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Reagentia voor eiwitpurificatie

Reagentia voor eiwitpurificatie

Eiwitzuiveringsreagentia worden gebruikt voor het isoleren van één of meer eiwitten uit een complex mengsel, meestal cellen, weefsels of volledige organismen. Dit proces is van vitaal belang voor de karakterisering van functie en structuur, evenals voor het identificeren van interacties van het eiwit van belang. Tijdens de vele stappen helpen eiwitzuiveringsreagentia bij cellyse, het scheiden van de oplosbare eiwitcomponenten van celresten en uiteindelijk het scheiden van het eiwit van belang van product- en procesgerelateerde onzuiverheden.

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Recombinant protein A, Pierce™

Recombinant protein A, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Recombinant Protein A is useful as the basis for preparing various kinds of probes or affinity media for detection or purification of rabbit and human antibodies, especially IgG isotypes, in immunoassays and antibody purification protocols.

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Recombinant protein L, peroxidase conjugated, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Recombinant Peroxidase Conjugated Protein L can be used to probe and detect rabbit and human antibodies, especially IgG isotypes, in Western blotting, ELISA, IHC and other immunoassay protocols.

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Pegylation reagents, Methyl-PEG-NHS-Maleimide, Pierce™

Pegylation reagents, Methyl-PEG-NHS-Maleimide, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ MM(PEG)n reagents are methyl-terminated PEG compounds activated with a maleimide group for covalent pegylation of sulfhydryls on proteins or assay surfaces.

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Immobilised GST agarose, Pierce™

Immobilised GST agarose, Pierce™

Supplier: Thermo Fisher Scientific

Immobilised GST is glutathione S-transferase from Schistosoma japonicum that has been covalently immobilised on crosslinked 6% beaded agarose.

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Immobilised jacalin agarose, Pierce™

Immobilised jacalin agarose, Pierce™

Supplier: Thermo Fisher Scientific

Jacalin is an alpha-D-galactose binding lectin extracted from jack-fruit seeds (Artocarpus integrifolia). This lectin is a glycoprotein (approx. 40 kDa) composed of four identical subunits. Jacalin binds to human IgA1 (also called serum or secretory IgA) with high specificity (i.e., it does not bind IgG or IgM). Thus, Jacalin that has been immobilised on crosslinked beaded agarose is useful for purifying human IgA from serum or colostrum samples.

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Streptavidin HRP conjugates, high sensitivity, Pierce™

Streptavidin HRP conjugates, high sensitivity, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ High Sensitivity Streptavidin-HRP is a peroxidase-conjugated streptavidin biotin-binding protein that provides signal amplification like poly-HRP and exceptional storage stability.

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Iminobiotin agarose, Pierce™

Iminobiotin agarose, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Iminobiotin Agarose is for the purification of avidin, streptavidin or Thermo Scientific NeutrAvidin® Conjugates. Iminobiotin is a cyclic guanido analogue of biotin and has a lower affinity constant for binding avidin, streptavidin or NeutrAvidin® Protein.

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Recombinant protein A/G, Pierce™

Recombinant protein A/G, Pierce™

Supplier: Thermo Fisher Scientific

Purified (unconjugated) Pierce™ Recombinant Protein A/G is useful as the basis for preparing various kinds of probes or affinity media for detection or purification of rabbit and human antibodies, especially IgG isotypes, in immunoassays and antibody purification protocols.

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Maleimide-activated protein, NeutrAvidin™ Pierce™

Supplier: Thermo Fisher Scientific

Thermo Scientific™ Maleimide Activated NeutrAvidin™ Protein is a specially-prepared form of avidin that decreases background in biotin-binding.

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Pierce™ Pegylation Reagents, Carboxy-PEG-Amine Compounds

Pierce™ Pegylation Reagents, Carboxy-PEG-Amine Compounds

Supplier: Thermo Fisher Scientific

The carboxy-PEGn-amine (CA[PEG]n) PEGylation reagents are zwitterionic, amino acid derivatives that are used for modifying proteins or surfaces such as beads, nanoparticles and self-assembled monolayers. These PEGylation reagents are homogenous compounds of defined molecular weight and spacer length, providing precision in optimising modification applications.

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Pierce™ Protein A

Pierce™ Protein A

Supplier: Thermo Fisher Scientific

Protein A can be used to probe and detect rabbit and human antibodies, especially IgG isotypes, in Western blotting, ELISA, IHC and other immunoassay protocols.

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Recombinant protein L, Pierce™

Recombinant protein L, Pierce™

Supplier: Thermo Fisher Scientific

Purified (unconjugated) Pierce™ Recombinant Protein L is useful as the basis for preparing various kinds of probes or affinity media for detection or purification of mouse and human antibodies in immunoassays and antibody purification protocols.

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ReadiPrep™ Nickel NTA Magnetic Beads

Supplier: AAT Bioquest

ReadiPrep™ Nickel NTA magnetic beads provide a fast and simple solution for small-scale affinity purification of histidine-tagged proteins.

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Immobilised ficin (agarose resin), Pierce™

Immobilised ficin (agarose resin), Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Immobilised Ficin consists of a sulfhydryl-specific protease (ficin) that has been immobilised onto beaded agarose resin to enable controlled antibody fragmentation, especially of mouse IgG1.

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TCEP solution, neutral pH, Bond-Breaker™ Pierce™

TCEP solution, neutral pH, Bond-Breaker™ Pierce™

Supplier: Thermo Fisher Scientific

Bond-Breaker™ TCEP Solution (neutral pH) is a stable, 0,5 M solution of the thiol-free, phosphine-based TCEP compound, useful as a 10X stock for addition to SDS-PAGE sample loading buffers to reduce protein disulphide bonds.

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Recombinant protein A/G, alkaline phosphatase conjugated, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Recombinant Alkaline Phosphatase Conjugated Protein A/G is useful to probe and detect antibodies, especially IgG isotypes, of many different species hosts in Western blotting, ELISA, IHC and other immunoassay protocols.

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