BL21 competent cells
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BL21 competent cells are an all-purpose strain for high-level protein expression and easy induction.
- High-level protein expression and easy induction
- Can be used with non-T7 RNA polymerase protein expression systems
- Allows the tightest control of protein expression for extremely toxic proteins
- Suitable for expressing proteins from all species, including mammals
- Ideal for difficult protein expression, especially when codon bias is a problem
- Increases transformation efficiency with Hte phenotype
- Derived from Agilent’s high performance BL21-Gold competent cell line
BL21 competent cells are an all-purpose strain for high-level protein expression and easy induction. The basic BL21 strain does not contain the T7 RNA polymerase gene and can be used with non-T7 RNA polymerase protein expression systems. BL21 has the tightest control of protein expression for extremely toxic proteins.
Agilent BL21-CodonPlus competent cells dramatically improve protein expression in E. coli by overcoming codon bias. Codon bias occurs when forced high-level expression of a gene containing codons rarely expressed in E. coli depletes internal tRNA pools. This often results in poor protein synthesis, early termination of the polypeptide chain, or misincorporation of amino acids into the expressed protein. BL21-CodonPlus competent cells are derived from Agilent’s high performance BL21-Gold competent cell line. These cells enable efficient high-level expression of heterologous proteins in Escherichia coli.;Overcoming codon bias saves time and labour by eliminating the need for site-directed mutagenesis or for expressing the protein in a eukaryotic expression system. L21-CodonPlus competent cells are derivatives of BL21-Gold cells that have been engineered to include extra copies of genes that encode tRNAs for these rare E. coli codons. The additional availability of these tRNAs facilitates high level expression of many heterologous recombinant genes in BL21-CodonPlus competent cells. Efficient production of heterologous proteins in E. coli is frequently limited by the rarity of certain tRNAs that are abundant in the organisms from which the heterologous proteins are derived. Forced high-level expression of heterologous proteins can deplete the pool of rare tRNAs and stall translation.
BL21-CodonPlus competent cells also feature the Hte phenotype present in Agilent’s highest efficiency competent cell strain, XL10-Gold. The presence of the Hte phenotype increases the transformation efficiency of the cells. In addition, the gene that encodes endonuclease I (endA), an enzyme that rapidly degrades plasmid DNA isolated by most miniprep procedures, has been inactivated in these cells. These two features enable direct cloning of many protein expression constructs.
BL21-gold competent cells feature the high transformation efficiency phenotype (Hte) and have the gene encoding endonuclease I (endA) inactivated. Together, these two features allow direct cloning for most protein expression constructs. By cloning directly in the strain, you can save two days of work normally spent on subcloning procedures. The DE3-derivatives of BL21 contain the T7 RNA polymerase gene controlled by the lacUV5 promoter. This all-purpose derivative yields high-level expression and provides easy induction. Use this derivative with nontoxic proteins.
Caution: For research use only. Not for use in diagnostic procedures.
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BL21 competent cells are an all-purpose strain for high-level protein expression and easy induction.
- High-level protein expression and easy induction
- Can be used with non-T7 RNA polymerase protein expression systems
- Allows the tightest control of protein expression for extremely toxic proteins
- Suitable for expressing proteins from all species, including mammals
- Ideal for difficult protein expression, especially when codon bias is a problem
- Increases transformation efficiency with Hte phenotype
- Derived from Agilent’s high performance BL21-Gold competent cell line
BL21 competent cells are an all-purpose strain for high-level protein expression and easy induction. The basic BL21 strain does not contain the T7 RNA polymerase gene and can be used with non-T7 RNA polymerase protein expression systems. BL21 has the tightest control of protein expression for extremely toxic proteins.
Agilent BL21-CodonPlus competent cells dramatically improve protein expression in E. coli by overcoming codon bias. Codon bias occurs when forced high-level expression of a gene containing codons rarely expressed in E. coli depletes internal tRNA pools. This often results in poor protein synthesis, early termination of the polypeptide chain, or misincorporation of amino acids into the expressed protein. BL21-CodonPlus competent cells are derived from Agilent’s high performance BL21-Gold competent cell line. These cells enable efficient high-level expression of heterologous proteins in Escherichia coli.;Overcoming codon bias saves time and labour by eliminating the need for site-directed mutagenesis or for expressing the protein in a eukaryotic expression system. L21-CodonPlus competent cells are derivatives of BL21-Gold cells that have been engineered to include extra copies of genes that encode tRNAs for these rare E. coli codons. The additional availability of these tRNAs facilitates high level expression of many heterologous recombinant genes in BL21-CodonPlus competent cells. Efficient production of heterologous proteins in E. coli is frequently limited by the rarity of certain tRNAs that are abundant in the organisms from which the heterologous proteins are derived. Forced high-level expression of heterologous proteins can deplete the pool of rare tRNAs and stall translation.
