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  | Avantor

Restriction Enzymes

Nucleic acid reagents form the foundation of genetic analysis and molecular biology, playing a pivotal role from cloning to gene expression studies. Delve into our collection featuring high-fidelity enzymes for end-point PCR, advanced reagents for isothermal amplification, and comprehensive kits for nucleic acid purification. For precision in quantitative assays, explore our selection of qPCR and RT-qPCR kits, primed for unparalleled accuracy. Our portfolio also includes next-generation sequencing reagents, offering cutting-edge solutions for genomic sequencing and personalized medicine advancements.

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Live Restriction Enzymes

Supplier: Avantor

These restriction enzymes are used to recognize specific sequences in double strand DNA. BamH I recognizes 5-G/GATCC-3, Ecor I recognizes 5-G/AATTC-3, and Hind III recognizes 5-A/AGCTT-3. The activity level of all our restriction enzymes is 10 units per 1 µl.
All are shipped next day on ice. As they are perishable, a ship date must be specified.

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Dpn I, Restriction Enzymes, Agilent Technologies

Dpn I, Restriction Enzymes, Agilent Technologies

Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA

Dpn I is an ideal enzyme for cloning and southern blot analysis.

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NruI-HF, Restriction Enzymes, New England Biolabs

NruI-HF, Restriction Enzymes, New England Biolabs

Supplier: New England Biolabs (NEB)

NruI-HF is produced in E. coli from a strain that carries the cloned and modified NruI gene from Nocardia rubra (ATCC 15906).

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Hpy166II

Hpy166II

Supplier: New England Biolabs (NEB)

An E. coli strain that carries the cloned Hpy166II gene from Helicobacter pylori J166 (M.J. Blaser).

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MboII

MboII

Supplier: New England Biolabs (NEB)

An E.coli strain that carries the cloned MboII gene from Moraxella bovis (ATCC 10900).

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NsiI

NsiI

Supplier: New England Biolabs (NEB)

An E.coli strain that carries the NsiI gene from Neisseria sicca (ATCC 29256).

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AlwNI

AlwNI

Supplier: New England Biolabs (NEB)

An E.coli strain that carries the cloned AlwNI gene from Acinetobacter Iwoffii N (R.Morgan).

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MluI-HF, Restriction Enzymes, New England Biolabs

MluI-HF, Restriction Enzymes, New England Biolabs

Supplier: New England Biolabs (NEB)

MluI-HF is produced in E. coli from a strain that carries the cloned and modified MluI gene from Micrococcus luteus (IFO 12992)

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AclI

AclI

Supplier: New England Biolabs (NEB)

An E.coli strain that carries the AclI gene from Acinetobacter calcoaceticus M4 (S.K. Degtyarev).

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BstEII-HF®, New England Biolabs, Restriction Enzymes, New England Biolabs

BstEII-HF®, New England Biolabs, Restriction Enzymes, New England Biolabs

Supplier: New England Biolabs (NEB)

BstEII-HF® is produced in E. coli from a strain that carries the cloned and modified BstEII-HF gene from Bacillus stearothermophilus ET (N. Welker).

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BssSI-v2

BssSI-v2

Supplier: New England Biolabs (NEB)

An E.coli strain that carries the cloned and modified BssSI gene from Bacillus stearothermophilus S719 (Z. Chen).

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MwoI

MwoI

Supplier: New England Biolabs (NEB)

An E.coli strain that carries the MwoI gene from Methanobacterium wolfeii (DSM 2970).

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BamHI-HF® Restriction Enzymes, New England Biolabs

BamHI-HF® Restriction Enzymes, New England Biolabs

Supplier: New England Biolabs (NEB)

An E.coli strain that carries the cloned and modified BamHI gene from Bacillus amyloliquefaciens H (ATCC 49763). High Fidelity (HF®) Restriction Enzymes have 100% activity in CutSmart® Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver™ qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.

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MmeI

MmeI

Supplier: New England Biolabs (NEB)

An E.coli strain that carries the MmeI gene from Methylophilus methylotrophus.

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HpyCH4V

HpyCH4V

Supplier: New England Biolabs (NEB)

An E. coli strain that carries the cloned HpyCH4V gene from Helicobacter pylori CH4 (S.A. Thompson).

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TspRI

TspRI

Supplier: New England Biolabs (NEB)

An E.coli strain that carries the cloned TspRI gene from Thermus species R (R.A.D. Williams).

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