"Spectrophotometer"

Supplier: G-Biosciences

Ion exchange chromatography is used to separate charged molecules from complex biological samples. The charged molecules bind to a solid support carrying an opposite charge to the molecule. Proteins contain regions of charged groups on the surface which interact with the ion exchange groups immobilised on the solid support (resin column). Immobilized proteins are eluted by changing either pH or the salt gradient or a combination of both. This lab activity involves preparation of a crude protein extract and running ion exchange chromatography for isolation of proteins.

Supplier: Cole-Parmer

SPECTROPHOTOMETER 7305 UV/VISIBLE 1 * 1 ST

Supplier: OPTIGLASS

[EN]SPECTROPHOTOMETER CELL W. STOPPER 1 * 1 ST

Supplier: BIOCHROM

[EN]SPECTROPHOTOMETER BIOWAVE 3+W/PRINTE 1 * 1 ST

Supplier: Lovibond Tintometer

SPECTROPHOTOMETER TRA520 AND ACC. KIT 1 * 1 ST

Supplier: Cole-Parmer

SCANNING SPECTROPHOTOMETER 7200 VISIBLE 1 * 1 ST

Supplier: Cole-Parmer

SPECTROPHOTOMETER 7315 UV/VISIBLE 1 * 1 ST

Supplier: DIDALAB

[EN]FIBRE OPTIC SPECTROPHOTOMETER 1 * 1 ST

Supplier: Thermo Fisher Scientific

[EN]WIFI DONGLE FOR SPECTROPHOTOMETERS 1 * 1 ST

Supplier: OPTIGLASS

[EN]CELL F. SPECTROPHOTOMETER CERTIFIED 1 * 1 ST

Supplier: Hach

[EN]LIGHT SHIELD F SPECTROPHOTOMETER DR3 1 * 1 ST

Supplier: Molecular Devices

[EN]PLUS384 UV/VIS CUVETTE/MICROPLATE RE 1 * 1 ST

Supplier: Thermo Fisher Scientific

[EN]SPECTRONIC 200E VIS SPECTROPHOTOMETE 1 * 1 ST

Supplier: Cole-Parmer

SPECTROPHOTOMETER UV/VIS MODEL 6305 1 * 1 ST

Supplier: G-Biosciences

NUCLEIC dotMETRIC™ Kit provides a quick and accurate method for detecting and measuring nucleic acid concentration, including DNA, RNA and oligonucleotides. The system uses a combination of proprietary reagents and test strips and is an alternative to spectrophotometry as no equipment or cuvettes are necessary. NUCLEIC dotMETRIC™ uses as little as one microlitre of sample and provides permanent results within 2 to 3 minutes.

Supplier: EDVOTEK

In dit experiment voeren de leerlingen een zuivering van het restrictie-enzym Eco RI uit. Bij deze procedure wordt gebruikgemaakt van een stap met ionenuitwisselingschromatografie voor het zuiveren van Eco RI. De kolomfracties worden getoetst op het enzym met behulp van lambda-DNA en de verteringsproducten worden geïdentificeerd door middel van gelelektroforese. Fracties die Eco RI bevatten, worden geïdentificeerd en gepoold. De totale en specifieke activiteit worden berekend. Aanbevolen voor cursussen voor gevorderden.