"Quantabio"
PerfeCTa® qPCR ToughMix®
Supplier: Quantabio
PerfeCTa® qPCR ToughMix® is een 2X geconcentreerde, gebruiksklare reactiecocktail voor PCR-amplificatie van DNA-templates en lost veel bekende PCR-remmers op die vaak aanwezig zijn in ruwe monsters geëxtraheerd uit milieumonsters, plantaardig weefsel of dierlijk weefsel. Het is een veelzijdig en robuuste realtime qPCR-reagens die zorgt voor maximale gevoeligheid en PCR-efficiëntie bij uiteenlopende fluorogene sondechemie, zoals TaqMan® hydrolyseprobes. PerfeCTa® qPCR ToughMix® bevat alle benodigde reactiebestanddelen, behalve primers, probe(s), en DNA-templates. De lichtblauwe kleur van de AccuVue™ tracerkleurstof vereenvoudigt reactievorming in witte of heldere platen en draagt bij aan minimale pipetteer- of mengfouten. Het heeft geen invloed op de qPCR-prestaties of de stabiliteit van het product.
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sparQ PureMag beads
Supplier: Quantabio
sparQ PureMag beads is a fast and reliable nucleic acid purification system for reaction cleanup and size selection in Next Generation Sequencing (NGS) workflows. Based on the reversible nucleic acid-binding properties of magnetic beads; this product can be used to quickly remove primers, primer-dimers, unincorporated nucleotides, salts, adapters and adapter-dimers from NGS library prep reactions to improve downstream sequencing performance. sparQ PureMag beads allows excellent recovery of fragments greater than 100 bp without centrifugation or filtration. Consistent and reliable size selection can be achieved by simply adjusting the beads to sample ratio. This product is designed for both manual and automated processing, allowing seamless integration into existing workflows.
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AccuStart™ II GelTrack PCR SuperMix
Supplier: Quantabio
AccuStart™ II GelTrack PCR SuperMix is a 2X concentrated, ready-to-use reaction cocktail for routine PCR amplification of DNA fragments up to 4 kb followed by analysis on agarose gels. It contains all components, except primers and template. AccuStart™ II GelTrack PCR SuperMix simplifies reaction assembly, improves assay reproducibility, and reduces the risk of contamination. The supermix includes electrophoresis tracking dyes that migrate at approximately 4 kb and 50 bp to allow direct loading of PCR product on agarose gels following amplification. AccuStart™ II Taq DNA polymerase in the master mix is inactivated with monoclonal antibodies that bind the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (1 minute at 94 °C), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly.
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cDNA synthesis, qScript™ Ultra SuperMix
Supplier: Quantabio
Faster, better, tougher next generation cDNA SuperMix for cDNA synthesis.
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qPCR SuperMixes, PerfeCTa®
Supplier: Quantabio
PerfeCTa® qPCR SuperMixes zijn 2X geconcentreerde, gebruiksklare reactiecocktails die alle componenten bevatten behalve primers, probes en template voor qPCR.
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AccuStart™ II Taq DNA polymerase
Supplier: Quantabio
AccuStart™ II Taq DNA Polymerase is a high purity, recombinant Taq DNA polymerase preparation with high avidity monoclonal antibodies that bind the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (1 minute at 94 °C), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly. Non-specific extension of primers at low temperatures is a common cause of artifacts and poor sensitivity in PCR. The AccuStart™ II automatic hot-start enables specific and efficient primer extension in the PCR process with the added convenience of room temperature reaction assembly. The included 10X PCR Buffer II is a new optimised buffer that provides higher product yield, improved specificity, and enhanced multiplexing capability.
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AccuStart™ Genotyping ToughMix®
Supplier: Quantabio
AccuStart™ Genotyping ToughMix® is a 2X concentrated ready to use reaction cocktail for PCR amplification of DNA templates that overcomes inhibitors often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues. It is a versatile and robust PCR reagent that provides highly specific and efficient amplification for genotyping sequence variants including allelic discrimination of single nucleotide polymorphisms (SNPs) using a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes.
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sparQ RNA-Seq HMR kits
Supplier: Quantabio
Ultra FAST RNA library prep with integrated rRNA and globin depletion.
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Extended warranty for Q cycler
Supplier: Quantabio
Q Instrument extended warranty program.
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Extracta Plus RNA kits
Supplier: Quantabio
Rapid extraction and purification of high-quality total RNA from cultured cells or tissue.
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SparQ Lysis Kits
Supplier: Quantabio
High-quality, high molecular weight gDNA extraction from DBS for high-throughput workflows.
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PerfeCTa® MultiPlex qPCR SuperMix, low ROX
Supplier: Quantabio
PerfeCTa® Multiplex qPCR SuperMix, low ROX is a 2X concentrated, ready to use reaction cocktail that contains all components, except primers, probe(s), and template for real time qPCR on Applied Biosystems 7500 or Stratagene MX series of real time PCR systems.
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qScript™ One-Step qRT-PCR kits
Supplier: Quantabio
Synthesis of cDNA and PCR amplification are carried out in the same tube without opening between procedures. These kits are optimised to deliver maximum RT-PCR efficiency and specificity, enabling unbiased co-amplification of low copy transcripts in the presence of higher copy reference genes. As a result the qScript™ One-Step qRT-PCR Kits offer improved sensitivity over other commercially available qPCR reagents. The qScript™ One-Step Master Mix maximises the activities of both reverse transcriptase and PCR enzymes, while minimising primer-dimer formation. High specificity is assured with AccuStart™ Taq DNA Polymerase, which is bound by monoclonal antibodies for superior hot-start. Upon heating to 95 °C, the antibodies denature irreversibly releasing fully active and unmodified Taq DNA polymerase. qScript™ One-Step qRT-PCR Kits are available for all Real-Time PCR instrument platforms, including those requiring normalisation with ROX reference dye.
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Virus detection kit, qScript™ 1-Step Virus ToughMix®
Supplier: Quantabio
Superior sensitivity for viral RNA detection.
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PerfeCTa® qPCR FastMix® II
Supplier: Quantabio
PerfeCTa® qPCR FastMix® II is an advanced qPCR reagent system for both fast and conventional PCR cycling protocols or instruments. It is a versatile and robust solution that provides the ultimate sensitivity and high PCR efficiency using a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes. PerfeCTa® qPCR FastMix® II is provided as a 2X concentrated ready to use reaction cocktail that contains all required reaction components, except primers, probe(s), and DNA template. The light blue colour of the AccuVue™ tracer dye simplifies reaction assembly in white, or clear, plates and helps to minimise pipetting or mixing errors. It does not interfere with qPCR performance or affect the stability of the product.
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One Step Fast qRT PCR-kits, qScript™
Supplier: Quantabio
Deze kits bieden een eenvoudige en zeer gevoelige oplossing voor realtime reverse transcriptase-PCR (qRT-PCR) van RNA-templates door gebruik te maken van detectiechemie met hybridisatieprobes, zoals TaqMan® 5'-hydrolyseprobes.
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