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Cellular assays have always been a powerful tool in the research lab. They are incredibly versatile, and can be designed to measure virtually any cellular or biochemical function. Sensitive detection of fluorescent probes aid in evaluating cell signalling and death pathways, which is useful for the rapid quantification of cell viability. Cellular assays can be used to measure the effects of different treatments on cell viability and to evaluate toxic effects mediated through a variety of mechanisms.
MTT cell proliferation assay kit
Supplier: OZ BIOSCIENCES
MTT cell proliferation assay kit is designed for spectrophotometric quantification of cell growth, viability and proliferation and can be used as a direct indicator of cytotoxicity (such as for screening anticancer drugs) and apoptosis.
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Spexyte™ Intracellular pH Calibration Buffer Kit
Supplier: AAT Bioquest
Intracellular pH (pHi) plays an important modulating role in many cellular events, including cell volume regulation, cellular metabolism, calcium regulation, receptor-mediated signal transduction, ion transport, endocytosis, and other cellular processes.
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CytoScan™ LDH Cytotoxicity Assay kit
Supplier: G-Biosciences
CytoScan™ LDH Cytotoxicity assay quantitatively measures the cytosolic, lactate dehydrogenase (LDH) enzyme, which is released from damaged cells. The released LDH is measured with a coupled enzymatic reaction that results in the conversion of a tetrazolium salt (iodonitrotetrazolium; (INT)) into a red color formazan by diaphorase. The resulting formazan is measured at 490 nm.
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Amplite® Fluorimetric Sodium Ion Quantification Kit
Supplier: AAT Bioquest
Designed for consistent and dependable results. Amplite® fluorimetric sodium ion quantification kit uses our robust sodium ion indicator dye, SoNa™ 520, which exhibit great fluorescence intensity enhancement upon binding to sodium ions.
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Amplite® Fluorimetric Lithium Ion Quantification Kit
Supplier: AAT Bioquest
Designed for consistent and dependable results. Amplite® fluorimetric lithium ion quantification kit uses our new robust lithium-ion indicator dye, Lithiumighty™ 520, which exhibits great fluorescence intensity enhancement upon binding to lithium ions.
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ROS Detection assay kit
Supplier: OZ BIOSCIENCES
ROS Detection Assay Kit is a cell-based assay designed for measuring Reactive Oxygen Species (ROS) activity within a cell using the cell-permeable fluorogenic probe 2', 7'-Dichlorodihydrofluorescein diacetate (DCF-DA). Once DCF-DA has diffused into cells it is deacetylated by cellular esterases to a non-fluorescent compound and rapidly oxidised by ROS into DCF. DCF is highly fluorescent and can be detected by microscopy, titration in microplate and also cytometry (ex 485/em 535). The fluorescence intensity that results is proportional to the ROS levels within the cell.
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Amyloïde plaquekleuringsreagens, met EtBr-tegenkleuring, Amylo-Glo® RTD™
Supplier: Biosensis
Amylo-Glo RTD-reagens is ontworpen om amyloïde plaques in weefselcoupes te kleuren.
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Alamar blue cell viability assay
Supplier: G-Biosciences
AlamarBlue Cell Viability Assay reagent quantitatively measures the proliferation of mammalian cell lines, bacteria and fungi. The dye incorporates an oxidation-reduction (REDOX) indicator that both fluoresces and change colour in response to the chemical reduction of growth medium due to cell growth.
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Firefly luciferase assay kit 2.0
Supplier: Biotium
This kit is designed to measure Renilla luciferase activity in transfected cell reporter gene assays.
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Yeast viability staining kits
Supplier: Biotium
The Yeast Viability Staining Kit is a fluorescent assay to determine yeast viability. Each kit contains two fluorescent components: CF® Dye-labeled Concanavalin A (Con A) and Live-or-Dye™ viability dye. Biotium offers 3 different color combinations to choose from.
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BCA protein assay kit
Supplier: Merck Millipore (Novagen)
BCA Protein Assay is a simple and reliable protein quantification method. It can be used to determine protein concentration in the range of 20-2000 µg/ml in either a standard assay or microassay configuration.
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Firefly and Renilla Luciferase single tube Assay
Supplier: Biotium
This kit is designed to measure Firefly and Renilla luciferase activities in transfected cell reporter assays. Firefly luciferase is widely used as a reporter for studying gene regulation and function, and for pharmaceutical screening. It is a very sensitive genetic reporter due to the absence of endogenous luciferase activity in mammalian cells or tissues. Renilla luciferase has been used as a reporter gene for studying gene regulation and function in vitro and in vivo. It commonly is used in multiplex transcriptional reporter assays or as a normalising transfection control for firefly luciferase assays.
