Reagentia voor eiwitextractie

Efficiënte cellyse en maximale eiwituitslagen zijn van vitaal belang voor hoogwaardige recombinant-eiwitzuivering. Proteaseremmercocktails zijn bedoeld voor het lyseren van zoogdiercellen, buffers die plasma- en intracellulaire membranen oplossen, zwakke intermoleculaire bindingen verbreken en de meeste eiwitten oplossen. Deze eiwitextractiereagentia, die procedures gemakkelijker maken, kunnen ook worden gebruikt om eiwitten te isoleren uit zowel de cytoplasmatische als de nucleaire fracties van gekweekte cellen.

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Endoproteinase Asp-N, excision grade, Pseudomonas fragi

Supplier: Merck Millipore (Calbiochem‎)

Native endoproteinase Asp-N from Pseudomonas fragi. Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Metalloprotease that specifically hydrolyses proteins at the N-terminal side of aspartic acid and cysteic acid residues. Inhibited by aprotinin, DFP, leupeptin, and TLCK. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.

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Washing solution, Protein-OUT™

Supplier: G-Biosciences

Protein-OUT™ is a washing solution that cleans plastic and glassware used in the proteolytic digestion and peptide isolation for mass spectrometry analysis. The rinsing of plastic and glassware with Protein-OUT™ will remove interfering chemicals and peptides that can leach from the tube walls and interfere or suppress the mass spectrometry signal. In addition, rinsing with Protein-OUT™ removes inadvertent contamination with dust and keratin.

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Uniform latex particles for immobilisation of biomolecules, PL-Latex

Supplier: VARIAN

Uniform latex particles are used in a wide range of applications that exploit their physico-chemical properties in sophisticated immunoassay formats, both in medicine and veterinary science research.

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Surfactant, RapiGest™ SF

Supplier: WATERS

RapiGest™ SF is an anionic surfactant for peptide digestion. Supplied as five 1 mg vials for 1 to 50 digestions. The digestion speed and more peptide recovery is enhanced by using trypsin or other enzymes.