exchange Chromatography Media

DEAE Sephadex™ A-25 Ion Exchange Chromatography Media

Supplier: Cytiva

DEAE Sephadex™ A-25 and DEAE Sephadex™ A-50 are weak anion exchangers. Sephadex™ ion exchangers are produced by introducing functional groups onto the cross-linked dextran matrix. These groups are attached to glucose units in the matrix by stable ether linkages.

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Sephadex™ G-15 Gel Filtration Media, Cytiva

Sephadex™ G-15 Gel Filtration Media, Cytiva

Supplier: Cytiva

Sephadex G-15 is a well established gel filtration medium for desalting and buffer exchange of peptides and small biomolecules >1500 molecular weight.

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Streptavidin Sepharose™ High Performance Affinity Chromatography Media, Cytiva

Streptavidin Sepharose™ High Performance Affinity Chromatography Media, Cytiva

Supplier: Cytiva

Streptavidin Sepharose™ High Performance is an affinity chromatography medium designed for fast and reliable, high resolution purification of biotinylated biomolecules. Purified Streptavidin isolated from Streptomyces avidinii is immobilised on Sepharose™ High Performance.

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SMART Digest™ ImmunoAffinity (IA) Protein A Kits

Supplier: Thermo Scientific

Obtain fast, simple, highly reproducible, and sensitive immunocapture and protein digestion in a single well using Thermo Scientific™ SMART Digest™ ImmunoAffinity (IA) Protein A Kits.

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Sephadex™ G-75 Gel Filtration Media, Cytiva

Sephadex™ G-75 Gel Filtration Media, Cytiva

Supplier: Cytiva

Sephadex G-75 is well established gel filtration medium for desalting and buffer exchange of large biomolecules >80 000 molecular weight.

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Superdex™ Prep Grade Gel Filtration Media, Cytiva

Superdex™ Prep Grade Gel Filtration Media, Cytiva

Supplier: Cytiva

Superdex prep grade is a preparative gel filtration medium with a composite matrix of dextran and agarose

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Sepharose™ 6B Gel Filtration Base Matrix

Sepharose™ 6B Gel Filtration Base Matrix

Supplier: Cytiva

Sepharose™ 6B is an agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling.

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MabSelect™ VL Protein L Resin, Cytiva

MabSelect™ VL Protein L Resin, Cytiva

Supplier: Cytiva

MabSelect™ VL resin with a redesigned protein L ligand for high binding capacity and improved alkaline stability. For affinity capture of antibody Fabs, bispecific, and other variants containing a kappa light chain.

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SOURCE™ 30S Ion Exchange Chromatography Media, Cytiva

SOURCE™ 30S Ion Exchange Chromatography Media, Cytiva

Supplier: Cytiva

SOURCE 30S is a synthetic high performance, preparative, chromatography medium, based on a 30 µm monosized, rigid polystyrene/divinyl benzene polymer matrix It is modified with sulphonate (S) strong cation exchange groups.

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CNBr-Activated Sepharose™ 4 Fast Flow Affinity Coupling Media, Cytiva

CNBr-Activated Sepharose™ 4 Fast Flow Affinity Coupling Media, Cytiva

Supplier: Cytiva

CNBr-activated Sepharose™ 4 Fast Flow is based on the Sepharose™ Fast Flow platform. The resin is composed of cross-linked 4% agarose beads that has been pre-activated with cyanogen bromide.

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SOURCE™ 15RPC Reversed Phase Chromatography Media, Cytiva

SOURCE™ 15RPC Reversed Phase Chromatography Media, Cytiva

Supplier: Cytiva

SOURCE™ 15RPC is designed for reversed phase chromatography (RPC) with full pH range stability and suitable for polishing steps in industrial processes requiring high flow velocities and low back-pressure.

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nProtein A Sepharose™ 4 Fast Flow Affinity Chromatography Media, Cytiva

nProtein A Sepharose™ 4 Fast Flow Affinity Chromatography Media, Cytiva

Supplier: Cytiva

nProtein A Sepharose 4 Fast Flow is native protein A coupled to the well established Sepharose 4 Fast Flow base matrix.

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CM Sephadex™ C-25 Ion Exchange Chromatography Media, Cytiva

CM Sephadex™ C-25 Ion Exchange Chromatography Media, Cytiva

Supplier: Cytiva

Sephadex ion exchangers are produced by introducing functional groups onto the cross-linked dextran matrix.

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SOURCE™ 30Q Ion Exchange Chromatography Media, Cytiva

SOURCE™ 30Q Ion Exchange Chromatography Media, Cytiva

Supplier: Cytiva

SOURCE™ 30Q is a synthetic high performance, preparative, chromatography medium, based on a 30 µm monosized, rigid polystyrene/divinyl benzene polymer matrix.

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DEAE Sephadex™ A-50 Ion Exchange Chromatography Media, Cytiva

DEAE Sephadex™ A-50 Ion Exchange Chromatography Media, Cytiva

Supplier: Cytiva

DEAE Sephadex™ A-25 and DEAE Sephadex™ A-50 are weak anion exchangers. Sephadex™ ion exchangers are produced by introducing functional groups onto the cross-linked dextran matrix. These groups are attached to glucose units in the matrix by stable ether linkages.

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Sepharose™ CL-6B Gel Filtration Base Matrix

Sepharose™ CL-6B Gel Filtration Base Matrix

Supplier: Cytiva

Sepharose™ CL-6B is a cross-linked agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix.

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