U hebt gezocht naar: Reagentia voor nucleïnezuren
Nucleïnezuurreagentia vormen de basis van genetische analyse en moleculaire biologie en spelen een cruciale rol van klonering tot genexpressieonderzoeken. Verdiep je in onze collectie met enzymen van hoge kwaliteit voor eindpunt-PCR, geavanceerde reagentia voor isotherme amplificatie en uitgebreide kits voor de zuivering van nucleïnezuren. Voor precisie in kwantitatieve assays, bekijk onze selectie van qPCR- en RT-qPCR-kits, geoptimaliseerd voor ongeëvenaarde nauwkeurigheid. Ons portfolio omvat ook next-generation sequencing reagentia, die geavanceerde oplossingen bieden voor genomische sequencing en gepersonaliseerde geneeskunde.
sparQ PureMag beads
Supplier: Quantabio
sparQ PureMag beads is a fast and reliable nucleic acid purification system for reaction cleanup and size selection in Next Generation Sequencing (NGS) workflows. Based on the reversible nucleic acid-binding properties of magnetic beads; this product can be used to quickly remove primers, primer-dimers, unincorporated nucleotides, salts, adapters and adapter-dimers from NGS library prep reactions to improve downstream sequencing performance. sparQ PureMag beads allows excellent recovery of fragments greater than 100 bp without centrifugation or filtration. Consistent and reliable size selection can be achieved by simply adjusting the beads to sample ratio. This product is designed for both manual and automated processing, allowing seamless integration into existing workflows.
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cDNA-PCR Sequencing Kits
Supplier: Oxford Nanopore Technologies
The cDNA-PCR Sequencing Kits enable the identification and quantification of full-length transcripts with highest output using nanopore sequencing.
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PCR barcoding expansion
Supplier: Oxford Nanopore Technologies
PCR Barcoding Expansion packs enable multiple nanopore sequencing libraries to be pooled and run on a single MinION Flow Cell.
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16S Barcoding Kits
Supplier: Oxford Nanopore Technologies
The 16S Barcoding Kit delivers rapid, genus-level bacteria identification using nanopore sequencing.
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Restriction enzymes, AgeI, FastDigest™, Fermentas
Supplier: Thermo Fisher Scientific
Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.
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Restriction enzymes, EcoRII, 10 U/µl, Fermentas
Supplier: Thermo Fisher Scientific
CCWGG sites and cuts best at 37 °C in O buffer.
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Restriction enzymes, Eco32I (EcoRV) (HC), Fermentas
Supplier: Thermo Fisher Scientific
ATC sites and cuts best at 37 °C in R buffer Isoschizomers: EcoRV.
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Restriction enzymes, BamHI, HC, Fermentas
Supplier: Thermo Fisher Scientific
GATCC sites and cuts best at 37 °C in its own unique buffer.
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Blur 8 BioProbe®
Supplier: ENZO LIFE SCIENCES
The Blur 8 BioProbe® labeled probe is prepared by nick translation of a clone of the human alu repeat sequence. Fragment size range: 100-1000 base pairs (as estimated by agarose gel electrophoresis).
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BK virus BioProbe®
Supplier: ENZO LIFE SCIENCES
The BK Virus BioProbe® labeled probe is prepared by nick translation of a clone of the entire BK virus genome. BK virus, a simian polyoma virus, is related to SV40 and also bears some similarity to the human virus JC virus. BK virus is highly tumorigenic in hamsters. Fragment size range: 100-1000 base pairs (as estimated by agarose gel electrophoresis).
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DNA fragment and library prep kit, sparQ
Supplier: Quantabio
The sparQ DNA Frag and Library Prep kit optimises the integration of enzymatic fragmentation into a two-step protocol for the streamlined construction of libraries for sequencing on Illumina® NGS platforms. A single-tube enzyme mix facilitates the combination of fragmentation and DNA polishing minimising DNA over fragmentation while greatly simplifying library.
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Long Fragment Buffer Expansion
Supplier: Oxford Nanopore Technologies
Additional Long Fragment Buffer (LFB).
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Next generation sequencing (NGS), DNA/RNA clean up Mag-Bind® TotalPure
Supplier: OMEGA BIO-TEK
Mag-Bind® TotalPure NGS offers an easy-to-use, reliable solution for the purification of both DNA and RNA for next-generation sequencing workflows with high recovery rates. Mag-Bind® TotalPure NGS is capable of selectively binding fragments depending on the reagent to sample ratio used, giving the user flexibility to perform left, right or double-sided size selection.
