"protein purification system"

Supplier: Thermo Fisher Scientific

A solution of glutathione-S-transferase (≥100 units/mg).

Supplier: Cytiva

Tube holder, Polyoxymethylene

Supplier: Abcam

Abcam's ChIP Kit Magnetic - One Step (ab156907) is a complete set of optimized buffers and reagents to carry out a successful chromatin immunoprecipitation for the selective enrichment of a chromatin fraction containing specific DNA sequences from isolated chromatin, in order to investigate the interaction between proteins and DNA in a cell.

Supplier: Thermo Fisher Scientific

Pierce™ Protein IgG Plus Orientation Kits uses a crosslinker to covalently attach antibody that is bound to Protein A (or Protein G) agarose resin, thereby creating a reusable affinity column for purification of antigens.

Supplier: Cytiva

Cobalt-based chromatography medium designed for purification of histidine-tagged recombinant proteins by immobilised metal affinity chromatography (IMAC).

Supplier: Thermo Fisher Scientific

Pierce glutathione coated plates are coated with intact glutathione (GSH) and pre-blocked for use in capture and detection assays involving glutathione-S-transferase (GST) fusion proteins. These coated plates can be used in the analysis of cell lysates to determine the presence and concentration of the GST-fusion protein produced in an expression system. Plates can also be used to capture and coat fusion proteins via their GST tag for subsequent binding assays involving the recombinant protein. As the plates are pre-blocked, initial purification of the cell lysates is not necessary.

Supplier: Thermo Fisher Scientific

Pierce™ Protein A/G agarose is a affinity resin for antibody purification.

Supplier: OMEGA BIO-TEK

RNA-Solv® Reagent is a one reagent system for the isolation of total RNA from cells and tissues. The reagent, a single-phase solution consisting of phenol and guanidine isothiocyanate, is a modification of the single-step RNA isolation method developed by Chomczynski and Sacchi. The sample is homogenised and lysed in RNA-Solv® Reagent, which maintains the integrity of the RNA while disrupting and denaturing endogenous RNases and other cellular components. Extraction of the lysate with chloroform further denatures proteins and separates the mixture into an organic and an aqueous phase. RNA remains exclusively in the aqueous phase, and is subsequently recovered by isopropanol.

Supplier: Abcam

APC conjugation / labeling in < 4 hrs with 30 secs hands-on time using APC Conjugation Kit ab201807. 100% antibody recovery. - Cited in over 50 publications - APC labeled antibody is ready for use in applications like WB, ELISA, and IHC, with no need for further purification - Compatible with most standard antibody formulations - Scalable;use the same conjugation method from 10µg-100mg of antibody - Use Lightning-Link® conjugation with over 45 labels, including Alexa Fluor®, DyLight®, tandem dyes, enzymes, oligonucleotides, and gold nanoparticles

Supplier: Thermo Fisher Scientific

The Pierce™ Melon™ Gel IgG Spin Purification Kit is a simpler and faster alternative to traditional Protein A and Protein G methods for purification of human, mouse and rabbit IgG from serum and other sources.

Supplier: G-Biosciences

Streptavidin Resin is designed for the single step small and large scale affinity purification of proteins and antibodies with a biotin tag. Biotin, a 244 Da vitamin (Vitamin H) molecule, exhibits an extraordinary binding affinity for avidin (Ka=10¹⁵ M⁻¹) and streptavidin (Ka=10¹⁵ M⁻¹). Biotin and (strept)avidin interaction is rapid and once the bond is established it can survive up to 3M guanidinehydrochloride and extremes of pH. Biotin-avidin bonds can only be reversed by denaturing the avidin protein molecule with 8M guanidine-hydrochloride at pH 1,5 or by autoclaving. The biotinylated molecules are efficiently probed with avidin or streptavidin conjugated to reporter molecules, such as peroxidases or phosphatases.

Supplier: Thermo Fisher Scientific

The Melon™ Gel Spin Plate Kit for IgG Screening offers a high throughput format for quick purification and screening of antibodies.

Supplier: Thermo Fisher Scientific

Protein A/G magnetic beads, ChIP grade, 5 ml

Supplier: Thermo Fisher Scientific

Pierce™ Protein G agarose is a premium quality affinity resin for antibody purification.

Supplier: Cytiva

Clips

Supplier: Cytiva

Biacore maintenance kits are convenient kits for proper instrument maintenance.