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The electrophoresis buffers maintain consistent pH levels during DNA, RNA, and other protein separation. Providing ions that transfer the applied current, the low charge solutions facilitate different molecular sizes to travel at various rates for analyses. Offering additional protection to proteins, the preparative substances prevent degradation for guaranteed sample viability and accurate testing. Available in various molecular weights, the best electrophoresis buffer can be selected based on the specific macromolecules being handled.
Buffer, SSC solution 20X concentrate (saline-sodium citrate)
Supplier: G-Biosciences
Hybridisation Buffer, SSC buffer solution 20X concentrate (saline-sodium citrate)
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IHC Wash Buffer, Enzo Life Sciences
Supplier: ENZO LIFE SCIENCES
The IHC wash buffer is a for washing and removing excess reagents during immunostaining.
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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer)
Supplier: BIOMOL RESEARCH LABORATORIES
Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)
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6X GelRed prestain loading buffer with tracking dye
Supplier: Biotium
6X GelRed prestain loading buffers are gel loading buffers containing density agents, tracking dyes, and GelRed dye. GelRed is a sensitive, stable and environmentally safe fluorescent nucleic acid dyes designed to replace the highly toxic ethidium bromide (EtBr).
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SSC buffer, 20X concentrate, pH 7,0
Supplier: Rockland Immunochemicals
Saline-sodium citrate (SSC) buffer is most commonly used as a buffer for the hybridisation process and washing in experiments where spcies of nucleic or ribonucleic acid are to be matched to complimentary sequences (such as Southern and Nouthern blotting, in situ hybridisation, or DNA microarrays). SSC is used to help control the stringency of hybridisation in these steps.
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PBS tablets
Supplier: PanReac AppliChem
Tablets for preparation of 100 ml phosphate buffered saline (PBS).
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Tween-Tris-buffered saline (TTBS), 10X concentrate, pH 7,5
Supplier: Rockland Immunochemicals
Tween Tris buffered saline is suitable for multiple applications including biological diluent buffer for antibodies or other biologics. Also may be used as a wash buffer for immunological assays including western blot, immunohistochemistry, immunofluorescence microscopy, and ELISA.
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In-situ hybridisation buffer (1.25X concentrate), Enzo Life Sciences
Supplier: ENZO LIFE SCIENCES
In Situ Hybridization Buffer provides a high quality hybridization medium for in situ hybridization analyses using either DNA or oligonucleotide probes labeled with biotin-, digoxigenin- or fluorescein-modified nucleotides.
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Buffer, PBS (10X, Dulbecco's) powder pack
Supplier: PanReac AppliChem
Powder mixture to prepare PBS buffer (10X) according to Dulbecco.
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WB blocking buffer kit, TrueBlack®
Supplier: Biotium
The TrueBlack® WB blocking buffer kit is a ready-to-use buffer system that provides optimal specificity, sensitivity, and background signal suppression for fluorescence-based western blotting (WB).
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Buffer, TRIS-glycine-SDS solution 10X concentrate (TGS buffer)
Supplier: BIOMOL RESEARCH LABORATORIES
Running Buffer, TRIS-glycine-SDS buffer solution 10X concentrate (TGS buffer)
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Buffer, TAE solution 50X concentrate (TRIS-acetate-EDTA buffer)
Supplier: BIOMOL RESEARCH LABORATORIES
Running Buffer, TAE buffer solution 50X concentrate (TRIS-acetate-EDTA buffer)
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SDS-PAGE Sample Buffer, 4X, (2-Mercaptoethanol-free) for Electrophoresis
Supplier: TCI
This product contains 8% SDS, 40% glycerol, 0.02% bromophenol blue and 0.25 M Tris HCl, pH approx. 6.8. Does not contain 2-Mercaptoethanol.
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Protein blocking agent, BLOT-QuickBlocker™
Supplier: G-Biosciences
BLOT-QuickBlocker™ is a modified milk protein that is highly soluble and does not inhibit peroxidase detection. The modified milk protein has high blocking efficiency with a clear background.
