Cell surface protein isolation kit, Pierce™

The Pierce™ Cell Surface Protein Isolation Kit is a complete set of reagents for selective biotinylation and subsequent purification of mammalian cell surface proteins to the exclusion of intracellular proteins.

• Isolates cell surface proteins thereby reducing complexity of total cellular protein
• Cleavable biotin allows for nearly 100% recovery of isolated cell surface proteins
• Proteins recovered in SDS-PAGE buffer are loaded directly onto polyacrylamide gels
• Protocol designed for diverse cell lines, including NIH 3T3, HeLa, C6 and A431

The kit efficiently labels proteins that have accessible lysine residues and sufficient extracellular exposure. The isolation procedure uses a cell-impermeable, cleavable biotinylation reagent (Sulfo-NHS-SS-Biotin) to label exposed primary amines of proteins on the surface of intact adherent or suspension cells. Treated cells are then harvested, lysed and the labeled surface proteins are affinity-purified.