BL21-CodonPlus competent cells also feature the Hte phenotype present in Agilent’s highest efficiency competent cell strain, XL10-Gold. The presence of the Hte phenotype increases the transformation efficiency of the cells. In addition, the gene that encodes endonuclease I (endA), an enzyme that rapidly degrades plasmid DNA isolated by most miniprep procedures, has been inactivated in these cells. These two features enable direct cloning of many protein expression constructs.
BL21-gold competent cells feature the high transformation efficiency phenotype (Hte) and have the gene encoding endonuclease I (endA) inactivated. Together, these two features allow direct cloning for most protein expression constructs. By cloning directly in the strain, you can save two days of work normally spent on subcloning procedures. The DE3-derivatives of BL21 contain the T7 RNA polymerase gene controlled by the lacUV5 promoter. This all-purpose derivative yields high-level expression and provides easy induction. Use this derivative with nontoxic proteins.
Caution: For research use only. Not for use in diagnostic procedures.
Product Family Options
Productinformatie
- EigenschapInfo VPEBeschikbaarheidPrice
- AGLS200133BL21 competent cellsBL21 competent cellsFor extremely toxic proteins, use with lambda CE6 under the control of T7 RNA polymerase or use with non-T7 polymerase-based systems5×0,2 mlLog in voor beschikbaarheid1 * 1 mL180,00 €
- AGLS200131BL21(DE3) competent cellsBL21(DE3) competent cellsFor non-toxic proteins, encodes T7 RNA polymerase under the control of the lacUV5 promoter. Use with Variflex or Affinity pCAL vectors.5×0,2 mlLog in voor beschikbaarheid1 * 1 mL180,00 €
- AGLS200132BL21(DE3) pLysS competent cellsBL21(DE3) pLysS competent cellsFor both toxic and non-toxic proteins, pLysS codes for T7 lysozyme, a T7 RNA polymerase inhibitor. Use with pET or pCAL vectors.5×0,2 mlLog in voor beschikbaarheid1 * 1 mL180,00 €
- AGLS230130BL21-Gold competent cellsBL21-Gold competent cellsBL21-Gold competent cells10×0,1 mlLog in voor beschikbaarheid1 * 1 mL223,00 €
- AGLS230245BL21-codonplus (DE3)-RIL competent cellsBL21-codonplus (DE3)-RIL competent cellsEncodes T7 RNA polymerase under control of the lacUV5 promoter for easy protein expression, Use with pET or pCAL vectors, 1×10⁷ cfu/µg of supercoiled DNA10×0,1 mlLog in voor beschikbaarheid1 * 1 mL278,00 €
- STRA200132BL21(DE3) pLysS competent cellsBL21(DE3) pLysS competent cellsFor both toxic and non-toxic proteins, pLysS codes for T7 lysozyme, a T7 RNA polymerase inhibitor. Use with pET or pCAL vectors.5×0,2 mlDiscontinued-
- AGLS230132BL21-Gold (DE3) competent cellsBL21-Gold (DE3) competent cellsBL21-Gold (DE3) competent cells10×0,1 mlLog in voor beschikbaarheid1 * 1 mL223,00 €
- AGLS230280BL21-codonplus (DE3)-RIPL competent cellsBL21-codonplus (DE3)-RIPL competent cellsThese cells contain extra copies of rare E. coli argU, ileY, leuW, proL tRNA genes which corrects for codon bias and dramatically improves expression of sequences from other organisms.10×0,1 mlLog in voor beschikbaarheid1 * 1 mL278,00 €
- AGLS230255BL21-codonplus (DE3)-RP competent cellsBL21-codonplus (DE3)-RP competent cellsEncodes T7 RNA polymerase under control of the lacUV5 promoter for easy protein expression, Use with pET or pCAL vectors, 1×10⁷ cfu/µg of supercoiled DNA.10×0,1 mlLog in voor beschikbaarheid1 * 1 mL278,00 €
- STRA230245BL21-codonplus (DE3)-RIL competent cellsBL21-codonplus (DE3)-RIL competent cellsEncodes T7 RNA polymerase under control of the lacUV5 promoter for easy protein expression, Use with pET or pCAL vectors, 1×10⁷ cfu/µg of supercoiled DNA10×0,1 mlLog in voor beschikbaarheid1 * 1 STSpecificaties:MarketsourceMarketsource
