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Kleuringen voor elektroforese

Kleuringen voor elektroforese

Klaar-voor-gebruik elektroforesebuffers, -gels en poederkleurstoffen sorteren snel monstermoleculen op basis van grootte en lading. Deze reagentia produceren minder achtergrondfluorescentie die visuele studies negatief kan beïnvloeden. Om de vereiste chemische reactie te bereiken, selecteert u het type kleurstof op basis van de omgevingsomstandigheden en de doelstellingen van het experiment. Van het veelgebruikte Coomassie Brilliant Blue tot de kankersonderzoeks-specifieke Papanicolaou maken gevoelige elektroforesekleuringen celcomponenten zichtbaar voor verdere DNA- en RNA-analyses.

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Peqlab peqGREEN, DNA/RNA Dye

Peqlab peqGREEN, DNA/RNA Dye

Supplier: VWR Collection

peqGREEN dye is a safe alternative to ethidium bromide for staining of dsDNA, ssDNA and RNA.

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VWR® BANDit™ Protein Stain Blue

VWR® BANDit™ Protein Stain Blue

Supplier: VWR Chemicals

Blue BANDit is a safe and environmentally friendly alternative to traditional Coomassie® Blue staining.

   Duurzame opties beschikbaar
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VWR®, Loading dye base

VWR®, Loading dye base

Supplier: VWR Chemicals

This loading dye base is used for custom loading buffer preparation. It is a concentrated solution containing patent blue, bromophenol blue and amaranth tracking dyes, which migrate at approximately 4 000, 400 and 10 bp, on a 1% agarose gel, respectively.

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VWR®, Nucleic acid loading dyes

VWR®, Nucleic acid loading dyes

Supplier: VWR Chemicals

Designed to optimise loading nucleic acids in agarose gels, the loading dye imparts colour to the sample to facilitate the loading process and increase the density of the sample to ensure efficient sample distribution into each well. The dye migrates independently from the sample, allowing estimation of the migration of nucleic acids.

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VWR® EZ-Vision®, DNA-kleurstoffen

VWR® EZ-Vision®, DNA-kleurstoffen

Supplier: VWR Chemicals

EZ-Vision DNA-kleurstof als laadbuffer biedt een niet-mutageen, milieuvriendelijk alternatief voor ethidiumbromide voor onmiddellijke visualisatie van fluorescerende DNA-banden.

   Duurzame opties beschikbaar
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Nucleic acid stains, PAGE GelRed™

Nucleic acid stains, PAGE GelRed™

Supplier: Biotium

Stains specifically designed for DNA in polyacrylamide gels.

   Duurzame opties beschikbaar
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Glycoprotein stain

Glycoprotein stain

Supplier: G-Biosciences

The stain uses an enhanced Periodic Acid-Schiff (PAS) method for detection of glycoprotein sugars. The supplied agent first oxidises the cis-diol sugar groups to aldehydes. The aldehyde groups react with the sensitive glycoprotein stain solution forming Schiff bonds and producing strong magenta colour bands. RAPIDstain™ can be used after glycoprotein staining to detect non-glycosylated proteins and the use of the stain enhances glycoprotein staining. The glycoprotein stainis highly convenient as all the key reagents required for staining are supplied and a unique positive & negative control is included.

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EMBER™ Ultra DNA Gel Stain Kit

Supplier: Biotium

The most sensitive DNA gel stain available in convenient pre-coated agarose format with no need for post-electrophoresis staining.

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BLOT-FastStain™

BLOT-FastStain™

Supplier: G-Biosciences

BLOT-FastStain™ is a unique stain for reversible staining of protein on nitrocellulose and PVDF transfer membranes. It only stains protein and leaves the background absolutely untouched and brilliant white resulting in high band visibility.

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Electrophoresis gel stain, Reversible Copper Stain™

Electrophoresis gel stain, Reversible Copper Stain™

Supplier: G-Biosciences

The Reversible Copper Stain™ is a single step stain for the rapid detection of proteins resolved on SDS-PAGE.

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VWR® EZ-Vision® Bluelight DNA Dye 10000X

VWR® EZ-Vision® Bluelight DNA Dye 10000X

Supplier: VWR Chemicals

EZ-Vision BlueLight is a sensitive, non mutagenic and environmentally safe fluorescent dye designed for gel staining.

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VWR®, DNA loading buffers

VWR®, DNA loading buffers

Supplier: VWR Chemicals

These DNA loading dyes are used to load DNA samples to agarose or SDS DNA gels for gel electrophoresis.

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Gelkleurstoffen voor nucleïnezuren, GelRed™ en GelGreen™

Gelkleurstoffen voor nucleïnezuren, GelRed™ en GelGreen™

Supplier: Biotium

GelRed and GelGreen are highly sensitive DNA/RNA gel stains made safe by preventing penetration into cells.

   Duurzame opties beschikbaar
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Gel loading dye, orange (6X)

Gel loading dye, orange (6X)

Supplier: Thermo Fisher Scientific

Oange loading dye (6X) is used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels. Ideal for conventional DNA electrophoresis.

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Silver stain, FOCUS™ FASTsilver™

Silver stain, FOCUS™ FASTsilver™

Supplier: G-Biosciences

FOCUS™ FASTsilver™ produces crystal clear backgrounds and maximal peptide recovery needed for critical analysis by mass spectrometry. Complete proteolytic digestion and recovery of peptides is required for optimal analysis, however silver ions in traditional silver staining kits inhibit proteolytic digestion. In addition, glutaraldehyde, a common sensitiser in silver stains, modifies peptide lysine residues preventing complete digestion and recovery.

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CYGREEN® Nucleïnezuur kleurstof

Supplier: Enzo Life Sciences

Nucleïnezuurkleurstof voor gelkleuring en qPCR met hogere gevoeligheid.

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