"PCR Mastermixes"

Supplier: Enzo Life Sciences

Rapid removal of contaminating DNA in PCR mastermixes, without reduction in PCR sensitivity.

Supplier: MP Biomedicals

Taq-&LOAD™ PCR Master Mix is an optimised 5X reaction mix for PCR containing recombinant Taq DNA polymerase, dNTPs, MgCl₂, and incubation and loading buffers. Also included are a compound increasing the density of the sample and a red/purple dye.

Supplier: VWR Chemicals

VWR® Red Taq DNA Polymerase Master Mix, which also contains an inert red dye, can be directly loaded onto an agarose gel without addition of electrophoresis loading buffers.

Supplier: VWR Chemicals

VWR® TEMPase Hot Start DNA Polymerases are highly stable polymerases, featuring higher specificity, superior sensitivity and greater yields compared to standard DNA polymerases. These features make them well suited for the detection of low abundance targets. Other uses include screening, amplification of GC-rich sequences, multiplex PCR, direct PCR and qPCR.

Supplier: VWR Chemicals

VWR® Taq DNA Polymerase Master Mix is a ready to use 1,1X or 2X reaction mix. Simply add primers, template and water to carry out primer extensions and other molecular biology applications.

Supplier: VWR Chemicals

TEMPase Hot Start DNA Polymerase Master Mix and Blue TEMPase Master Mix are good alternatives to TEMPase Hot Start DNA Polymerase. These master mixes offer easy reaction assembly at room temperature, reduced set-up time and fewer handling steps, which lead to increased reproducibility. As a consequence TEMPase Hot Start DNA Polymerase Master Mix is highly suited to standard tests.

Supplier: G-Biosciences

Pfu DNA Polymerase, derived from the hyperthermophilic archae Pyrococcus furiosus, has superior thermostability and proofreading properties compared to other thermostable polymerases. It has a molecular weight of 90 kDa and can amplify DNA targets up to 2 kb. The elongation velocity is 0,2~0,4 kb/min (70~75 °C). Pfu DNA Polymerase possesses 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide misincorporation errors. This means that Pfu DNA Polymerase-generated PCR fragments will have fewer errors than Taq-generated PCR inserts. Using Pfu DNA Polymerase results in blunt-ended PCR products, which are ideal for cloning into blunt-ended vectors. Pfu DNA Polymerase is superior for techniques that require high-fidelity DNA synthesis.

Supplier: G-Biosciences

Taq DNA Polymerase is a highly thermostable recombinant DNA polymerase derived from the thermophile Thermus aquaticus. The molecular weight of the recombinant protein is 94 kD. The Taq Polymerase is able to amplify DNA up to 5 kb with an elongation velocity of 0,9 to 1,2 kb/min at 70 to 75 °C. The error rate of this Taq Polymerase is ~2,2×10⁻⁵ nucleotide⁻¹ cycle⁻¹. Taq DNA polymerase catalyses the 5’→3’ synthesis of DNA. The enzyme has no detectable 3’→5’ proofreading exonuclease activity, and possesses low 5’→3’ exonuclease activity, which results in a 3’-dA overhang on the PCR product

Supplier: Biotium

A qPCR master mix containing Evagreen® qPCR dye and Cheetah™ Taq hotstart DNA polymerase, suitable for qPCR using a fast cycling protocol.

Supplier: VWR Chemicals

Fast HiFi DNA Polymerase 2 U/µl is a proofreading DNA polymerase displaying the following features; high fidelity >60X Taq DNA Polymerase, ability to amplify problematic DNA targets, such as those with low to high GC content and ability to perform amplification on long DNA targets. lt is recommended for applications, which require extremely high fidelity such as cloning / sub cloning, NGS applications and mutagenesis.

Supplier: PRIMERDESIGN

The oasig freeze drying process stabilises all of the active components allowing these reagents to be shipped and stored at room temperature. This hugely simplifies the logistics of purchasing, shipping and using the technology. Whether you are in a sophisticated laboratory in Texas or a mobile field hospital in Timbuktu we can supply complete qPCR kit and reagent packages to your door quickly and cheaply via standard shipping methods without the need for dry ice or a cold chain of any sort.
Freeze dried qPCR reagents stored and shipped at ambient temperatures:

Supplier: Quantabio

qScript™ XLT One-Step RT-qPCR ToughMix® is een gebruiksklare, zeer gevoelige formule voor kwantitatieve omgekeerde transcriptie-PCR (RT-qPCR) van RNA-templates.

Supplier: AGILENT

The Brilliant HRM Ultra-Fast Loci Master Mix for High Resolution Melt (HRM) analysis.

Supplier: Merck Millipore (Novagen)

De NovaTaq PCR Master Mix is een kant-en-klaar, 2X geconcentreerd mengsel van NovaTaq DNA-polymerase, ultrapure deoxynucleotiden en reactiebuffer zonder magnesiumchloride. De Master Mix vereenvoudigt de vorming van PCR-reacties, en biedt als voordelen tijdbesparing, consistentie en minimale kans op besmetting. Voeg gewoon NovaTaq PCR Master Mix toe aan hetzelfde volume met de vereiste hoeveelheid magnesiumchloride, DNA-sjabloon en primers, en de reactie is klaar voor thermocycling. De uiteindelijke verdunde reactie bevat 2,5 eenheden NovaTaq DNA polymerase per 100 µl. Er worden voldoende componenten meegeleverd voor 200 standaard amplificatiereacties van 50 µl (of 100×100 µl).

Supplier: Thermo Fisher Scientific

ABsolute Blue™ Master Mix bevat een inerte blauwe kleurstof om het contrast tussen reagens en kunststof aanzienlijk te vergroten, waardoor de verificatie van Master Mix-doseringen snel, gemakkelijk en zonder problemen kan worden uitgevoerd. Toepassingen zijn onder meer genexpressie, siRNA-validatie, bepaling van SNP-type en genotype.

Supplier: Quantabio

Deze SuperMix is een 2x geconcentreerde, gebruiksklare reactiecocktail voor realtime qPCR die alle componenten bevat behalve primers, probes en DNA templates. Het systeem overstijgt de multiplexbeperkingen van conventionele PCR-mengsels, waardoor zuivere amplificatie van maximale vijf targetsequenties in één buisje mogelijk is. Onderdrukking van ampliconen met een beperkt aantal kopieën door referentietargets met veel kopieën in de amplificatie is een veel voorkomend probleem bij multiplex-PCR. Hierdoor kan de schijnbare representatie en kwantificering van doelsequenties met een beperkt aantal kopieën worden vertekend of gemaskeerd. PerfeCTa® MultiPlex qPCR SuperMix geeft een dynamisch bereik en gevoeligheid aan multiplex-qPCR die vergelijkbaar zijn met die voor probe-analyses van enkelvoudig qPCR, zonder dat er beperkende of variabele primerconcentraties nodig zijn.