This experiment introduces students to Southern blotting as a tool for DNA fingerprinting in a hypothetical paternity determination. DNA fragments are first separated by agarose gel electrophoresis, then transferred to a nylon membrane and finally visualised by staining.

• Voor 5 labgroepen
• Elektroforese 45 minuten, blotting 's nachts, kleuring en ontkleuring 10 minuten

Also required: Electrophoresis tank and power supply, 65 °C water batch, DNA visualisatoin system, staining net and tray, automatic micropipettes, lab glassware, microwave oven, distilled water, NaCl, NaOH, concentrated HCl, plastic wrap, and forceps.