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Supplier: Merck

The Amicon® Pro purification system - A centrifugal tool collapsing protein purifcation with concentration and buffer exchange.

Supplier: ImmunoReagents

ImmunoReagents Chicken IgY Affinity Prep resins are prepared using highly purified species specific immunoglobulins. This resin is suitable for removal of proteins recognising Chicken IgY from liquid solutions, including traditional Immunoabsorptions and Immunoprecipitation protocols.

Supplier: G-Biosciences

STRATEGY™ is designed to allow researchers to rapidly screen a number of purification techniques in a small scale format to identify the system to isolate their protein of interest. The self-contained kit allows researchers to save time and money as separate buffers, resins and columns are not needed.

Supplier: Rockland Immunochemicals

Protein A Sepharose™ is a suspension of beads conjugated to native Protein A derived from Staphylococcus aureaus. Protein A has a high affinity for the Fc regions of IgG molecules from a variety of species, including human, mouse, rat IgG2c, cow IgG2c, goat IgG2, sheep IgG2 and rabbit.

Supplier: G-Biosciences

The Pearl™ IgG purification kits are designed for the one step purification of IgG antibodies from serum. The supplied Pearl™ IgG purification resin binds the high abundant, non-IgG proteins (i.e. albumin) and allows the IgG molecules to pass through in a physiological buffer. The purified IgG molecules can be stored or used in further downstream applications without further clean-up, such as ammonium sulphate precipitation.

Supplier: G-Biosciences

The SpinOUT™ GT-100, GT-600 and GT-1200 columns are versatile, spin format columns for the desalting and buffer exchange of protein solutions ranging from 5 µl through to 4 ml sample volumes. The SpinOUT™ columns are available in three MWCO sizes for >700, >6000 or >30 000 dalton proteins and are suitable for samples containing as little as 20 µg protein/ml.

Supplier: G-Biosciences

Following digestion of proteins with proteases, the addition of Pep-Extract™ rapidly elutes digested peptides in under 20 minutes. The eluted peptides can be used directly in mass spectrometry analysis without any additional clean up. Contains sufficient for 500 protein spots.

Supplier: AGILENT

Starter reagent kit for multiple affinity removal LC columns.

Supplier: ImmunoReagents

ImmunoReagents Bovine IgG Affinity Prep resins are prepared using highly purified species specific immunoglobulins. This resin is suitable for removal of proteins recognising Bovine IgG from liquid solutions, including traditional Immunoabsorptions and Immunoprecipitation protocols.

Supplier: ImmunoReagents

ImmunoReagents Donkey IgG Affinity Prep resins are prepared using highly purified species specific immunoglobulins. This resin is suitable for removal of proteins recognising Donkey IgG from liquid solutions, including traditional Immunoabsorptions and Immunoprecipitation protocols.

Supplier: G-Biosciences

The Pearl™ Monoclonal IgG Purification Kit allows for the rapid purification of antibodies from cell culture supernatant and ascites fluid. The Pearl™ IgG purification resin binds the high abundant, non-IgG proteins (i.e. albumin) and allows the IgG molecules to pass through in a physiological buffer. The IgG molecules can be stored or used in downstream applications without further clean-up, such as ammonium sulphate precipitation.

Supplier: G-Biosciences

The Pearl™ Antibody Clean Up Kit is designed for the rapid clean up of antibody solutions using a combination of our Pearl™ IgG Purification Resin to remove the protein stabilisers and SpinOUT™ desalting columns to ensure the antibody solutions are in an optimal buffer for clean up. It binds high abundant, non IgG proteins (i.e. BSA and gelatin) and allows the IgG molecules to pass through in a physiological buffer. For the purification of ten 0,5 ml IgG samples with up to 1% BSA and gelatin.

Supplier: G-Biosciences

Concanavalin A (Con A) agarose consists of Con A coupled to 6% agarose by the cyanogen bromide method. Con A is a tetrameric metalloprotein lectin isolated from Canavalia ensiformis (jack bean). It is used for the purification of glycoproteins, polysaccharides and glycolipids as it binds molecules containing α-D-mannopyranosyl, α-D-glucopyranosyl and sterically related residues. It has also be used in other application areas including purification of enzyme-antibody conjugates, purification of IgM and separation of membrane vesicles. As stated above, it is a metalloprotein and to maintain its binding characteristics the presence of both Mn²⁺ and Ca²⁺ is essential. Each subunit of Con A utilises one calcium and one manganese ion and these cations can be removed under acidic conditions abolishing the carbohydrate-binding activity.

