Chromogen/Substrate Set

The HIGHDEF® DAB Chromogen/Substrate Set can be used in conjunction with peroxidase-based immunostaining systems. Peroxidase from the antibody detection system reacts with hydrogen peroxide substrate to degrade it, which then reacts with DAB, precipitating it at positive sites and yielding a dark brown color.

• Strong, clear color development
• Compatible with high-throughput systems

3,3'-Diaminobenzidine (DAB), a widely used chromogen for immunoperoxidase staining, is well accepted among pathologists because of its increased sensitivity and decreased background when compared to amino ethyl carbazole (AEC). Specimens stained with DAB can be dehydrated, cleared, and mounted for permanent record keeping. HIGHDEF® DAB Chromogen/Substrate Set is more sensitive and stable than traditional working DAB solutions.