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Protein blocking buffers, Superior™
Supplier: G-Biosciences
Blocking buffer is an enhanced blocking agent available in multiple formats. Superior™ Blocking Buffer contains a proprietary antigenically non-determinant protein for blocking Western blot membranes, blocking and storage of ELISA plates, and blocking prior to immunohistochemistry staining.
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Buffer, TBE solution 5X concentrate (TRIS-borate-EDTA buffer)
Supplier: Biotium
Running Buffer, TBE buffer solution 5X concentrate (TRIS-borate-EDTA buffer)
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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer)
Supplier: Rockland Immunochemicals
Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)
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SDS-Page running gel buffers, 10X concentrate
Supplier: Rockland Immunochemicals
SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis, describes a technique widely used in biochemistry to separate proteins according to their electrophoretic mobility (a function of the length of a polypeptide chain and its charge) and no other physical feature. SDS is an anionic detergent applied to protein sample to linearise proteins and to impart a negative charge to linearised proteins.
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Buffer, TBS solution 10X concentrate (TRIS buffered saline)
Supplier: G-Biosciences
The TBS solution is primarily used as a wash and blocking buffer diluent for Western blot, ELISA and immunoassays.
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SSPE Buffer, 20X concentrate, pH 7,4
Supplier: Rockland Immunochemicals
20X SSPE buffer (3,0 M sodium chloride, 0,2 M sodium hydrogen phosphate, 0,02 M EDTA, pH 7,4) - DEPC treated.
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Buffer, TBS solution 10X concentrate (TRIS buffered saline) with phenol red, pH 7,4
Supplier: Cayman Chemical
250 mM Tris-base, pH 7,4, 1,37 M sodium chloride, containing 27 mM KCl, 0,4 mM phenol red.
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In-situ hybridisation buffer for HPV probes
Supplier: ENZO LIFE SCIENCES
In situ hybridisation buffer for HPV probes (ready-to use) provides a high quality hybridisation medium for In situ hybridisation analyses using either DNA or oligonucleotide probes labeled with biotin-, or fluorescein-modified nucleotides.
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Buffer, SSC solution 20X concentrate (saline-sodium citrate)
Supplier: G-Biosciences
Hybridisation Buffer, SSC buffer solution 20X concentrate (saline-sodium citrate)
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Buffer, TBE (Tris-borate-EDTA) powder for 10X concentrated solution
Supplier: PanReac AppliChem
Powder for 10X concentrated aqueous solution (170,28 g/L). Composed of 0,89 M Tris, 0,89 M boric acid and 0,02 M EDTA.Na₂.
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Blocker™ Blocking Buffer, BLOTTO in TBS
Supplier: Thermo Fisher Scientific
Blocker™ BLOTTO is a ready-to-use 5% solution of non fat powdered milk in Tris-buffered saline for Western blot, ELISA, IHC and other detection methods.
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Tris-Borate-EDTA (TBE) buffer, 10X, Molecular biology grade
Supplier: PanReac AppliChem
10X geconcenteerde oplossing van 0,89 M tris, 0,89 M boorzuur en 0,02 M EDTA.Na₂, pH 8,3±0,2.
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Blocking solution, BLOK™ BSA
Supplier: G-Biosciences
BLOK™ BSA is a 10% BSA protein blocking solution.
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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer)
Supplier: Thermo Fisher Scientific
Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)
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Buffer, TBE solution 5X concentrate (TRIS-borate-EDTA buffer)
Supplier: PanReac AppliChem
Running Buffer, TBE buffer solution 5X concentrate (TRIS-borate-EDTA buffer)
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Buffer, TAE solution 50X concentrate (TRIS-acetate-EDTA buffer)
Supplier: OMEGA BIO-TEK
Running Buffer, TAE buffer solution 50X concentrate (TRIS-acetate-EDTA buffer)