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Proteïne-assays

Proteïne-assays

Een nauwkeurige kwantificatie van de totale eiwitconcentratie is essentieel voor de meeste experimenten en vaak noodzakelijk voor de isolatie, scheiding en analyse van eiwitten met biochemische methoden. Wanneer het nodig is om de totale eiwitconcentratie in een monster te bepalen, is een van de eerste factoren om te overwegen de keuze van een geschikte eiwitbepalingsmethode. Deze keuze is doorgaans gebaseerd op de compatibiliteit van de methode met de te analyseren monsters. Elke methode heeft zijn eigen voor- en nadelen.

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VWR® Protein Precipitation Kit

VWR® Protein Precipitation Kit

Supplier: VWR Chemicals

The Protein Precipitation Kit utilises a simple trichloroacetic acid (TCA) based method to precipitate proteins in complex mixtures. Only protein in the sample is pelleted, while interfering salts and surfactants remain in the supernatant. A gentle acetone wash removes TCA and co-precipitants allowing the end-user to recover the protein in a small volume of buffer.

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Bicinchoninic acid disodium salt hydrate

Supplier: SIGMA ALDRICH MICROSCOPY

Bicinchoninic acid is used for the determination of protein concentration. It is responsible for the making of intense purple complex with cuprous ion (Cu+) in the presence of alkaline conditions. The amount of Cu+ is in relation with the protein concentration.

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eFluxx-ID® multidrug resistance assay kit

Supplier: Enzo Life Sciences

The EFLUXX-ID® Multidrug Resistance Assay Kit allows for functional detection of all three clinically relevant ABC transporter proteins MDR1 (p-glycoprotein), MRP1/2, and BCRP. The assay uses a hydrophobic, non-fluorescent compound that readily penetrates the cell membrane, where it is hydrolyzed to a hydrophilic fluorescent dye by intracellular esterases.

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Conjugate stabilisers

Conjugate stabilisers

Supplier: Rockland Immunochemicals

Conjugate stabilisers are used with horseradish peroxidase (HRP) and calf intestinal alkaline phosphatase conjugated primary or secondary antibodies or other proteins or compounds in solution. The components of the stabiliser (proprietary) are unique and stabilise both the antibody and enzyme portions of the conjugate.

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Thermal shift protein stability kits, GloMelt™

Supplier: Biotium

Kit for fluorescence-based measurement of protein unfolding or thermal stability in thermal shift assay, also called Protein Thermal Shift™, differential scanning fluorimetry, or Thermofluor assay.

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Foxp3 / Transcription Factor Staining Buffer Kit, Tonbo Biosciences

Supplier: Tonbo Biosciences

Tonbo Biosciences Foxp3 / Transcription Factor Staining Buffer Kit contains specially formulated buffers and solutions for optimal resolution and low background in your analysis of nuclear antigens by flow cytometry

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Protein carbonyl colorimetric assay kit

Supplier: Cayman Chemical

The Protein Carbonyl Colorimetric Assay Kit is for the measurement of oxidised proteins. Protein samples are derivatised by making use of the reaction between 2,4-dinitrophenylhydrazine and protein carbonyls.

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Protein assay kits, RED 660™

Protein assay kits, RED 660™

Supplier: G-Biosciences

RED 660™ Protein Assay is a single reagent colorimetric assay that outperforms many commercial colorimetric assays, including Bradford and improved Coomassie/Bradford assays. It offers greater linearity, colour stability, and compatibility with detergents, reducing agents and other interfering agents compared to the Coomassie assays. The single, ready to use reagent allows for rapid analysis of total protein concentration and generates highly reproducible results.

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Bicinchoninic acid (BCA) reducing agent compatible protein Assay

Bicinchoninic acid (BCA) reducing agent compatible protein Assay

Supplier: G-Biosciences

The Bicinchoninic Acid (BCA) Protein Assay is a highly sensitive colorimetric assay that is compatible with detergent solubilised protein solutions, however is dramatically affected by the presence of reducing agents, including DTT, ß-mercaptoethanol and TCEP.

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Protein quantitation assay kits, Non-Interfering™ (NI™) Protein Assay

Protein quantitation assay kits, Non-Interfering™ (NI™) Protein Assay

Supplier: G-Biosciences

A highly sensitive colorimetric protein assay that overcomes interference of common laboratory agents present in protein solutions and shows minimal protein-to-protein variation. The assay is unaffected by the presence of common laboratory agents such as reducing agents, chelating agents, detergents, amines, sugars, chaotropes, salts, drugs, antibiotics, cobalt, and others.

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SUMO-QAPTURE-T® kit for isolation and enrichment of SUMOylated proteins

Supplier: Enzo Life Sciences

The only commercially available kit for isolation and enrichment of SUMOylated proteins.

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Protein Digestibility Assay Kit

Supplier: MEGAZYME

The Protein Digestibility Assay Kit (K-PDCAAS) is used for in vitro measurement of the Animal-Safe Accurate Protein Quality Score (ASAP-Quality Score Method).

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PROTEOSTAT® protein aggregation assay

PROTEOSTAT® protein aggregation assay

Supplier: Enzo Life Sciences

PROTEOSTAT® Protein aggregation assay provides a simple, homogenous assay format for monitoring peptide and protein aggregation in solution. This is useful for defining optimal storage formulations for proteins, for screening of compounds that promote or inhibit protein aggregation and potentially for the sensitive measurement of molecular chaperone activity. The assay can be employed to streamline protein processing and formulation optimization procedures.

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Glycogen Assay Kit

Glycogen Assay Kit

Supplier: Cayman Chemical

A simple and sensitive tool for assaying glycogen from tissue.

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L-Lactate Assay Kit

L-Lactate Assay Kit

Supplier: Cayman Chemical

A fluorescence-based method for detecting L-lactate in biological samples such as serum, plasma, blood, urine, and saliva.

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Bicinchoninic acid (BCA) protein assay

Bicinchoninic acid (BCA) protein assay

Supplier: G-Biosciences

The Bicinchoninic Acid (BCA) Protein Assay is a highly sensitive colorimetric assay that is compatible with detergent solubilised protein solutions.

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