You can learn more about a particular area of chromatography. We have a range of educational information available for you.
This knowledge note demonstrates how to achieve a high-yield preparative scale separation and confirm the purity of the resulting isolated fractions.
This article uses isocratic and gradient examples to demonstrate how significant reductions in mobile phase consumption and waste can be achieved by migrating existing methods onto smaller column formats which maintaining chromatographic results.
This knowledge note highlights how reducing the LC column internal diameter can help significantly reduce the environmental impact of liquid chromatography moving towards more sustainable laboratory practices.
This knowledge note reviews and compares the recent changes to allowable adjustments made by the USP. This includes the significant revisions that have been made to the guidance for gradient methods.
Extra column volume and dispersion can seriously affect column performance. Here we explain why, how to experimentally measure and approaches to help reduce their impact.
Have you ever been confused as to the best solution to a certain separation problem? We explain the effect of these parameters on performance.
If you are looking for a clear introduction to the practicalities of HILIC, this is a comprehensive guide for those unfamiliar with this mode of HPLC.
Correct care and use is essential to maximise the lifetime of any (U)HPLC column. This Knowledge note summarises simple routine practices that should be followed to maximise performance.
This article compares the characteristics of Methanol & Acetonitrile, such as UV cut-off, pressure and elution order before discussing how organic solvent selection can be a powerful method development tool.
Some degree of band broadening is unavoidable, however this Knowledge note looks at the underlying theory in order to help minimise it’s impact on methods.
Analyte pKa, logP and logD measurements are useful parameters that can help determine the most suitable mode of chromatography, aid in diluent and mobile phase selection and help diagnose various issues such as poor peak shape.
This Knowledge Note outlines a simple and systematic protocol for screening new samples using reversed-phase conditions, that can help rationalise and streamline the development of new LC methods.
This article demonstrates how throughput for existing isocratic and gradient analytical LC methods can be increased using existing HPLC instruments and, where applicable, by upgrading to UHPLC.
This short article examines different column diameters and the benefits they may provide for UV and MS analyses. Commonly observed reductions in method performance with smaller ID columns are also illustrated, and the proposed solutions to recapture metho
This Knowledge Note discusses the key parameters which require attention and shows how the Avantor® ACE® LC Translator can assist in simplifying the process to help achieve reliable method transfer.
This Knowledge Note summarises the allowable changes permitted for LC methods published in the USP and Ph. Eur. and additionally, demonstrates how these can be utilised to speed up methods and improve laboratory throughput.
This Knowledge Note explains how retention of acidic, basic and neutral analytes is affected by mobile phase pH, as well as the requirements for carrying out separations at high and low pH.
This Knowledge Note discusses the impact that column temperature can have on LC separations, why it is important to consider during method development and limitations to avoid column and sample stability issues.