1051 Resultaten voor: "SIGMA ALDRICH MICROSCOPY"

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7-Diethylamino-3-(4-maleimidophenyl)-4-methylcoumarin has been used: as a nontoxic substitute for conjugation with antibody(93) to aid fluorescent detection of Coenzyme A (CoA-SH) in human N-myristoyltransferases assay (94)

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Phloroglucinol (phlo) is a phenol derivative that shows a cytoprotective effect from oxidative damage by enhancing the activity of cellular catalase. It can also be used to prepare synthetic analogs of A-type proanthocyanidins (PACs) such as 2,8-dioxabicyclo nonane derivatives by reacting with the corresponding flavylium salts. Phlo can react with benzaldehyde derivatives to form phloroglucinol-based microporous polymeric organic frameworks (phlo-POF) with potential applications in ion exchange and gas adsorption. Phloroglucinol is an antispasmodic and a derivative of phenol.

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Used as a red nuclear counterstain in various cytochemical procedures.

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Protargol silver impregnation and neuronal selectivity for light and electron microscopic observations.

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Nile blue chloride has been used to stain non-aqueous phase in fluorescence microscopy studies.

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Azure A eosinate, powder, crystals or chunks, Sigma-Aldrich®

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Percoll® is a classic medium for density gradient centrifugation of cells, viruses, and subcellular particles. It consists of colloidal silica particles of 15 – 30 nm diameter (23% w/w in water), coated with polyvinylpyrrolidone (PVP).

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Rhodamine 123 is useful as a laser dye and to have selective cell growth effects. It has also been used to measure mitochondrial membrane potential.

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Fast Red Violet LB Salt has been used for the detection of tartrate-resistant acid phosphatase (TRAP) in tissue sections.

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Tetrazolium Violet is a monotetrazolium salt which forms water-insoluble formazans. These salts are mainly used as indicators of extracellular redox activity and cell redox potential. Tetrazolium violet is positively charged and cell-permeable.

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Phenol Red sodium salt is widely used as a pH indicator. At a pH of 6,4 and below, phenol red solution will have a yellow colour. At a pH of 8,2 and above, it will have a red colour. It is commonly used in culture media to detect neutral to acidic pH changes.

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2,4-Dimethoxytoluene, Sigma-Aldrich®

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3-Ethyl-2-methylbenzothiazolium iodide, Sigma-Aldrich®

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Dihydrorhodamine 123 has been used for the detection of free intracellular radicals or ROS (reactive oxygen species).

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The combination of azure A and silver staining is used for the quantification of glycosaminoglycans in agarose gels. It is also used for the staining of chromosomes in the nucleus. Azure A is a main component of the azure B/polychromed methylene blue-eosin stains family, also referred to as the Romanowsky stain. Azure A is a basic cationic dye.

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Osmium tetroxide is also referred to as osmium and is mainly used as a secondary fixative of tissues. The molecule reacts with lipid moieties and is responsible for the oxidation of unsaturated bonds of fatty acids. This reaction adds density and contrast to biological samples. In normal tissues, presence of osmium results in intense black staining which can be viewed by normal eye. In addition, it can be used as a mordant (a molecule which combines with stain or dye at later stages) in electron microscopy, thereby enhancing lead staining.

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Gentian violet is used in the Gram staining for distinguishing between gram-positive and gram-negative organisms.

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6-Aminofluorescein has been demonstrated to be useful fluorescent labeling reagent for fullerene-based liposome nanostructures termed 'buckysomes'. 6-aminofluorescein dissolved in 0,1 N NaOH can be quantified from a maximum absorbance at 490 nm. Decarboxylated 6-aminofluorescein dissolved 6 N HCl (prepare by constant boiling at 110 °C for 24 h) can be quantified from a maximum absorbance at 454 nm. Aldehyde groups are formed by oxidateive damage to proteins, which can be detected by conjugation to decarboxylated 6-aminofluorescein. 6-Aminofluorescein has been used to determine its ability to potentiate the antiviral activity of poly r (A-U).

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Crystal violet has been used to determine cell viability. It has been used in the staining of cells for the quantification of cellular migration.

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Fluoresceinamine, isomer I has been used as a probe in immobilization and is used to determine its ability to potentiate the antiviral activity of poly r (A-U).

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Useful for staining DNA, chromosomes and nuclei. May be used for fluorescence microscopy or flow cytometry.
Excitation max. = 346 nm
Emission max. = 460 nm
bisBenzimide H 33342 trihydrochloride has been used for the staining of viable and apoptotic.

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1-Methoxy-5-methylphenazinium methyl sulfate (MPMS) is a photochemically stable electron-transport mediator between NAD(P)H and tetrazolium dyes. The reduced form of MPMS converts tetrazolium salt to purple precipitate formazan. It is a very stable and non-enzymic electron carrier that mediates electron transfer by dehydrogenases in vitro to various electron acceptors such as tetrazolium dyes or the electrode of an enzymic electric cell.

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4,5-Methylenedioxy-1,2-phenylenediamine dihydrochloride is a fluorescence tag for α keto acids. It is also required for the derivatisation of sialic acids in presence of dilute sulfuric acid. The method allows determination of sialic acids.

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Rose Bengal has been used to evaluate acrosome integrity. It has also been used to initiate photothrombotic occlusion in blood.

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As a direct dye, Direct Yellow 27 is an acid dye with large molecules that will bind to cellulose fibers directly. It is mono azo dye with good light fastness.

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Certified for Giemsa stain for blood films and for the Wolbach′s Giemsa method for paraffin sections. Giemsa is a versatile polychromatic stain, which is suitable for staining a diverse range of specimens. It is a buffered thiazine-eosinate solution designed to provide coloration of blood cells. Giemsa’s stain is frequently used for diagnostic purposes in hematology to differentiate the nuclear and cytoplasmic morphology of platelets, RBCs, WBCs, and parasites. It is frequently used in combination with other dye solutions as May-Grunwald’s solution for Pappenheim (MGG) and Wright-Giemsa. The Giemsa stain was adapted to histology due to its unique staining of chromatin, nuclear membranes, and cytoplasmic elements. Giemsa′s staining solution is composed of methylene blue, azure, and eosin.

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Pyronin G, Sigma-Aldrich®

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Fluorescent Brightener (Calcofluor White M2R) is used for the staining of fungi and as a viability stain. Industrial uses include use as a fluorescent brightening agent for cellulose and polyamide fabrics, paper and in detergents and soaps.

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1,1'-Dioctadecyl-4,4'-bipyridinium dibromide,97% (Octadecyl viologen dibromide)

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Crystal Ponceau 6R has been used in the MSB (martius yellow–crystal scarlet–methyl blue) staining procedure for the staining of collagen fibers and erythrocytes.