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Protein purification systems are intended to isolate one or multiple proteins from a complex mixture. Working with cells, tissues, or whole organisms, the machines purify with predefined start points and editable method templates. With a single click, the compact designed equipment offers real-time run control and visualization. Suitable for even cold-room operations, the products enable you to perform purification of affinity-tagged proteins, antibodies, untagged or native proteins, as well as sample clean-up.
Gel filtration media, Sephadex™ G-15
Supplier: Cytiva
Sephadex™ G-15 is a well established gel filtration medium for desalting and buffer exchange of peptides and small biomolecules >1500 molecular weight.
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Accessories for chromatography systems
Supplier: Cytiva
UniTag sheet with 108 labels
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Affinity chromatography media, IMAC Sepharose™ 6 Fast Flow
Supplier: Cytiva
IMAC Sepharose 6 Fast Flow is composed of cross-linked 6% agarose beads modified with a novel chelating ligand immobilized to the base matrix.
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Affinity chromatography media, Capto™ Blue
Supplier: Cytiva
Capto™ Blue is an affinity medium for the capture of albumin, purification of enzymes, and recombinant proteins at laboratory and process scales.
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Ion exchange chromatography media, Capto™ S ImpAct
Supplier: Cytiva
Capto™ S ImpAct is a strong cation exchange chromatography medium (resin) developed for polishing of monoclonal antibodies (MAbs) and other biomolecules.
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Ion exchange chromatography media, CM Sepharose™ Fast Flow
Supplier: Cytiva
CM Sepharose™ Fast Flow is a weak cation exchanger based on the well established Sepharose™ Fast Flow ion exchange platform, extensively used for preparative protein separations in both research and industrial applications.
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Affinity coupling media, CNBr-activated Sepharose™ 4
Supplier: Cytiva
CNBr-activated Sepharose™ 4 Fast Flow is based on the Sepharose™ Fast Flow platform. The resin is composed of cross-linked 4% agarose beads that has been pre-activated with cyanogen bromide.
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Toebehoren en reserveonderdelen voor ÄKTApilot
Supplier: Cytiva
Y connector, 3×UNF 5/16" female
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SP Sepharose™ XL Ion Exchange Chromatography Media
Supplier: Cytiva
SP Sepharose™ XL is a strong cation exchanger designed for use in the capture steps of biomolecules from clarified feed-stocks.
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Superose™ 6 Gel Filtration Media, Prep Grade
Supplier: Cytiva
Superose™ 6 PG for separations of proteins and other biomolecules according to size. Wide separation in two molecular weight ranges from 1000 up to 5 million.
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SDS-PAGE Clean Up Kit
Supplier: Cytiva
SDS-PAGE clean up kit is designed to prepare samples for SDS-PAGE that are frequently difficult to analyse due to the presence of salts or low protein concentration.
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Affinity chromatography media, MabSelect SuRe™ LX
Supplier: Cytiva
MabSelect SuRe™ LX is a protein A affinity medium with very high dynamic binding capacity at extended residence times and is developed for high titer antibody processes.
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Gel filtration media, Sephacryl™ High Resolution
Supplier: Cytiva
Sephacryl™ High Resolution (HR) gel filtration media allow fast and reproducible purification of proteins, polysaccharides, and other macromolecules by gel filtration at laboratory and industrial scale.
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Ion exchange chromatography media, SOURCE™ 15Q
Supplier: Cytiva
Mono-sized 15 um rigid beads gives low back pressure and high flow rates allowing for high productivity.
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Gel filtration media, Sephadex™ G-100
Supplier: Cytiva
Sephadex™ G-100 is useful for desalting and buffer exchange of very large biomolecules. Sephadex™ G-100 Superfine is designed for specific cases requiring very high resolution.
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Reversed phase chromatography media, SOURCE™ 15RPC
Supplier: Cytiva
SOURCE™ 15RPC is designed for reversed phase chromatography (RPC) with full pH range stability and suitable for polishing steps in industrial processes requiring high flow velocities and low back-pressure.
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Hydrophobic interaction chromatography media, Capto™ Butyl
Supplier: Cytiva
Capto™ Butyl is used in the capture and intermediate stages of protein purification. The combination of high capacity, high flow rate and low back pressure reduces process cycle times.
