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Buffers voor elektroforese

The electrophoresis buffers maintain consistent pH levels during DNA, RNA, and other protein separation. Providing ions that transfer the applied current, the low charge solutions facilitate different molecular sizes to travel at various rates for analyses. Offering additional protection to proteins, the preparative substances prevent degradation for guaranteed sample viability and accurate testing. Available in various molecular weights, the best electrophoresis buffer can be selected based on the specific macromolecules being handled.

VWR® Transfer Buffer Concentrate, CAPS 10X

VWR® Transfer Buffer Concentrate, CAPS 10X

Supplier: VWR Chemicals

For transfer of protein to membranes for sequencing or blotting.

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VWR® Gentle ReView™, Stripping Buffer

VWR® Gentle ReView™, Stripping Buffer

Supplier: VWR Chemicals

Gentle ReView™ Stripping Buffer is a mild, yet effective method for stripping primary and secondary antibodies from PVDF or nitrocellulose membranes.

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Snelle blotbuffer, 10x

Snelle blotbuffer, 10x

Supplier: VWR Chemicals

Snelle blotbuffer is een eenvoudig ééncomponentsysteem voor snelle en efficiënte overdracht van eiwitten van SDS-PAGE-gels naar membranen voor Western blotting-toepassingen. De overdracht wordt voltooid in 10 of 20 minuten met behulp van een standaardapparaat voor respectievelijk semidry of nat overbrengen.

    
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Protein Transfer buffers

Supplier: G-Biosciences

Efficient™ Western Transfer Buffer (20X) achieves increased protein transfer efficiency. This ready-to-use transfer buffer is prepared for optimal conductivity and efficient protein transfer, without generating excessive heat or transfer distortion.

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VWR® Proteomics Grade, Tris-glycine buffer

VWR® Proteomics Grade, Tris-glycine buffer

Supplier: VWR Chemicals

A pre-blended Tris-glycine powder for preparation of Laemmli SDS-PAGE running buffer. The 40 litre pack size prepares 40 litres of 1X Tris-glycine solution (0,025 M Tris base and 0,192 M glycine). Each Ready-Pack™ prepares 1 litre of a 10X concentrate.

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Tris-Glycine buffer

Supplier: EDVOTEK

For vertical polyacrylamide gel electrophoresis.

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Western Blot Transfer buffer, 10X, methanol-free, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Methanol-Free 10X Western Blot Transfer Buffer is a space-saving stock solution for preparing the methanol-free transfer buffer used with cooled, tank (wet) western blotting transfer apparatus.

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Buffer, TRIS-glycine solution 10X concentrate (TG buffer), Pierce™

Buffer, TRIS-glycine solution 10X concentrate (TG buffer), Pierce™

Supplier: Thermo Fisher Scientific

Our variety of Pierce Concentrated Buffer stock solutions are ready to use without having to weigh and dissolve dry ingredients or adjust the pH with concentrated acid or base. Simply dilute the liquid stock solution with pure water and proceed with your experiment.

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Stripping buffer-2 (4X)

Supplier: Thermo Fisher Scientific

Buffer voor elektroforese, Stripping buffer, 4X

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Stripping buffer (4X)

Supplier: Thermo Fisher Scientific

Buffer voor elektroforese, Stripping buffer, 4X

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Stacking buffer (4X)

Supplier: Thermo Fisher Scientific

Buffer voor elektroforese, Stacking Buffer, 4X

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Nitrocellulose stripping buffer (10X)

Supplier: Thermo Fisher Scientific

Buffer voor elektroforese, Nitrocellulose Stripping Buffer, 10X

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Transfer or Electro Blotting buffer (10X), pH 8,5

Supplier: Thermo Fisher Scientific

Buffer voor elektroforese, Transfer buffer, Transfer Electroblot Buffer 10X

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Semi Dry Blot Transfer buffer (10X)

Supplier: Thermo Fisher Scientific

Buffer voor elektroforese, Transfer buffer, Semi-dry Blot Transfer Buffer, 10X

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Transfer buffer, Tris-glycine,10X, AccuGENE™

Transfer buffer, Tris-glycine,10X, AccuGENE™

Supplier: LONZA

AccuGENE™ electrophoresis buffers are ready-to-use solutions ideal for a wide range of molecular biology applications.

