Recombinant DegP from E. coli

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About this item

DegP acts as a chaperone at low temperatures but switches to a peptidase (heat shock protein) at higher temperatures. It degrades transiently denatured and unfolded proteins which accumulate in the periplasm following heat shock or other stress conditions.

Recombinant protein corresponding to aa 27 - 474 from E. coli degP, fused to His-Tag at N-terminal, expressed in Yeast

DegP is efficient with Val-Xaa and Ile-Xaa peptide bonds, suggesting a preference for beta-branched side chain amino acids. Only unfolded proteins devoid of disulfide bonds appear capable of being cleaved, thereby preventing non-specific proteolysis of folded proteins. Its proteolytic activity is essential for the survival of cells at elevated temperatures. It can degrade IciA, ada, casein, globin and PapA. DegP shares specificity with DegQ. DegP is also involved in the biogenesis of partially folded outer-membrane proteins (OMP).

Specifications

Conjugatie:
Onconjugeerd
Protein/peptide type:
Recombinant
Bron:
E. coli
Storage conditions:
May be stored at 4 °C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at –20 °C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Gen-ID:
947139
Aminozuurgetal:
27 - 474
Protein synonyms:
htrA|Protease Do|Periplasmic serine endoprotease DegP|Heat shock protein DegP|b0161
UniProtKB:
P0C0V0
Protein/peptide name:
DegP
Zuiverheid:
≥90% (SDS-PAGE)
Moleculair gewicht:
48,8 kD
Sequentie:
AETSSATTAQQMPSLAPMLEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGG
NGGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRSDIALIQIQNPKNLTAIK
MADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNAENYENFIQTDAAINRGNSGGALVNLNGELIGINTAIL
APDGGNIGIGFAIPSNMVKNLTSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIK
AGDVITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSSIFNGIEGAEMSNKGK
DQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKVLDSKPSVLALNIQRGDSTIYLLMQ
Formulering:
Supplied as a liquid in 20 mM Tris-HCl, pH 8,0, 50% glycerol
Shipping temperature:
4 °C