PCR cloning kit, CloneJET

CloneJET PCR Cloning Kit, 20 reactions, 1 KIT

Catalogus # K1231

Leverancier: Thermo Fisher Scientific

PCR cloning kit, CloneJET

Catalogus # K1231

Leverancier: Thermo Fisher Scientific

CAS-nummer:

PCR cloning kit, CloneJET

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197,00 €

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Product Details & Documents

The CloneJET PCR cloning kit contains a ready-to-use positive selection cloning vector pJET1.2/blunt. The vector contains a lethal restriction enzyme gene that is disrupted by ligation of a DNA insert into the cloning site. As a result, only bacterial cells with recombinant plasmids are able to form colonies. Recircularised pJET1.2/blunt vector molecules lacking an insert express a lethal restriction enzyme, which kills the host E. coli cell after transformation. This positive selection drastically accelerates the process of colony screening and eliminates additional costs required for blue/white selection.

Specificaties

Specificaties

VPE:
1 KIT
Omschrijving:
CloneJET PCR Cloning Kit, 20 reactions

Specificaties

Acetonitril, anhydrous (max. 0.003% H₂O) ≥99.95%, HiPerSolv CHROMANORM® Reag. USP, Reag. Ph. Eur., ACS, super gradient grade suitable for UPLC/UHPLC instruments

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Methanol ≥99.8%, HiPerSolv CHROMANORM® Reag. Ph. Eur., gradient grade for HPLC

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VWR®, Transferpipetten, Hoge Prestaties

#: 612-1681

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Aceton ≥99%, TECHNICAL

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VWR®, Nitrilhandschoenen

#: 112-2371

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VWR®, Serologische pipetten, standaardreeks

#: 612-3700

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VWR® NITRILE LIGHT, Nitrilhandschoenen

#: 112-2756

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VWR®, gezichtstissues

#: 115-0600

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Acetonitril ≥99.9%, HiPerSolv CHROMANORM® for LC-MS, suitable for UPLC/UHPLC instruments

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Nitril handschoenen, Kimtech™ Purple Nitrile™

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#: 1.00030.2500

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pH-teststrips, MQuant®, Supelco®

#: 1.09535.0001

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Sealing film for manual application, Parafilm® M

#: 291-0057

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Water, HiPerSolv CHROMANORM® for HPLC

#: 23595.328

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Natriumhydroxide 0.1 mol/l (0.1 N) in aqueous solution, AVS TITRINORM Reag. Ph. Eur. volumetric solution

#: 31770.294

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VWR® AVS TITRINORM, Bufferoplossingen, 20 °C

#: 32096.291

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