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SFB expansion
Supplier: Oxford Nanopore Technologies
Short Fragment Buffer (SFB).
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Rapid PCR Barcoding Kit 24 V14
Supplier: Oxford Nanopore Technologies
The Rapid PCR Barcoding Kit 24 V14 provides a fast and simple method of preparing up to 24 barcoded samples for sequencing 1-5 ng of gDNA using PCR.
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Yeast transformation kit, Alkali-Cation™, MP Biomedicals
Supplier: MP Biomedicals
Alkali-Cation™ Yeast Transformation Kit provides an efficient method for yeast transformation and has been successfully used to transform linear DNA into Pichia pastoris for protein expression studies.
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ddATP [2',3'-Dideoxyadenosine-5'-triphosphate] *10 mM in ddH₂O* (1 µmole)
Supplier: AAT BIOQUEST
Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.
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2-Aminoethoxypropargyl ddTTP (1 µmole)
Supplier: AAT BIOQUEST
Sanger method is one of the most reliable and earliest DNA sequencing methods.
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2-Aminoethoxypropargyl ddATP (1 µmole)
Supplier: AAT BIOQUEST
Sanger method is one of the most reliable and earliest DNA sequencing methods.
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Restriction enzymes, XhoI, FastDigest™, Fermentas
Supplier: Thermo Fisher Scientific
FastDigest™ XhoI is one of an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest™ and FastDigest™ Green reaction buffers.
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Restriction enzymes, Dpn I restriction enzymes
Supplier: AGILENT
Dpn I is an ideal enzyme for cloning and southern blot analysis.
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Restriction enzymes, BpiI, FastDigest™, fermentas
Supplier: Thermo Fisher Scientific
Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.
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Restriction enzymes, SalI, FastDigest™, Fermentas
Supplier: Thermo Fisher Scientific
Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.
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Cytomegalovirus BioProbe®
Supplier: ENZO LIFE SCIENCES
The Cytomegalovirus (CMV) BioProbe® labeled probe is prepared by nick translation of cloned fragments of the CMV Towne strain. The fragments include a total of 30-31 kb of DNA, approximately 20% of the CMV genome. The probe is specific for cytomegalovirus DNA. It does not hybridize to DNAs of other herpesviruses (i.e., herpes simplex virus or Epstein-Barr virus) but it has been found to hybridize to the DNA of CMV clinical isolates. Fragment size range: 100-1000 base pairs (as estimated by agarose gel electrophoresis).
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Bio-16-ddUTP dideoxynucleotide pack for 3’-oligonucleotide labeling
Supplier: ENZO LIFE SCIENCES
The procedure for labeling of DNA probes with a polynucleotide 'tail' containing hapten-labeled nucleotides was developed by Enzo. In such terminal labeling reactions, terminal transferase catalyzes the addition of nucleotides to any 3'-OH terminus in a template independent manner. This rapid and convenient nonradioactive labeling procedure is free of any sequence bias that is normally observed in random priming or nick translation reactions. Terminal labeling is an ideal procedure for the labeling of oligonucleotides.
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Adenovirus BioProbe®
Supplier: ENZO LIFE SCIENCES
The Adenovirus BioProbe® labeled probe is prepared by nick translation of cloned fragments of the adenovirus 5 genome. The fragments constitute a total of 20 kb of DNA, approximately 56% of the genome. The probe is specific for adenovirus and, in addition to hybridization to adenovirus 5 sequences, the probe will cross hybridize with adenovirus types 4, 7, 11, 20, 40 and 41 due to their considerable sequence homology. Fragment size range: 100-1000 base pairs (as estimated by agarose gel electrophoresis).
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2-Aminoethoxypropargyl ddGTP (1 µmole)
Supplier: AAT BIOQUEST
Sanger method is one of the most reliable and earliest DNA sequencing methods.
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MagaDye™ 588 ddTTP
Supplier: AAT BIOQUEST
Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.
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MagaDye™ 561 ddATP
Supplier: AAT BIOQUEST
Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.
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Transformation kit, EZ-Yeast™, MP Biomedicals
Supplier: MP Biomedicals
The EZ-Yeast™ Transformation Kit is designed for high throughput transformation with efficiencies up to 10³ transformants per μg plasmid DNA. It is ideal for cases where a large number of transformations must be performed, but only a few transformants are needed. Perfect for a quick analysis of potentially positive two-hybrid colonies, it does not require fully competent cells prior to transformation.