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Eiwitzuivering

Eiwitzuivering

Eiwitzuiveringssystemen zijn bedoeld om één of meerdere eiwitten uit een complexe mix te isoleren. Ze werken met cellen, weefsels of complete organismen en zuiveren via vooraf gedefinieerde startpunten en bewerkbare methodesjablonen. Met één klik biedt de compact ontworpen apparatuur realtime runcontrole en visualisatie. Geschikt voor gebruik in koelkamers, maken de producten zuivering mogelijk van affiniteits-gelabelde eiwitten, antilichamen, ongelabelde of native eiwitten, evenals monstervoorzuivering.

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Recombinant protein A/G, Pierce™

Recombinant protein A/G, Pierce™

Supplier: Thermo Fisher Scientific

Purified (unconjugated) Pierce™ Recombinant Protein A/G is useful as the basis for preparing various kinds of probes or affinity media for detection or purification of rabbit and human antibodies, especially IgG isotypes, in immunoassays and antibody purification protocols.

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HisPur™ Ni-NTA spin purification kits

HisPur™ Ni-NTA spin purification kits

Supplier: Thermo Fisher Scientific

The HisPur™ Ni-NTA Spin Purification Kit provides a fast, easy to use format for routine affinity purification of His-tagged fusion proteins and contains 0,2-ml HisPur™ Ni-NTA spin columns, as well as buffers required to purify polyhistidine-tagged proteins.

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Coupling Resin, AminoLink™ Plus

Coupling Resin, AminoLink™ Plus

Supplier: Thermo Fisher Scientific

AminoLink™ Plus Coupling Resin uses aldehyde-activated agarose beads for high-yield covalent coupling of antibodies (proteins) via primary amines to prepare columns for affinity purification.

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Immobilised D-galactose, Pierce™

Immobilised D-galactose, Pierce™

Supplier: Thermo Fisher Scientific

Immobilised D-Galactose agarose enables purification of lectins, galactosidases and other galactose-binding molecules. The immobilised D-galactose is a beaded agarose affinity resin that enables purification of agglutinins, lectins, toxins, and other galactose-binding proteins, N-acetylgalactosamine-binding proteins or carbohydrate-binding proteins.

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Pierce™, CarboxyLink™ Immobilisation Kit

Pierce™, CarboxyLink™ Immobilisation Kit

Supplier: Thermo Fisher Scientific

CarboxyLink™ immobilisation kit is for covalent immobilisation of peptides or other carboxyl-containing (—COOH) molecules to a porous, beaded resin for use in affinity purification procedures.

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Recombinant protein G, Pierce™

Recombinant protein G, Pierce™

Supplier: Thermo Fisher Scientific

Purified (unconjugated) Pierce™ Recombinant Protein G is useful as the basis for preparing various kinds of probes or affinity media for detection or purification of rabbit and human antibodies, especially IgG isotypes, in immunoassays and antibody purification protocols.

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Protein IgG Plus orientation kits, Pierce™

Protein IgG Plus orientation kits, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Protein IgG Plus Orientation Kits uses a crosslinker to covalently attach antibody that is bound to Protein A (or Protein G) agarose resin, thereby creating a reusable affinity column for purification of antigens.

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c-Myc Tag IP/Co-IP Kit, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ c-Myc Tag IP/Co-IP Kit provides a simple and fast method to study c-Myc-tagged proteins, with advantages over the traditional IP procedure using protein A/G agarose.

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Immobilised GST agarose, Pierce™

Immobilised GST agarose, Pierce™

Supplier: Thermo Fisher Scientific

Immobilised GST is glutathione S-transferase from Schistosoma japonicum that has been covalently immobilised on crosslinked 6% beaded agarose.

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SulfoLink™ Coupling Resin

SulfoLink™ Coupling Resin

Supplier: Thermo Fisher Scientific

SulfoLink™ coupling resin is porous, crosslinked, 6% beaded agarose that has been activated with iodoacetyl groups for covalent immobilisation of cysteine-peptides and other sulfhydryl molecules.

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Pierce™, UltraLink™ Affinity Chromatography Resin, Immobilised Protein A/G

Pierce™, UltraLink™ Affinity Chromatography Resin, Immobilised Protein A/G

Supplier: Thermo Fisher Scientific

Pierce™ Protein A/G UltraLink™ and Protein A/G Plus UltraLink™ resins are extremely durable beaded polyacrylamide supports to which recombinant Protein A/G has been immobilised for use in IgG purification methods. Immoblised proteins are covalently coupled to an agarose support to provide an efficient method of purifying antibodies.

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Immobilised jacalin agarose, Pierce™

Immobilised jacalin agarose, Pierce™

Supplier: Thermo Fisher Scientific

Jacalin is an alpha-D-galactose binding lectin extracted from jack-fruit seeds (Artocarpus integrifolia). This lectin is a glycoprotein (approx. 40 kDa) composed of four identical subunits. Jacalin binds to human IgA1 (also called serum or secretory IgA) with high specificity (i.e., it does not bind IgG or IgM). Thus, Jacalin that has been immobilised on crosslinked beaded agarose is useful for purifying human IgA from serum or colostrum samples.

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Melon™ Gel IgG Purification Kit

Melon™ Gel IgG Purification Kit

Supplier: Thermo Fisher Scientific

The Melon Gel IgG purification kit offers a simpler and faster alternative to traditional Protein A and Protein G methods for purification of human, mouse and rabbit IgG from serum and other sources.

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Affinity chromatography resin, CDI-activated agarose, Pierce™

Affinity chromatography resin, CDI-activated agarose, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ CDI-activated agarose is carbonyldiimidazole affinity chromatography resin, activated for covalent immobilisation of N-nucleophiles and primary amine ligands in aqueous or organic solvent conditions.

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Streptavidin HRP conjugates, high sensitivity, Pierce™

Streptavidin HRP conjugates, high sensitivity, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ High Sensitivity Streptavidin-HRP is a peroxidase-conjugated streptavidin biotin-binding protein that provides signal amplification like poly-HRP and exceptional storage stability.

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Immobilisation kit, AminoLink™ Plus, Pierce™

Immobilisation kit, AminoLink™ Plus, Pierce™

Supplier: Thermo Fisher Scientific

AminoLink™ Plus immobilisation kit contains AminoLink™ Plus coupling resin and additional reagents to perform five protein immobilisations, with expected yields of 2 to 40 mg protein per column. The coupling resin uses aldehyde-activated agarose beads for high-yield covalent coupling of antibodies (proteins) via primary amines to prepare columns for affinity purification.

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