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28226 Resultaten voor: "Biotium"

Gelkleurstoffen voor nucleïnezuren, GelRed™ en GelGreen™

Gelkleurstoffen voor nucleïnezuren, GelRed™ en GelGreen™

Supplier: Biotium

GelRed and GelGreen are highly sensitive DNA/RNA gel stains made safe by preventing penetration into cells.

    
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EvaGreen® Plus dye, 20X in Water

Supplier: Biotium

EvaGreen® Plus Dye has an improved signal-to-noise compared to original EvaGreen® dye, for higher sensitivity in DNA amplification applications.

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PMA™, PMAxx™, Real-Time PCR Viability Kit

PMA™, PMAxx™, Real-Time PCR Viability Kit

Supplier: Biotium

Viability PCR With PMA and PMAxx™ dyes

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One-Step Blue® protein gel stain

One-Step Blue® protein gel stain

Supplier: Biotium

One-Step Blue® is a ready-to-use protein gel staining solution. It produces fast (5-60 min) protein staining in a single step without fixation or washing.

    
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Wheat germ agglutinin (WGA) conjugates

Wheat germ agglutinin (WGA) conjugates

Supplier: Biotium

A wheat germ agglutinin (WGA) lectin labelled with Biotium’s CF® Dyes or HRP. It is a carbohydrate-binding lectin that has high affinity for sialic acid and N-acetylglucosamine moieties of glycoproteins. As a result, WGA conjugates will label yeast bud scars and the cell membrane of gram-positive bacteria and mammalian cells.

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Fluorescence antifade mounting media and CoverGrip™ coverslip sealant, EverBrite™

Fluorescence antifade mounting media and CoverGrip™ coverslip sealant, EverBrite™

Supplier: Biotium

EverBrite™ mounting media are anti-fade mountants for preserving fluorescence during microscopy. EverBrite™ mounting media prevent rapid photobleaching of fluorescein, rhodamine, and cyanine based dyes and are optimally formulated for use with Biotum’s CF™ dye conjugates. Unlike Vectashield® EverBrite™ mounting media is compatible with Cy® dyes such as Cy®2, Cy®3 and Cy®5. EverBrite™ mounting medium is a wet-set medium while EverBrite™ hardset mounting medium cures overnight to form a hard permanent seal with the coverslip, eliminating the need to seal coverslip edges with nail polish or other sealants. Both formulations are available with DAPI for blue fluorescent nuclear counterstaining without an additional staining step.

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Nucleic acid stains, PAGE GelRed™

Nucleic acid stains, PAGE GelRed™

Supplier: Biotium

Stains specifically designed for DNA in polyacrylamide gels.

    
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Dimethylsulfoxide, watervrij ≥99.9%

Supplier: Biotium

Anhydrous DMSO (dimethyl sulfoxide; methyl sulfoxide) is recommended for preparing stock solutions.

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GelRed® Prestain Plus 6X DNA loading dye

GelRed® Prestain Plus 6X DNA loading dye

Supplier: Biotium

6X DNA loading buffer that includes ultra-sensitive, non-toxic GelRed® dye.

    
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Fast probe master mixes

Fast probe master mixes

Supplier: Biotium

A qPCR master mix containing Cheetah™ Taq hotstart DNA polymerase, suitable for probe-based real-time PCR applications.

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PMAxx™ dye 20 mM in water

PMAxx™ dye 20 mM in water

Supplier: Biotium

PMAxx™ dye is a DNA modifier used for viability PCR (v-PCR). V-PCR is a powerful PCR-based, culture-independent, method for rapid and sensitive quantification of microbial viability.

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Super HT PAP pens

Super HT PAP pens

Supplier: Biotium

The Super HT PAP pen draws a water repellent circle around slide mounted tissue and prevents the waste of valuable reagents by keeping the liquid pooled in a single droplet. The regular sized super HT PAP pen can be used in about 800 applications, while the mini sized super HT PAP Pen will last about 400 applications.

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SYBR® Green I 10000x in DMSO nucleic acid gel stain

Supplier: Biotium

Thiazole green, which is structurally identical to SYBR® green I nucleic acid gel stain, is one of the most sensitive stains available for detecting double-stranded DNA (dsDNA) in agarose, polyacrylamide gels and qPCR.

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Propidium iodide 1 mg/ml in water

Propidium iodide 1 mg/ml in water

Supplier: Biotium

Propidium iodide (PI) is a membrane-impermeant nucleic acid intercalator. The dye is commonly used to selectively stain dead cells in a cell population and also used as a counterstain in multicolor fluorescent imaging.

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6X GelRed prestain loading buffer with tracking dye

Supplier: Biotium

6X GelRed prestain loading buffers are gel loading buffers containing density agents, tracking dyes, and GelRed dye. GelRed is a sensitive, stable and environmentally safe fluorescent nucleic acid dyes designed to replace the highly toxic ethidium bromide (EtBr).

