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Nuclear extract from HeLa cells

Nuclear extractor, 100 µl
  ENZOBMLKI1400100
 :   Enzo Life Sciences
Nuclear extract from HeLa cells
  ENZOBMLKI1400100
 :  Enzo Life Sciences
 :  
Nuclear extract from HeLa cells

Nuclear extractor, 100 µl

 
 
 
 :  
 
170,00 €
   
1 * 100 µl
 

Prepared by high salt extraction of HeLa nuclei (human cervical cancer cell line), this extract is a rich source of HDAC activity, useful for inhibitor screening or immunoprecipitation of HDAC containing complexes. Typically, 0,5 µl or less per well is sufficient for assay with the HDAC FLUOR DE LYS® fluorometric activity assay.

 

 

  • VPE:
    100 µl
  • Omschrijving:
    Nuclear extractor
  • Cat. No.:
    ENZOBMLKI1400100
  • Opslagbuffer:
    Liquid. In 0,1 M KCl, 20 mM HEPES/NaOH, pH 7,9, 20% (v/v) glycerol, 0,2 mM EDTA, 0,5 mM DTT, 0,5 mM PMSF.

 

   
Acetonitril, anhydrous (max. 0.003% H₂O) ≥99.95%, HiPerSolv CHROMANORM® Reag. USP, Reag. Ph. Eur., ACS, super gradient grade suitable for UPLC/UHPLC instruments

  #: 83639.320

 : VWR Chemicals

Methanol ≥99.8%, HiPerSolv CHROMANORM® Reag. Ph. Eur., gradient grade for HPLC

  #: 20864.320

 : VWR Chemicals

VWR®, Transferpipetten, Hoge Prestaties

  #: 612-1681

 : VWR Collection

VWR® NITRILE LIGHT, Nitrilhandschoenen

  #: 112-2755

 : VWR Collection

Aceton ≥99%, TECHNICAL

  #: 20063.365

 : VWR Chemicals

VWR®, Nitrilhandschoenen

  #: 112-2371

 : VWR Collection

VWR®, Serologische pipetten, standaardreeks

  #: 612-3700

 : VWR Collection

VWR®, gezichtstissues

  #: 115-0600

 : VWR Collection

Acetonitril ≥99.9%, HiPerSolv CHROMANORM® for LC-MS, suitable for UPLC/UHPLC instruments

  #: 83640.320

 : VWR Chemicals

Nitril handschoenen, Kimtech™ Purple Nitrile™

  #: 112-4847

 : Ansell

Acetonitril ≥99.9%, LiChrosolv® Reag. Ph. Eur., gradient grade for liquid chromatography, Supelco®

  #: 1.00030.2500

 : Merck

pH-teststrips, MQuant®, Supelco®

  #: 1.09535.0001

 : Merck

Sealing film for manual application, Parafilm® M

  #: 291-0057

 : HEATHROW

Water, HiPerSolv CHROMANORM® for HPLC

  #: 23595.328

 : VWR Chemicals

Natriumhydroxide 0.1 mol/l (0.1 N) in aqueous solution, AVS TITRINORM Reag. Ph. Eur. volumetric solution

  #: 31770.294

 : VWR Chemicals

VWR® AVS TITRINORM, Bufferoplossingen, 20 °C

  #: 32096.291

 : VWR Chemicals

Water, HiPerSolv CHROMANORM® for LC-MS, suitable for UPLC/UHPLC instruments

  #: 83645.320

 : VWR Chemicals

VWR® Nitrile Extra Light, Nitrilhandschoenen

  #: 112-4195

 : VWR Collection

Bufferoplossingen, gebruiksklaar, waarnaar wordt verwezen bij 20 °C, CertiPUR®, Supelco®

  #: 1.09439.1000

 : Merck

epT.I.P.S.® Bulk - Pipette Tips

  #: 613-3508

 : EPPENDORF