Yeast viability staining kits

The Yeast Viability Staining Kit is a fluorescent assay to determine yeast viability. Each kit contains two fluorescent components: CF® Dye-labeled Concanavalin A (Con A) and Live-or-Dye™ viability dye. Biotium offers 3 different color combinations to choose from.

• Stable and selective dead cell staining
• Simple, rapid, no-wash staining protocol
• Tolerates fixation and permeabilization
• Suitable for flow cytometry and microscopy
• Three color combinations to choose from

When both dyes are used to stain yeast, live cells display bright fluorescent cell wall staining (Con A), while dead cells with compromised membrane activity exhibit both cell wall staining and cytoplasmic staining (Live-or-Dye™). The dye labeling is extremely stable, allowing the cells to be fixed and permeabilized without loss of fluorescence or dye transfer between cells.

Live-or-Dye™ viability dyes are cell membrane impermeable amine-reactive dyes designed for discrimination between live and dead cells during flow cytometry or microscopy. They are able to enter into dead cells that have compromised membrane integrity and covalently label free amines on intracellular proteins.

Concanavalin A (Con A) is a widely used lectin that selectively binds to α-mannopyranosyl and α-glucopyranosy residues, found in the cell wall of yeast and fungi. It is labeled with one of our superior bright and photostable CF® Dyes.

Green/Red: CF®488A Con A and Live-or-Dye™ 594/614
Red/Blue: CF®594 Con A and Live-or-Dye™ 405/452
Far-Red/Red: CF®640R Con A and Live-or-Dye™ 568/583