Reagentia voor nucleïnezuren

Supplier: TriLink BioTechnologies

This nucleotide consists of an unmodified base, a ribose, and a 5′ triphosphate group for synthesis of RNA molecules.

Supplier: Quantabio

AccuStart™ II PCR ToughMix® is a 2X concentrated ready to use reaction cocktail for PCR amplification of DNA templates that overcomes many known inhibitors of PCR often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues. It contains all components, except primers and template.

Supplier: OMEGA BIO-TEK

Plasmide dat wordt geïsoleerd met traditionele zuiveringsprocedures, bevat gewoonlijk hoge concentraties endotoxinen die aanzienlijk van invloed kunnen zijn op verdere transfectie-experimenten. De E.Z.N.A.®-plasmidekits zonder endotoxinen hebben een efficiënte endotoxineverwijderingsstap in de plasmidezuiveringsprocedure om plasmide met een hoge transfectiekwaliteit te produceren.

Supplier: OMEGA BIO-TEK

Isolate DNA from plant or fungal samples using spin columns.

Supplier: OMEGA BIO-TEK

De E.Z.N.A.® Bacterieel DNA-kit biedt snelle en betrouwbare isolatie van hoogwaardig totaal cellulair DNA uit een breed scala aan bacteriesoorten. Deze kit maakt gebruik van geoptimaliseerde lysisomstandigheden en er kunnen maximaal 1×10⁹ bacteriecellen worden verwerkt per kolom. Er zijn geen organische extracties, wat de hoeveelheid kunststofafval en de werktijd beperkt, zodat meerdere monsters parallel kunnen worden verwerkt. Bacteriecellen worden gekweekt tot de logfase en geoogst. De celwand wordt verwijderd door lysozymdestructie en schudden met glasparels, gevolgd door proteaseafbraak. Na lysis worden de bindingsomstandigheden aangepast en wordt het monster aangebracht op een HiBind DNA-spinkolom. Met twee snelle wasstappen worden zoutresten en eiwitverontreinigingen verwijderd. Tot slot wordt DNA geëlueerd in water of elutiebuffer. Gezuiverd DNA kan direct worden gebruikt in vervolgtoepassingen zonder dat verdere zuivering nodig is.

Supplier: OMEGA BIO-TEK

Gelzuivering van DNA is een techniek die vaak wordt gebruikt voor het isoleren van specifieke DNA-fragmenten. De meeste methoden slagen er echter niet in om agarose volledig te verwijderen (wat kan leiden tot problemen bij vervolghandelingen), breken het DNA of hebben een zeer laag rendement.

Supplier: Thermo Fisher Scientific

Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

Supplier: OMEGA BIO-TEK

RNA-Solv® Reagent is a one reagent system for the isolation of total RNA from cells and tissues. The reagent, a single-phase solution consisting of phenol and guanidine isothiocyanate, is a modification of the single-step RNA isolation method developed by Chomczynski and Sacchi. The sample is homogenised and lysed in RNA-Solv® Reagent, which maintains the integrity of the RNA while disrupting and denaturing endogenous RNases and other cellular components. Extraction of the lysate with chloroform further denatures proteins and separates the mixture into an organic and an aqueous phase. RNA remains exclusively in the aqueous phase, and is subsequently recovered by isopropanol.

Supplier: Quantabio

sparQ Universal Library Quant Kit provides rapid and accurate quantification of libraries prepared for sequencing on Illumina® NGS platforms.

Supplier: AGILENT

For libraries constructed from highly methylated DNA, Gigapack III Packaging Extract simplifies the packaging procedure and increases the efficiency and representation of libraries constructed from highly methylated DNA. Each packaging extract is restriction minus to optimize packaging efficiency and library representation.

Supplier: Thermo Fisher Scientific

AATTC sites and cuts best at 37 °C in its own unique buffer. Exhibit 100% activity in the recommended buffer and reaction conditions.

Supplier: AGILENT

Protein directed evolution in just one step.

Supplier: Quantabio

AccuStart™ Genotyping ToughMix® is a 2X concentrated ready to use reaction cocktail for PCR amplification of DNA templates that overcomes inhibitors often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues. It is a versatile and robust PCR reagent that provides highly specific and efficient amplification for genotyping sequence variants including allelic discrimination of single nucleotide polymorphisms (SNPs) using a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes.

Supplier: Merck

The device utilises gel compression to extract DNA from the agarose. Centrifugal force collapses the gel structure, drives the agarose through a small orifice in the gel nebulizer and the resultant gel slurry is sprayed into the sample filter cup.

Supplier: Apollo Scientific

Recombinant Taq DNA polymerase produced in E.coli 20 U/µl.

Supplier: TriLink BioTechnologies

2',3'-Dideoxycytidine-5'-O-(1-Thiotriphosphate) is a sugar modified nucleoside triphosphate where the 2' and 3' hydroxyl groups are absent, resulting in chain termination. This ddCTP analog also contains a 1-Thiotriphosphate which results in a nuclease resistant phosphorothioate linkage. The inability of polymerases to extend from a dideoxy nucleotide causes chain termination and is useful in antiviral research and in a variety of biotechnology applications.