    
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Fluorometric polymerase activity assay kit, EvaEZ™

Supplier: Biotium

EvaEZ™ Fluorometric Polymerase Activity Assay Kit provides an easy and accurate way to determine the activity of a nucleic acid polymerase without using radioisotopes.

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PMA (Propidium monoazide) DNA/RNA binding dye

PMA (Propidium monoazide) DNA/RNA binding dye

Supplier: Biotium

PMA (propidium monoazide) is a high-affinity photoreactive DNA binding dye invented by scientists at Biotium for viability PCR of bacteria and other organisms. The dye is weakly fluorescent by itself but becomes more fluorescent after binding to nucleic acids.

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Lipofuscin autofluorescentie quencher, TrueBlack™

Supplier: Biotium

TrueBlack™ is een reagens voor het doven van lipofuscine-autofluorescentie in weefselcoupes voor immunofluorescentiekleuring. Lipofuscine bestaat uit sterk autofluorescerende korrels van geoxideerde eiwitten en lipiden die zich ophopen in de lysosomen van verouderende cellen. Lipofuscinekorrels fluoresceren helder in alle kanalen die worden gebruikt voor fluorescentiemicroscopie en stapelen zich op in cel- en weefseltypes naarmate ze ouder worden.

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PMA (Propidium monoazide) 20 mM in water DNA/RNA binding dye

PMA (Propidium monoazide) 20 mM in water DNA/RNA binding dye

Supplier: Biotium

PMA (propidium monoazide) dye is a DNA modifier invented by scientists at Biotium. It is a photo-reactive dye that binds to dsDNA with high affinity. Upon photolysis with visible light, PMA dye covalently attaches itself to dsDNA.

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dsDNA quantitation kits for the Qubit® fluorometer, AccuGreen™, high-sensitivity and broad-range

dsDNA quantitation kits for the Qubit® fluorometer, AccuGreen™, high-sensitivity and broad-range

Supplier: Biotium

AccuGreen™ kit is specific for dsDNA and can quantify DNA samples in the range of 0,1 to 10 ng/μl.

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qPCR master mixes, Forget-Me-Not™ EvaGreen®

qPCR master mixes, Forget-Me-Not™ EvaGreen®

Supplier: Biotium

EvaGreen® Dye and high-performance dye-based qPCR master mixes containing EvaGreen® qPCR dye, Cheetah™ HotStart Taq Polymerase, and Forget-Me-Not™ tracking dye. Available with 2-colour tracking or pre-mixed with low or high ROX.

    
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Cell scrapers

Cell scrapers

Supplier: Biotium

PE, disposable, sterile. Mini cell scrapers for harvesting cells or cell lysates from microwell plates.

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Cell viability assay kits

Supplier: Biotium

MTT, XTT, and resazurin are reduced by mitochondrial metabolic activity to yield coloured or fluorescent products, and thus are useful for and assaying cell viability and quantitating cell number. MTT and XTT are reduced to coloured formazin salts that can be measured by absorbance. MTT generates an insoluble formazin salt, requiring cell lysis before the absorbance can be measured, while XTT does not require cell lysis for measurement. Resazurin is a non-fluorescent blue dye that is reduced to the pink fluorescent compound resorufin, which can be measured by fluorescence or absorbance.

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Paraformaldehyde 4% in PBS ready-to-use, fixative reagent

Supplier: Biotium

Paraformaldehyde, 4% in PBS is a ready-to-use fixation solution for cells or tissues.

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Furaptra (Mag-Fura-2) AM Ester ≥95% (by TLC) fluorescent indicator, for calcium determination

Supplier: Biotium

Furaptra, AM ester is a membrane-permeant form of Furaptra (Mag-Fura-2). The indicator is loaded into cells via simple incubation

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Fura 2-AM ≥98% (by HPLC) fluorescent indicator, for calcium determination

Supplier: Biotium

Fura-2, AM ester is a ratiometric fluorescent dye that binds to free intracellular calcium, and is the preferred dye for ratiometric imaging microscopy. Fura-2, AM ester is membrane-permeant and thus can be loaded into cells via incubation.

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Pluronic® F-127

Supplier: Biotium

Pluronic® F-127 is a nonionic detergent useful for solubilizing hydrophobic dye molecules such as cell-permeable AM esters of Fura-2, Fluo-3, Indo-1, or Rhod-2 calcium indicators to facilitate cell loading. Pluronic® F-127 is usually dissolved in water or DMSO with gentle heating.

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Reactive biotin compounds

Supplier: Biotium

Biotium offers reactive biotin compounds with a variety of reactive functional groups.

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5-Aminoallyl-UTP sodium salt, lyophilised powder

Supplier: Biotium

5-Aminoallyl-UTP can be enzymatically incorporated into RNA for subsequent labeling with an amine-reactive fluorescent dye, biotin, or other hapten.

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6-Carboxyrhodamine 6G succinimidyl ester (6-CR6G SE) ≥95% (by TLC) amine-reactive fluorescent dye

Supplier: Biotium

Amine-reactive form of 6-carboxyrhodamine 6G single isomer.

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