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Buffer, TRIS-glycine solution 10X concentrate (TG buffer)

Supplier: BIO RAD

Buffer voor elektroforese, Transfer buffer, TRIS-glycine buffer solution 10X concentrate (TG buffer)

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Phosphate Buffer, pH 6.6 at 25 °C

Supplier: SIGMA ALDRICH MICROSCOPY

Phosphate buffer's buffering capacity is pH sensitive and is preferred to be used at physiological pH (7,2 to 7,4).

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VWR®, Tris-borate-EDTA (TBE) buffer

VWR®, Tris-borate-EDTA (TBE) buffer

Supplier: VWR Chemicals

TBE is optimised for DNA electrophoresis techniques using both acrylamide and agarose. Buffers are available as liquid concentrate or Ready-Pack™ (a foil pouch containing sufficient material to prepare 1 litre of 10X concentrate).

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VWR® Buffer, MOPS-SDS, 20X, UltraPure Grade

VWR® Buffer, MOPS-SDS, 20X, UltraPure Grade

Supplier: VWR Chemicals

Useful for high resolution of proteins on neutral pH SDS-PAGE gels.

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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer), Millipore®

Supplier: Merck

Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)

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VWR® UltraPure, PBS and TBS wash buffers

VWR® UltraPure, PBS and TBS wash buffers

Supplier: VWR Chemicals

Wash buffers ideal for use in applications such as Western blotting and ELISA. Prevents high background by effectively removing unbound reagents.

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VWR® UltraPure, Buffer, concentrate (phosphate buffered saline)

VWR® UltraPure, Buffer, concentrate (phosphate buffered saline)

Supplier: VWR Chemicals

Washing buffer, PBS buffer solution 10X concentrate (phosphate buffered saline)

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VWR®, MOPS 10X buffer for biotechnology

VWR®, MOPS 10X buffer for biotechnology

Supplier: VWR Chemicals

MOPS is a zwitterionic buffer used as a running buffer for denaturing agarose gel electrophoresis of RNA. Having a pH buffering range from 6,5 to 7,9, MOPS works exceptionally well with formaldehyde gels at 20 mM concentration.

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VWR® TBE Buffer Solution, 10X Concentrate (Tris-Borate-EDTA Buffer), UltraPure Grade

VWR® TBE Buffer Solution, 10X Concentrate (Tris-Borate-EDTA Buffer), UltraPure Grade

Supplier: VWR Chemicals

Running Buffer, TBE buffer solution 10X concentrate (Tris-borate-EDTA buffer)

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VWR® UltraPure, Tris-glycine buffers, 10X concentrate

VWR® UltraPure, Tris-glycine buffers, 10X concentrate

Supplier: VWR Chemicals

Running buffers for both 1-D and 2-D protein gel electrophoresis.

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VWR® Buffer, MES-SDS, 20X, UltraPure Grade

VWR® Buffer, MES-SDS, 20X, UltraPure Grade

Supplier: VWR Chemicals

Useful for high resolution of proteins on neutral pH SDS-PAGE gels.

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Fosfaatgebufferde zoutoplossing (Phosphate Buffered Saline, PBS), laag fosfaatgehalte

Fosfaatgebufferde zoutoplossing (Phosphate Buffered Saline, PBS), laag fosfaatgehalte

Supplier: VWR Chemicals

Geïrradieerde oplossing voor gebruik bij het wassen van cellen.

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VWR® UltraPure, Tris-acetate-EDTA (TAE) buffer

VWR® UltraPure, Tris-acetate-EDTA (TAE) buffer

Supplier: VWR Chemicals

TAE is an extensively used buffer for agarose gel electrophoresis applications requiring high resolution and separation of high molecular weight, double-stranded DNA. TAE buffer is more compatible with in-gel manipulations and band recovery procedures than TBE buffer.

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RapidBlock™ oplossing, 10x

RapidBlock™ oplossing, 10x

Supplier: VWR Chemicals

RapidBlock™ oplossing vermindert de tijd tot 5 minuten voor Western- en dot blottingprocedures op PVDF en nitrocellulosemembranen. Deze proteïnevrije samenstelling minimaliseert cross-reactiviteit en niet-specifieke antilichaambinding, voor het genereren van blots met lage achtergrond en verbeterde signaal-ruisverhouding.

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VWR®, Blocking buffer, non-fat powdered milk

VWR®, Blocking buffer, non-fat powdered milk

Supplier: VWR Chemicals

Each pack of powdered blocking buffer makes one liter of 1X solution.

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