Reagentia voor nucleïnezuren

Supplier: VWR Chemicals

Fast HiFi DNA Polymerase 2 U/µl is een DNA-polymerase met proefleesfunctie dat de volgende eigenschappen vertoont: hoge nauwkeurigheid (>60x Taq DNA-polymerase), het vermogen om problematische DNA-targets te amplificeren, zoals targets met een laag tot hoog GC-gehalte, en het vermogen om lange DNA-targets te amplificeren. Het wordt aanbevolen voor toepassingen die een extreem hoge nauwkeurigheid vereisen, zoals klonen/subkloneren, NGS-toepassingen en mutagenese.

Supplier: VWR Chemicals

Fast HiFi DNA Polymerase 2 U/µl is a proofreading DNA polymerase displaying the following features; high fidelity >60X Taq DNA Polymerase, ability to amplify problematic DNA targets, such as those with low to high GC content and ability to perform amplification on long DNA targets. lt is recommended for applications, which require extremely high fidelity such as cloning / sub cloning, NGS applications and mutagenesis.

Supplier: VWR Chemicals

VWR® ExoCleanUp FAST PCR reagent is a one-step PCR clean-up reagent for optimal sequencing results, consisting of a balanced combination of a heat-labile exonuclease I (HL-ExoI) and a recombinant shrimp alkaline phosphatase (rSAP).

Supplier: VWR Collection

10X PCR Buffer, incomplete

Supplier: VWR Chemicals

Real-Time PCR kit for detection and quantification of Legionella spp. in environmental samples (ISO/TS 12869). Kit contains all necessary reagents for 100 reactions. This product provides rapid and efficient detection and quantification of Legionella spp.

Supplier: TriLink BioTechnologies

CleanCap® Reagent AU is designed for the co-transcriptional capping of self-replicating or self-amplifying (saRNA) to produce an optimal Cap 1.

Supplier: Quantabio

Ultra-Plex™ 1-Step ToughMix® is a ready-to-use, 4X-concentrated master mix for reverse transcription quantitative PCR (RT-qPCR) of RNA templates using hybridisation probe detection chemistries such as TaqMan® 5’-hydrolysis probes on Real-Time PCR systems that utilise ROX passive reference with 490 nM excitation. First-strand cDNA synthesis and PCR amplification are carried out in the same tube without opening between procedures. It is ideal for highly sensitive quantification of RNA viruses or low abundance RNA targets in uni- or multiplexed RT-qPCR applications as well as high throughput gene-expression studies. The system has been optimised to deliver maximum RT-qPCR efficiency, sensitivity, and specificity in reduced reaction volumes and fast cycle times. Ultra-Plex™ 1-Step ToughMix® contains all required components for RT-qPCR except RNA template and probe. It is compatible with all dual-labeled probe chemistries.

Supplier: Quantabio

PerfeCTa® MultiPlex qPCR ToughMix® is een 5x geconcentreerde, gebruiksklare reactiemengsel voor real-time kwantitatieve PCR (qPCR) die veel bekende inhibitoren van PCR oplost die vaak aanwezig zijn in ruwe monsters die uit milieumonsters, plantenweefsels of dierlijke weefsels zijn geëxtraheerd. Het product bevat alle componenten, behalve primers, probes en templates. De 5x geconcentreerde ToughMix® maakt ook de toevoeging van grotere hoeveelheden sjabloon en verbeterde detectiegevoeligheid bij monsters met lage concentraties mogelijk. PerfeCTa® MultiPlex qPCR ToughMix® is geoptimaliseerd voor maximale PCR-efficiëntie, gevoeligheid en specificiteit in gereduceerde reactievolumes met snelle cycli of conventionele PCR-cyclusprotocollen.

Supplier: OMEGA BIO-TEK

E.Z.N.A.® Plasmid Midi Kit offers a novel and reliable method of isolating up to 200 µg of plasmid DNA in less than 60 minutes. This kit combines HiBind® membrane technology with the time-tested consistency of alkaline SDS lysis of bacterial cells to deliver high-quality plasmid DNA. The DNase/RNase-free HiBind® DNA midi columns operate by eliminating time consuming phenol-chloroform extractions and alcohol precipitations. Plasmid purification using the E.Z.N.A.® Plasmid Midi Kit follows a simple bind-wash-elute procedure thereby making it possible for multiple samples to be processed. Although yields vary according to plasmid copy number, E.coli strain, and conditions of growth, 50 ml of an overnight culture in LB medium will typically produce 200 µg of high-copy number plasmid DNA. When working with low-copy number plasmids the starting culture volume can be increased up to 100 ml. High-quality DNA is ready for immediate use in routine molecular biology applications, such as automated fluorescent sequencing, restriction enzyme digestion, and transfection screening.

Supplier: Thermo Fisher Scientific

Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

Supplier: Enzo Life Sciences

Optimised standard PCR mix for enhanced speed, yield, and specificity. Used for standard PCR applications.

Supplier: TriLink BioTechnologies

Cyanine 5-Aminoallyluridine-5'-Triphosphate is a substrate for T7 RNA polymerase, allowing the incorporation of cyanine 5 into RNA and mRNA during in vitro transcription. Cyanine 5 incorporation allows for fluorescent detection of RNA and mRNA independent of protein translation.

Supplier: TriLink BioTechnologies

2'-Fluoro NTPs are being utilised in an increasing number of applications in research and new drug development. 2'-Fluoro-dCTP is incorporated in both DNA and RNA constructs to improve in vivo stability.

Supplier: OMEGA BIO-TEK

The Mag-Bind® cfDNA Kit CE IVD is designed for rapid and reliable isolation of circulating cell-free DNA from 1 to 4 ml plasma/serum samples.

Supplier: OMEGA BIO-TEK

Het E-Z Select® Robotic Reservoir van 600 ml is ontworpen voor het bewaren van grote hoeveelheden reagens voor gebruik op geautomatiseerde platforms zoals de Hamilton Microlab STAR.

Supplier: Oxford Nanopore Technologies

Additional rapid adapter and adapter buffer to enable customers to maximise the use of rapid barcoding kits 24 and 96 V14 (SQK-RBK114.24 and SQK-RBK114.96).