"PCR Mastermixes"

Supplier: Quantabio

Synthesis of cDNA and PCR amplification are carried out in the same tube without opening between procedures. These kits are optimised to deliver maximum RT-PCR efficiency and specificity, enabling unbiased co-amplification of low copy transcripts in the presence of higher copy reference genes. As a result the qScript™ One-Step qRT-PCR Kits offer improved sensitivity over other commercially available qPCR reagents. The qScript™ One-Step Master Mix maximises the activities of both reverse transcriptase and PCR enzymes, while minimising primer-dimer formation. High specificity is assured with AccuStart™ Taq DNA Polymerase, which is bound by monoclonal antibodies for superior hot-start. Upon heating to 95 °C, the antibodies denature irreversibly releasing fully active and unmodified Taq DNA polymerase. qScript™ One-Step qRT-PCR Kits are available for all Real-Time PCR instrument platforms, including those requiring normalisation with ROX reference dye.

Supplier: Quantabio

Ultra-Plex™ 1-Step ToughMix® is a ready-to-use, 4X-concentrated master mix for reverse transcription quantitative PCR (RT-qPCR) of RNA templates using hybridisation probe detection chemistries such as TaqMan® 5’-hydrolysis probes on Real-Time PCR systems that utilise ROX passive reference with 490 nM excitation. First-strand cDNA synthesis and PCR amplification are carried out in the same tube without opening between procedures. It is ideal for highly sensitive quantification of RNA viruses or low abundance RNA targets in uni- or multiplexed RT-qPCR applications as well as high throughput gene-expression studies. The system has been optimised to deliver maximum RT-qPCR efficiency, sensitivity, and specificity in reduced reaction volumes and fast cycle times. Ultra-Plex™ 1-Step ToughMix® contains all required components for RT-qPCR except RNA template and probe. It is compatible with all dual-labeled probe chemistries.

Supplier: MP Biomedicals

Taq-&GO™ is a ready to use optimised reaction mix for PCR.

Supplier: Thermo Fisher Scientific

Het Verso™ qRT-PCR systeem combineert het Verso™ RT-enzym met ABsolute™ Blue qPCR Master Mix voor maximaal gemak en optimale veelzijdigheid. Verkrijgbaar in formaat met 1 en 2 stappen, alsmede met meerdere verpakkingsgrootten, om te kunnen voldoen aan alle toepassings- en doorvoereisen.

Supplier: VWR Chemicals

VWR® Taq Plus is an optimised format of Taq DNA polymerase master mix and, therefore, is a good alternative to Taq DNA polymerase and Taq DNA polymerase master mix.

Supplier: Thermo Fisher Scientific

The Maxima™ SYBR® Green qPCR Master Mix (2X) is a ready to use solution optimised for quantitative real time PCR and two-step real time RT-PCR on most real time PCR machines. The master mix includes Maxima™ hot start Taq DNA polymerase, SYBR® Green dye I and dNTPs in an optimised PCR buffer. ROX solution is provided in a separate vial for use with machines that require ROX as a passive reference dye. Maxima™ hot start Taq DNA polymerase in combination with the optimised buffer ensures PCR specificity and sensitivity. The SYBR® Green I intercalating dye allows for DNA detection and analysis without using sequence specific probes. dUTP is included in the mix for optional carryover contamination control using uracil-DNA glycosylase (UDG). The use of Maxima™ SYBR® Green qPCR Master Mix (2X) in real time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates.

Supplier: PRIMERDESIGN

These high quality assays detect the SARS-CoV-2 variants of concern to give fast and clear results to customers.

Supplier: FINNZYMES REAGENTS

Phusion® High-Fidelity PCR Master Mix with HF Buffer is a convenient 2X mix containing Phusion® DNA Polymerase, nucleotides and optimised HF buffer including MgCl₂. Only template and primers need to be added by the user.

Supplier: Thermo Fisher Scientific

PCR Master Mix is a 2X concentrated solution of Taq DNA polymerase, dNTPs and all other components required for PCR, except DNA template and primers. This pre-mixed formulation saves time and reduces contamination due to a reduced number of pipetting steps required for PCR set up. The mix is optimised for efficient and reproducible PCR.

Supplier: VWR Chemicals

Fast HiFi DNA Polymerase 2 U/µl is een DNA-polymerase met proefleesfunctie dat de volgende eigenschappen vertoont: hoge nauwkeurigheid (>60x Taq DNA-polymerase), het vermogen om problematische DNA-targets te amplificeren, zoals targets met een laag tot hoog GC-gehalte, en het vermogen om lange DNA-targets te amplificeren. Het wordt aanbevolen voor toepassingen die een extreem hoge nauwkeurigheid vereisen, zoals klonen/subkloneren, NGS-toepassingen en mutagenese.

Supplier: Stemcell Technologies

For probe-based assays and arrays.

Supplier: VWR Chemicals

Fast HiFi Hot Start DNA Polymerase 2X Master Mix is a convenient alternative to Fast HiFi Hot Start DNA Polymerase, displaying the same set of features. Fast HiFi Hot Start DNA Polymerase 2X Master Mix is also available with a red dye for direct gel loading.

Supplier: EPPENDORF

Hoge kwaliteit polypropyleen, transparant.

Supplier: Quantabio

sparQ Universal Library Quant Kit provides rapid and accurate quantification of libraries prepared for sequencing on Illumina® NGS platforms.

Supplier: EPPENDORF

Eppendorf Tubes® with snap caps are an ideal choice when working with medium-sized sample volumes (0,5 to 5,0 ml). Designed for user-friendly handling, broad temperature ranges, centrifugation safety (up to 25000×g), and sample recovery.

Supplier: OMEGA BIO-TEK

High-throughput DNA- en RNA-isolatie uit gekweekte cellen en weefsel met behulp van magnetische kralen.