"STRATAGENE"

Supplier: Thermo Fisher Scientific

Piko® PCR plates utilise ultra-thin wall (UTW) technology to dramatically reduce the thickness of the well walls, and are specifically designed for use in the Piko® thermal cycler and the PikoReal® Real Time PCR system. Piko® PCR plates have the footprint of a microscope slide. Four Piko® 24-well (96-well) plates can be snapped into the plate frame to create a standard 96-well (384-well) plate.

Supplier: AGILENT

The pBluescript II phagemids (plasmids with a phage origin) are cloning vectors designed to simplify commonly used cloning and sequencing procedures, including the construction of nested deletions for DNA sequencing, generation of RNA transcripts in vitro and site-specific mutagenesis and gene mapping.

Supplier: AAT Bioquest

TAQuest™ qPCR Master mix for TaqMan probes is a ready-to-use 2X solution optimised for qPCR and two-step RT-qPCR compatible with TaqMan gene expression assays.

Supplier: EPPENDORF

Eppendorf twin.tec® PCR plates LoBind, available in 96 and 384-well formats, maximize nucleic acid recovery and are certified as 'PCR clean' for consistently reliable results.

Supplier: AGILENT

Pfu DNA ligase is a recombinant enzyme derived from Pyrococcus furiosus hyperthermophilic marine archaebacterium that catalyses linkage of adjacent 5'-phosphate and 3'-hydroxy ends of double-stranded DNA at 45 to 80 °C, with a temperature optimum near 70 °C for nick-sealing reactions.

Supplier: AAT Bioquest

TAQuest™ qPCR Master mix for TaqMan probes is a ready-to-use 2X solution optimised for qPCR and two-step RT-qPCR compatible with TaqMan gene expression assays.

Supplier: AAT Bioquest

TAQuest™ qPCR Master mix for TaqMan probes is a ready-to-use 2X solution optimised for qPCR and two-step RT-qPCR compatible with TaqMan gene expression assays.

Supplier: AAT Bioquest

TAQuest™ FAST qPCR Master mix for TaqMan probes is a ready-to-use 2X solution optimised for qPCR and two-step RT-qPCR well suited to use for TaqMan gene expression assays.

Supplier: AGILENT

Protein directed evolution in just one step.

Supplier: EPPENDORF

PP-wells met PC-behuizing en skirt. Door de robuustheid van de plaat is hij ideaal voor verwerking met robots en de PP-wells hebben een inert, niet nat wordend oppervlak waar geen DNA, RNA en enzymen op kunnen binden. De ultradunne wanden zorgen voor optimale warmteoverdracht naar het monster. Alle platen zijn autoclaveerbaar en gecertificeerd vrij van RNase, DNase of menselijk DNA.

Supplier: Quantabio

PerfeCTa® qPCR SuperMixes zijn 2X geconcentreerde, gebruiksklare reactiecocktails die alle componenten bevatten behalve primers, probes en template voor qPCR.

Supplier: AAT Bioquest

TAQuest™ FAST qPCR Master mix for TaqMan probes is a ready-to-use 2X solution optimised for qPCR and two-step RT-qPCR well suited to use for TaqMan gene expression assays.

Supplier: Quantabio

Ultra-Plex™ 1-Step ToughMix® is a ready-to-use, 4X-concentrated master mix for reverse transcription quantitative PCR (RT-qPCR) of RNA templates using hybridisation probe detection chemistries such as TaqMan® 5’-hydrolysis probes on Real-Time PCR systems that utilise ROX passive reference with 490 nM excitation. First-strand cDNA synthesis and PCR amplification are carried out in the same tube without opening between procedures. It is ideal for highly sensitive quantification of RNA viruses or low abundance RNA targets in uni- or multiplexed RT-qPCR applications as well as high throughput gene-expression studies. The system has been optimised to deliver maximum RT-qPCR efficiency, sensitivity, and specificity in reduced reaction volumes and fast cycle times. Ultra-Plex™ 1-Step ToughMix® contains all required components for RT-qPCR except RNA template and probe. It is compatible with all dual-labeled probe chemistries.

Supplier: AAT Bioquest

TAQuest™ FAST qPCR Master mix for TaqMan probes is a ready-to-use 2X solution optimised for qPCR and two-step RT-qPCR well suited to use for TaqMan gene expression assays.

Supplier: Quantabio

Deze FastMixes bevatten buffers en stabilisatoren die zijn geoptimaliseerd voor een maximale efficiëntie, reproduceerbaarheid en gevoeligheid van PCR met TaqMan®- en TaqMan® MGB-probechemie. Een belangrijke component van PerfeCTa® qPCR FastMixes is AccuFast™ Taq DNA-polymerase, die directe activering en snelle polymerisatiekinetiek mogelijk maakt. Bij activatie door verhitting (20 seconden bij 95 °C) worden de antilichamen onomkeerbaar gedenatureerd, waarbij volledig actieve, ongemodificeerde Taq DNA polymerase wordt vrijgegeven. Hierdoor wordt specifieke en efficiënte primeruitbreiding gestimuleerd met als voordeel de reactie-initiatie bij kamertemperatuur. FastMixes leveren maximale efficiëntie, gevoeligheid en specificiteit voor PCR en een robuust fluorescentiesignaal met snelle of conventionele cyclercondities.

Supplier: AGILENT

For libraries constructed from highly methylated DNA, Gigapack III Packaging Extract simplifies the packaging procedure and increases the efficiency and representation of libraries constructed from highly methylated DNA. Each packaging extract is restriction minus to optimize packaging efficiency and library representation.