"Cytiva"

Supplier: Cytiva

Purge tubing kit

Supplier: Cytiva

Staphylococcal protein A is the classical immunoglobulin-binding reagent for a wide range of antibodies, used extensively in affinity chromatography and antibody detection applications. The protein is enterotoxin-free and gives reliable results. Protein A binds the Fc regions of IgG antibodies from a variety of species, for convenient antibody purification.

Supplier: Cytiva

Sepharose™ 4B is an agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling.

Supplier: Cytiva

HiTrap™ Butyl FF are prepacked Butyl Sepharose™ 4 Fast Flow columns for hydrophobic interaction chromatography (HIC).

Supplier: Cytiva

HiTrap™ SP FF is pre-packed with SP Sepharose™ Fast Flow, and is a strong cation exchanger for small scale protein purifications as well as screening of binding and elutions conditions.

Supplier: Cytiva

SP Sepharose™ High Performance is composed of crosslinked agarose beads with a mean diameter of 34 µm, modified with sulphopropyl (SP) strong cation exchange groups.

Supplier: Cytiva

Octyl Sepharose™ 4 Fast Flow resin is based on cross-linked 4% agarose. The aliphatic ligand is immobilised to the base matrix with an ether linkage. The ligand contains no charged groups, making true hydrophobic interaction chromatography possible, without interfering ionic effects.

Supplier: Cytiva

SOURCE™ 15Q PE is a strong anion exchanger prepacked in a tricorn column for intermediate lab-scale purification and large-scale polishing of biomolecules with ion exchange chromatography.

Supplier: Cytiva

Sephadex™ G-15 is a well established gel filtration medium for desalting and buffer exchange of peptides and small biomolecules >1500 molecular weight.

Supplier: Cytiva

Toepassing: FPLC-kolom, HiTrap™, HiTrap™ Fast Flow IEX Selection Kit, Bedafmetingen: 16×25 mm, Inhoud: 5 ml

Supplier: Cytiva

This protein A column is prepacked with MabSelect SuRe LX affinity resin and is part of our process development platform. These packed columns are an excellent choice for method optimization and parameter screening for capture of monoclonal antibodies when a high dynamic binding capacity is needed.

Supplier: Cytiva

For use in ELISA systems. For ELISA, it is recommended that the conjugate should be diluted in the range 1:4000 to 1:8000.

Supplier: Cytiva

His SpinTrap is a prepacked, single-use spin column for purifying histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

Supplier: Cytiva

CyDye™ DIGE fluors are available as minimal and saturation labeling dyes. The minimal dyes are intended for general 2-D application use where sufficient amounts of sample are available. The saturation dyes from the Scarce Sample Labeling Kit are designed to be used for applications where only small amounts of sample are available, for example in Laser Capture Microdissection.

Supplier: Cytiva

Amersham CyDye NIR-labeled secondary antibodies enable quantitation of Western blots with a strong signal-to-noise ratio. Mulitplexed experiments allow for the detection of two proteins on a single blot. The use of total lane signals to normalize target protein data yield robust and reliable results.

Supplier: Cytiva

Amersham ECL™ DualVue™-markers voor Western blotting voeren twee belangrijke functies uit. Ten eerste bevestigen de vooraf gekleurde proteïnemarkers de proteïneoverdracht en wordt de blottingoriëntatie duidelijk gedefinieerd. Ten tweede worden recombinante, gelabelde proteïnen gedetecteerd op film of via CCD-beeldvorming in combinatie met de doelproteïne(n), waardoor een zeer nauwkeurige bepaling van de molecuulmassa van de doelproteïne(n) mogelijk is. De gelabelde markers kunnen eenvoudig worden gedetecteerd, met name door een specifiek conjugaat dat voorkomt dat er kruisbesmetting tussen doelproteïne(n) en markers optreedt.