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Firefly luciferase assay kit
Supplier: Biotium
Firefly luciferase is widely used as a reporter for studying gene regulation and function, and for pharmaceutical screening. It is a very sensitive genetic reporter due to the absence of endogenous luciferase activity in mammalian cells or tissues.
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Fixable viability staining kits
Supplier: Biotium
Live-or-Dye™ Fixable Viability Staining Kits are designed for discrimination between live and dead cells during flow cytometry or microscopy.
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Live-or-Dye™ Yeast fixable live/dead staining kit
Supplier: Biotium
The Yeast Live-or-Dye™ Fixable Live/Dead Staining Kit is a fixable, fluorescent assay to determine yeast viability. The kit contains two fluorescent components, Thiazole Orange and Live-or-Dye™ 568/583.
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CellBrite™ Fix membrane stains, Biotium
Supplier: Biotium
CellBrite™ Fix dyes are fluorogenic membrane dyes that covalently stain the plasma membrane in live cells. They are unique among membrane stains in which they can withstand both fixation and detergent permeabilisation.
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G-Biosciences Calcein Release Assay (Cell Viability Assay)
Supplier: G-Biosciences
Calcein AM Cell Viability Assay can be easily adapted to various fluorescence setups such as microplate assays, fluorescence microscope and flow cytometry. The assay is useful for various studies such as cell viability, cell adhesion, chemotaxis, multidrug resistance, apoptosis and cytotoxicity.
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Amyloïde plaque kleuringsreagens, Amylo-Glo® RTD™
Supplier: Biosensis
Amylo-Glo RTD-reagens is ontworpen om amyloïde plaques in weefselcoupes te kleuren.
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Nrf2 transcription factor assay kit
Supplier: Cayman Chemical
Nrf2 Transcription Factor Assay kit is a 96-well assay for measurement of Nrf2.
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Red/green (FUN®1), ViaVac™
Supplier: Biotium
A fluorescent vitality dye for yeast, identical in structure to the dye FUN® 1. Living yeast cells show red fluorescent vacuolar tubules.
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Yeast vitality staining kit
Supplier: Biotium
A fluorescent vitality dye for yeast, identical in structure to the dye FUN® 1. Living yeast cells show red fluorescent vacuolar tubules.
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Live-or-Dye NucFix™ red staining kit
Supplier: Biotium
Live-or-Dye NucFix™ Red is a red fluorescent dye to covalently label dead cells, allowing cells with permeable plasma membranes to be detected by flow cytometry or fluorescence microscopy.
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Annexin V-FITC apoptosis detection kit II, Sigma-Aldrich®
Supplier: Merck Millipore (Oncogene)
Calbiochem® Annexin V-FITC Apoptosis Detection Kit II is a non-isotopic system that can be used to detect phosphatidylserine on the outer leaflet of the cell membrane of apoptotic cells using flow cytometry or fluorescence microscopy.
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BCA protein assay kit
Supplier: Thermo Fisher Scientific
BCA Protein Assay Kit can be used to measure very dilute protein concentrations in very small sample volumes. The kit accurately measures protein concentrations from 0,5 to 30 μg/ml in tube assays and 1 to 20 μg/ml in 96- or 384-well plate assays. It is used for studying protein:protein interactions, measuring column fractions after affinity chromatography, estimating percent recovery of membrane proteins from cell extracts and high-throughput screening of fusion proteins.
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Malachite green phosphate assay kit
Supplier: Abnova
Malachite Green Phosphate Assay Kit provides a fast, reproducible, and non-radioactive method for measuring inorganic free phosphate in aqueous solution.
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ATP assay kit
Supplier: Abnova
ATP Assay Kit is for the rapid bioluminescent determination of ATP in biological samples.
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Antioxidant assay kit
Supplier: Abnova
Antioxidant Assay Kit is a quantitative colorimetric determination of total antioxidant capacity of cellular samples.
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Pyruvate assay kit
Supplier: Abnova
Pyruvate Assay Kit is for quantitative colorimetric/fluorimetric pyruvate determination.
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Methylated DNA capture kit
Supplier: Abnova
Methylated DNA Capture Kit is used to enrich methylated DNA.
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Nitric oxide assay kit
Supplier: Abnova
Nitric Oxide Assay Kit for the quantitative colorimetric determination of nitric oxide in cell, food sample, plasma, serum, tissue and urine.