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Multimodal chromatography media, Capto™ adhere
Supplier: Cytiva
Capto™ adhere is a multimodal anion exchanger for intermediate purification and polishing of monoclonal antibodies after the Protein A capture step, giving the possibility to design a two-step chromatographic downstream process.
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Q Sepharose™ XL Ion Exchange Chromatography Media, Cytiva
Supplier: Cytiva
Q Sepharose XL is based on a highly cross-linked, bead formed 6% agarose matrix similar to the well established Q Sepharose Fast Flow media.
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Affinity purification media, rProtein A Sepharose™ Fast Flow
Supplier: Cytiva
rProtein A Sepharose™ 4 Fast Flow is a well established antibody affinity medium is designed for the purification of monoclonal and polyclonal antibodies based on Sepharose™ Fast Flow platform. The recombinant protein A is produced in E.coli and engineered for an oriented coupling to giving a matrix with enhanced binding capacity. The epoxy-based coupling ensures low ligand leakage. The specificity of the recombinant protein A for the Fc region of IgG is similar to native protein A and provides excellent purification in one step. The high capacity, low ligand leakage and a well established base matrix makes rProtein A Sepharose™ Fast Flow ideal for purification of monoclonal antibodies at both laboratory and process scale.
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Ion exchange chromatography media, Capto™ Q
Supplier: Cytiva
Capto™ Q is a strong anion exchange bioprocess medium, designed to meet the demands of modern large-scale manufacturers for fast, efficient, and cost-effective capture and intermediate protein purification when high volume throughput is essential.
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Ion exchange chromatography media, Capto™ S
Supplier: Cytiva
Capto™ S is a strong cation exchange bioprocessing medium, designed to meet the demands of modern large-scale manufacturers for fast, efficient, and cost-effective capture and intermediate protein purification when high volume throughput is essential.
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Biacore consumables and accessories
Supplier: Cytiva
Biacore maintenance kits are convenient kits for proper instrument maintenance.
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Sepharose Chromatography Resins, Strep-Tactin® XT
Supplier: Cytiva
A chromatography resin for purification of recombinant proteins tagged with Strep-tag II and Twin-Strep-tag. These proteins bind very specifically to the immobilised Strep-Tactin XT ligand giving highly pure target protein.
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Affinity chromatography media, Streptavidin Sepharose™ High Performance
Supplier: Cytiva
Streptavidin Sepharose™ High Performance is an affinity chromatography resin designed for fast and reliable, high resolution purification of biotinylated biomolecules.
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Protein A from Staphylococcus aureus, MP Biomedicals
Supplier: MP Biomedicals
Protein A is a recombinant Fc-binding protein from Staphylococcus aureus, Cowan strain-1 gene. The product is a carboxy truncated material containing 301 amino acid residues. The non-binding regions have been removed to minimise potenial steric interference.
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Affinity chromatography media, Glutathione Sepharose™ High Performance
Supplier: Cytiva
Glutathione Sepharose High Performance is used for the purification of GST-tagged proteins from bacterial lysates and other S-transferases or glutathione-dependent proteins.
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Sepharose™ CL-4B Octyl Hydrophobic Interaction Chromatography Media
Supplier: Cytiva
Octyl-Sepharose™ CL-4B is a well-proven medium for hydrophobic interaction chromatography (HIC). Octyl immobilised on 4% cross-linked agarose.
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Calmodulin Sepharose™ 4B Affinity Chromatography Media, Cytiva
Supplier: Cytiva
Calmodulin Sepharose™ 4B is calmodulin immobilised by the CNBr method to Sepharose™ 4B. Calmodulin is a highly conserved regulatory protein found in all eukaryotic cells. This protein is involved in many cellular processes such as glycogen metabolism, cytoskeletal control, neurotransmission, phosphate activity and control of NAD⁺/NADP⁺.
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Affinity chromatography media, Heparin Sepharose™ 6 Fast Flow
Supplier: Cytiva
Heparin Sepharose 6 Fast Flow is composed of cross-linked 6% agarose beads modified sodium heparin. Immobilization is performed using chemically stable and oriented coupling giving high binding capacity. The chemical stability of the media is solely dictated by the heparin ligand. Heparin Sepharose 6 Fast Flow is a member of the Cytiva range of affinity chromatography media for capture and intermediate purification.