"qPCR Mastermixes"

Supplier: Biotium

A qPCR master mix containing Evagreen® qPCR dye and Cheetah™ Taq hotstart DNA polymerase, suitable for qPCR using a fast cycling protocol.

Supplier: VWR Chemicals

VWR® TEMPase Hot Start DNA Polymerases are highly stable polymerases, featuring higher specificity, superior sensitivity and greater yields compared to standard DNA polymerases. These features make them well suited for the detection of low abundance targets. Other uses include screening, amplification of GC-rich sequences, multiplex PCR, direct PCR and qPCR.

Supplier: PRIMERDESIGN

The oasig freeze drying process stabilises all of the active components allowing these reagents to be shipped and stored at room temperature. This hugely simplifies the logistics of purchasing, shipping and using the technology. Whether you are in a sophisticated laboratory in Texas or a mobile field hospital in Timbuktu we can supply complete qPCR kit and reagent packages to your door quickly and cheaply via standard shipping methods without the need for dry ice or a cold chain of any sort.
Freeze dried qPCR reagents stored and shipped at ambient temperatures:

Supplier: Quantabio

qScript™ XLT One-Step RT-qPCR ToughMix® is een gebruiksklare, zeer gevoelige formule voor kwantitatieve omgekeerde transcriptie-PCR (RT-qPCR) van RNA-templates.

Supplier: AGILENT

The Brilliant HRM Ultra-Fast Loci Master Mix for High Resolution Melt (HRM) analysis.

Supplier: Thermo Fisher Scientific

ABsolute Blue™ Master Mix bevat een inerte blauwe kleurstof om het contrast tussen reagens en kunststof aanzienlijk te vergroten, waardoor de verificatie van Master Mix-doseringen snel, gemakkelijk en zonder problemen kan worden uitgevoerd. Toepassingen zijn onder meer genexpressie, siRNA-validatie, bepaling van SNP-type en genotype.

Supplier: Quantabio

Deze SuperMix is een 2x geconcentreerde, gebruiksklare reactiecocktail voor realtime qPCR die alle componenten bevat behalve primers, probes en DNA templates. Het systeem overstijgt de multiplexbeperkingen van conventionele PCR-mengsels, waardoor zuivere amplificatie van maximale vijf targetsequenties in één buisje mogelijk is. Onderdrukking van ampliconen met een beperkt aantal kopieën door referentietargets met veel kopieën in de amplificatie is een veel voorkomend probleem bij multiplex-PCR. Hierdoor kan de schijnbare representatie en kwantificering van doelsequenties met een beperkt aantal kopieën worden vertekend of gemaskeerd. PerfeCTa® MultiPlex qPCR SuperMix geeft een dynamisch bereik en gevoeligheid aan multiplex-qPCR die vergelijkbaar zijn met die voor probe-analyses van enkelvoudig qPCR, zonder dat er beperkende of variabele primerconcentraties nodig zijn.

Supplier: Quantabio

Synthesis of cDNA and PCR amplification are carried out in the same tube without opening between procedures. These kits are optimised to deliver maximum RT-PCR efficiency and specificity, enabling unbiased co-amplification of low copy transcripts in the presence of higher copy reference genes. As a result the qScript™ One-Step qRT-PCR Kits offer improved sensitivity over other commercially available qPCR reagents. The qScript™ One-Step Master Mix maximises the activities of both reverse transcriptase and PCR enzymes, while minimising primer-dimer formation. High specificity is assured with AccuStart™ Taq DNA Polymerase, which is bound by monoclonal antibodies for superior hot-start. Upon heating to 95 °C, the antibodies denature irreversibly releasing fully active and unmodified Taq DNA polymerase. qScript™ One-Step qRT-PCR Kits are available for all Real-Time PCR instrument platforms, including those requiring normalisation with ROX reference dye.

Supplier: Enzo Life Sciences

Rapid removal of contaminating DNA in PCR mastermixes, without reduction in PCR sensitivity.

Supplier: Quantabio

Ultra-Plex™ 1-Step ToughMix® is a ready-to-use, 4X-concentrated master mix for reverse transcription quantitative PCR (RT-qPCR) of RNA templates using hybridisation probe detection chemistries such as TaqMan® 5’-hydrolysis probes on Real-Time PCR systems that utilise ROX passive reference with 490 nM excitation. First-strand cDNA synthesis and PCR amplification are carried out in the same tube without opening between procedures. It is ideal for highly sensitive quantification of RNA viruses or low abundance RNA targets in uni- or multiplexed RT-qPCR applications as well as high throughput gene-expression studies. The system has been optimised to deliver maximum RT-qPCR efficiency, sensitivity, and specificity in reduced reaction volumes and fast cycle times. Ultra-Plex™ 1-Step ToughMix® contains all required components for RT-qPCR except RNA template and probe. It is compatible with all dual-labeled probe chemistries.

Supplier: Thermo Fisher Scientific

Het Verso™ qRT-PCR systeem combineert het Verso™ RT-enzym met ABsolute™ Blue qPCR Master Mix voor maximaal gemak en optimale veelzijdigheid. Verkrijgbaar in formaat met 1 en 2 stappen, alsmede met meerdere verpakkingsgrootten, om te kunnen voldoen aan alle toepassings- en doorvoereisen.

Supplier: Thermo Fisher Scientific

The Maxima™ SYBR® Green qPCR Master Mix (2X) is a ready to use solution optimised for quantitative real time PCR and two-step real time RT-PCR on most real time PCR machines. The master mix includes Maxima™ hot start Taq DNA polymerase, SYBR® Green dye I and dNTPs in an optimised PCR buffer. ROX solution is provided in a separate vial for use with machines that require ROX as a passive reference dye. Maxima™ hot start Taq DNA polymerase in combination with the optimised buffer ensures PCR specificity and sensitivity. The SYBR® Green I intercalating dye allows for DNA detection and analysis without using sequence specific probes. dUTP is included in the mix for optional carryover contamination control using uracil-DNA glycosylase (UDG). The use of Maxima™ SYBR® Green qPCR Master Mix (2X) in real time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates.

Supplier: Stemcell Technologies

For probe-based assays and arrays.

Supplier: Quantabio

sparQ Universal Library Quant Kit provides rapid and accurate quantification of libraries prepared for sequencing on Illumina® NGS platforms.

Supplier: QIAGEN

RT SYBR GREEN QPCR MASTERMIX 1 * 1 ST

Supplier: QIAGEN

RT SYBR GREEN QPCR MASTERMIX (2) 1 * 1 Kit