"Spectrophotometer"

Supplier: Cole-Parmer

SPECTROPHOTOMETER GENOVA NANO UV/VIS 1 * 1 ST

Supplier: Thermo Fisher Scientific

Pierce BCA™ (bicinchoninic acid) reagents provide accurate determination of protein concentration with most sample types encountered in protein research. The Pierce BCA™ Protein Assay can be used to assess yields in whole cell lysates and affinity-column fractions, as well as to monitor protein contamination in industrial applications. Compared to most dye-binding methods, the BCA™ Assay is affected much less by protein compositional differences, providing greater protein-to-protein uniformity.

Supplier: EDVOTEK

When DNA is subcloned in the pUC polylinker region, β-galactosidase production is interrupted, resulting in the inability of cells to hydrolyse X-Gal. This results in the production of white colonies amongst a background of blue colonies. This experiment provides a DNA fragment together with a linear plasmid and T4 DNA ligase. Following the ligation to synthesis the recombinant plasmid, competent E. coli cells are transformed and the number of recombinant antibiotic-resistant white and blue colonies are counted. β-galactosidase activity is assayed from blue and white bacterial cells.

Supplier: Cole-Parmer

SPECTROPHOTOMETER GENOVA PLUS UV/VIS 1 * 1 ST

Supplier: Cole-Parmer

[EN]DUST COVER FOR 7315 SPECTROPHOTOMETE 1 * 1 ST

Supplier: Cole-Parmer

SPECTROPHOTOMETER UV/VIS MODEL 6305 1 * 1 ST

Supplier: OPTIGLASS

[EN]STARNA SPECTROPHOTOMETER FLOW CELLS 1 * 1 ST

Supplier: BIOCHROM

[EN]SPECTROPHOTOMETER BIOWAVE 3+W/BT+PRT 1 * 1 ST

Supplier: MARKET SOURCE PART PROCESS

[EN]1 µL UV-VIS SPECTROPHOTOMETER 1 * 1 ST

Supplier: Thermo Fisher Scientific

The Thermo Scientific Pierce™ Chromogenic endotoxin Quant kit is a highly sensitive endpoint assay that accurately measures and detects endotoxin (lipopolysaccharide) in a protein, peptide, nucleic acid, or antibody sample using the amebocyte lysate assay. The kit enables detection within two linear sensitivity ranges of 0,01 to 0,1 EU/ml and 0,1 to 1,0 EU/ml.

Supplier: BIOCHROM

[EN]SPECTROPHOTOMETER BIOWAVE 3W/BT+PRTB 1 * 1 ST

Supplier: G-Biosciences

Biodiversity lab activity is designed for determination of protein contents and biomass in diverse biological samples to study how biomass is related to biodiversity in nature. Students collect and catalogue plant leaf samples from a diverse group of locally available plants. This lab activity involves determination of natural weight of each plant sample, grinding a predetermined amount of each sample, and the subsequent extraction of proteins from the samples. Students then learn to determine protein contents of each plant sample and attempt to relate the protein content with biomass. Students in this lab activity are challenged to think, analyse, and seek answers as to why protein biomasses vary for a given natural weight for different plants. Finally, they will relate that finding to the biodiversity of nature.

Supplier: ANVAJO

The fluidlab R-300 combines an automated cell counter with a full-fledged spectrometer, thus enabling a variety of analyses such as automated cell counting and staining-free viability measurements as well as scatter light, absorbance and time-series measurements. With its intuitive handling and reliable results, the portable device is the all-rounder for your everyday laboratory work.

Supplier: Thermo Fisher Scientific

The Mammalian β-Galactosidase Assay Reagent Kit is a simple and versatile assay to measure β-galactosidase activity in cultured mammalian cells. Cells grown on a plate may be lysed and tested for β-galactosidase activity by adding a single all-in-one lysis-and-assay solution. Alternatively, M-PER™ Mammalian Protein Extraction Reagent may be used to prepare lysate from washed adherent or pelleted cells, and then a portion of the lysate used to assay for β-galactosidase activity. With M-PER Reagent, there is no need to scrape cells or to perform freeze-thaw cycles. The remaining lysates prepared with M-PER™ Reagent may be used for applications such as reporter assays (luciferase, β-galactosidase and chloramphenical acetyltransferase), protein kinase assays (PKA, PKC, and tyrosine kinase), immunoassays (Western blot, ELISA, RIA) and protein purification.

Supplier: Lovibond

The SensoDirect 150 is designed for multi purpose operation and measures pH/Redox, dissolved oxygen and conductivity/TDS.

Supplier: G-Biosciences

Determination of protein concentration is an essential technique in all aspects of protein studies and proteomics. The Assays for Protein Quantification kit includes three of the most widely used protein assays and allows for a direct comparison of the three assays that teaches students the benefits and limitations of each assay. Each assay is available individually to allow teaching of a specific assay, without the option of comparing and contrasting with other assays. The three assays covered are the Biuret Protein Assay, Lowry Protein Assay and the Coomassie Blue Dye Protein Assay. The Assays for Protein Quantification kit provides all the reagents required to perform both protein assays, including protein standards for accurate quantification, in a single lab activity. An often underestimated factor in quantifying protein is the presence of non-protein interfering agents, such as salts and detergents. This kit teaches students about common laboratory agents that affect the protein assays, the reasoning behind their interferences and how to overcome the interference. Students also learn how to select a protein assay for different applications.