- AGLS230240BL21-codonplus -RIL competent cellsBL21-codonplus -RIL competent cellsFor extremely toxic proteins, use with lambda CE6 under the control of T7 RNA polymerase, 1×10⁷ cfu/µg of supercoiled DNA.10×0,1 mlLog in voor beschikbaarheid1 * 1 mL278,00 €
- AGLS230134BL21-gold (DE3)pLysS competent cellsBL21-gold (DE3)pLysS competent cellsFor both toxic and non-toxic proteins, pLysS codes for T7 lysozome, a T7 RNA polymerase inhibitor. Use with Variflex or Affinity pCAL vectors. Efficiency is ≥1×10⁸ cfu/ µg of supercoiled DNA.10×0,1 mlLog in voor beschikbaarheid1 * 1 mL223,00 €
- STRA230130BL21-gold competent cellsBL21-gold competent cellsFor extremely toxic proteins, use with lambda CE6 under the control of T7 RNA polymerase or use with non-T7 polymerase-based system. Efficiency is ≥1×10⁸ cfu/µg of supercoiled DNA.10×0,1 mlDiscontinued-
- STRA230132BL21-gold (DE3) competent cellsBL21-gold (DE3) competent cellsFor non-toxic proteins, encodes T7 RNA polymerase under the control of the lacUV5 promoter. Use with Variflex or Affinity pCAL vectors. Efficiency is ≥1×10⁸ cfu/µg of supercoiled DNA.10×0,1 mlDiscontinued-
- STRA230134BL21-gold (DE3)pLysS competent cellsBL21-gold (DE3)pLysS competent cellsFor both toxic and non-toxic proteins, pLysS codes for T7 lysozome, a T7 RNA polymerase inhibitor. Use with Variflex or Affinity pCAL vectors. Efficiency is ≥1×10⁸ cfu/ µg of supercoiled DNA.10×0,1 mlDiscontinued-
- STRA230240BL21-codonplus -RIL competent cellsBL21-codonplus -RIL competent cellsFor extremely toxic proteins, use with lambda CE6 under the control of T7 RNA polymerase, 1×10⁷ cfu/µg of supercoiled DNA.10×0,1 mlDiscontinued-
- STRA230250BL21-codonplus -RP competent cellsBL21-codonplus -RP competent cellsFor extremely toxic proteins, use with lambda CE6 under the control of T7 RNA polymerase, 1×10⁷ cfu/µg of supercoiled DNA.10×0,1 mlDiscontinued-
- STRA230255BL21-codonplus (DE3)-RP competent cellsBL21-codonplus (DE3)-RP competent cellsEncodes T7 RNA polymerase under control of the lacUV5 promoter for easy protein expression, Use with pET or pCAL vectors, 1×10⁷ cfu/µg of supercoiled DNA.10×0,1 mlDiscontinued-
- STRA230265BL21-codonplus (DE3)-RIL-X competent cellsBL21-codonplus (DE3)-RIL-X competent cellsEncodes T7 RNA polymerase under control of the lacUV5 promoter for easy protein expression plus methionine label, Use with pET or pCAL vectors, Used for labeling cells 1×10⁷ cfu/µg of supercoiled DNA.10×0,1 mlDiscontinued-
- STRA230275BL21-codonplus (DE3)-RP-X competent cellsBL21-codonplus (DE3)-RP-X competent cellsEncodes T7 RNA polymerase under control of the lacUV5 promoter for easy protein expression plus methionine label, Use with pET or pCAL vectors, Used for labeling cells 1×10⁷ cfu/µg of supercoiled DNA.10×0,1 mlDiscontinued-
- STRA230280BL21-codonplus (DE3)-RIPL competent cellsBL21-codonplus (DE3)-RIPL competent cellsThese cells contain extra copies of rare E. coli argU, ileY, leuW, proL tRNA genes which corrects for codon bias and dramatically improves expression of sequences from other organisms.10×0,1 mlDiscontinued-
- AGLS235200Lambda CE6 Induction kit, Expression of toxic proteins in BL21 and BL21-Gold cellsLambda CE6 Induction kit, Expression of toxic proteins in BL21 and BL21-Gold cellsThese cell contains Bacteriophage CE6, BL21 cells and LE392 cells1 kitLog in voor beschikbaarheid1 * 1 kt558,00 €
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