Supplier: Genscript

Anti-His affinity resins are designed for purification of His-tagged protein. Composed of agarose and conjugated THE™ anti-His monoclonal antibody, they are compatible with most commonly used reagents at certain concentrations.

Supplier: ImmunoReagents

ImmunoReagents Goat IgG Affinity Prep resins are prepared using highly purified species specific immunoglobulins. This resin is suitable for removal of proteins recognising Goat IgG from liquid solutions, including traditional Immunoabsorptions and Immunoprecipitation protocols.

Supplier: Thermo Fisher Scientific

AminoLink™ Plus Coupling Resin uses aldehyde-activated agarose beads for high-yield covalent coupling of antibodies (proteins) via primary amines to prepare columns for affinity purification.

Supplier: Thermo Fisher Scientific

The HisPur™ Ni-NTA Spin Purification Kit provides a fast, easy to use format for routine affinity purification of His-tagged fusion proteins and contains 0,2-ml HisPur™ Ni-NTA spin columns, as well as buffers required to purify polyhistidine-tagged proteins.

Supplier: MP Biomedicals

The FastPROTEIN™ Blue Matrix Kit (also known as Lysing Matrix B) is designed for rapid isolation and purification of protein from bacterial cultures.

Supplier: Thermo Fisher Scientific

Purified (unconjugated) Pierce™ Recombinant Protein A/G is useful as the basis for preparing various kinds of probes or affinity media for detection or purification of rabbit and human antibodies, especially IgG isotypes, in immunoassays and antibody purification protocols.

Supplier: Thermo Fisher Scientific

The Pierce™ Magnetic ChIP Kit provides a convenient method for efficient isolation of chromatin-bound DNA by immunoprecipitation for subsequent quantitation by PCR.

Supplier: Thermo Fisher Scientific

Pierce™ Glutathione Superflow Agarose consists of glutathione immobilised to a crosslinked 6% beaded agarose for purification of GST-tagged proteins.

Supplier: Thermo Fisher Scientific

Pierce™ protein G plus agarose is a high capacity protein A beaded agarose resin for use in a variety of antibody affinity purification methods.

Supplier: Thermo Fisher Scientific

NeutrAvidin™ UltraLink™ resin is a beaded polyacrylamide resin of immobilised NeutrAvidin™ Protein, a modified form of avidin with exceptional biotin-binding characteristics for affinity purification methods.

Supplier: Thermo Fisher Scientific

UltraLink™ Hydrazide resin is used to prepare affinity columns with antibodies or other glycolsylated proteins.

Supplier: Thermo Fisher Scientific

CarboxyLink™ immobilisation kit is for covalent immobilisation of peptides or other carboxyl-containing (—COOH) molecules to a porous, beaded resin for use in affinity purification procedures.

Supplier: Thermo Fisher Scientific

Pierce™ Protein L Plus agarose is a high-capacity Protein L beaded agarose resin for use in a variety of antibody affinity purification methods.

Supplier: Thermo Fisher Scientific

Pierce™ Protein A agarose is a standard-capacity Protein A beaded agarose resin for use in a variety of antibody affinity purification methods.

Supplier: Thermo Fisher Scientific

Immobilised D-Galactose agarose enables purification of lectins, galactosidases and other galactose-binding molecules. The immobilised D-galactose is a beaded agarose affinity resin that enables purification of agglutinins, lectins, toxins, and other galactose-binding proteins, N-acetylgalactosamine-binding proteins or carbohydrate-binding proteins.

Supplier: Thermo Fisher Scientific

Thermo Scientific™ Maleimide Activated NeutrAvidin™ Protein is a specially-prepared form of avidin that decreases background in biotin-binding.

Supplier: Thermo Fisher Scientific

UltraLink™ Biosupport is a durable, azlactone-activated, beaded-polyacrylamide resin to covalently immobilise proteins and other primary amine biomolecules for preparation of affinity purification columns.