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Cellulaire assays

Cellulaire assays

Cellulaire assays zijn altijd een krachtig hulpmiddel geweest in het onderzoekslaboratorium. Ze zijn ongelooflijk veelzijdig en kunnen worden ontworpen om vrijwel elke cellulaire of biochemische functie te meten. Gevoelige detectie van fluorescerende probes helpt bij het evalueren van celsignalering en celdoodroutes, wat nuttig is voor de snelle kwantificatie van celviabiliteit. Cellulaire assays kunnen worden gebruikt om de effecten van verschillende behandelingen op de celviabiliteit te meten en om toxische effecten te evalueren die via uiteenlopende mechanismen worden gemedieerd.

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ExoBrite™ WGA EV Staining Kits

Supplier: Biotium

ExoBrite™ WGA EV staining kits were designed to overcome some of the challenges of EV detection, particularly in flow cytometry. ExoBrite™ WGA EV stains bind to molecules in the EV membrane for bright, specific staining, with little background.

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PPARα transcription factor assay kit

PPARα transcription factor assay kit

Supplier: Cayman Chemical

A 96-well assay for measurement of PPARα DNA binding activity.

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Cyclic-GMP fluorescent assay kits, CatchPoint™

Cyclic-GMP fluorescent assay kits, CatchPoint™

Supplier: Molecular Devices

CatchPoint™ cGMP Fluorescent Assay Kits measure cGMP levels via a competitive immunoassay format. The second messenger cyclic GMP (cGMP) modulates cellular processes, including smooth muscle relaxation, kidney function, and inflammation, by activating effectors such as protein kinases, cGMP-dependent phosphodiesterases, and cGMP-gated ion channels.

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Fatty Acid Uptake Assay Kit Abcam

Supplier: Abcam

Fatty Acid Uptake Assay Kit (ab287857) enables rapid determination of real-time fatty acid uptake kinetics in live cells expressing fatty acid transporter proteins.

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ViaFluor® 405 live cell microtubule stain 1000X in DMSO

Supplier: Biotium

Stain microtubules in live cells with blue fluorescence.

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2-NBDG Glucose Uptake Assay Kit Abcam

Supplier: Abcam

2-NBDG Glucose Uptake Assay Kit ab287845 uses the fluorescent glucose analog 2-NBDG to quantify the rate of cellular glucose uptake. Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

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Exosome Isolation and Analysis Kit - Flow Cytometry, Plasma (CD9 / CD81), Abcam

Supplier: Abcam

Exosome Isolation and Analysis Kit - Flow Cytometry, Plasma (CD9/CD81) (ab267479) is a simple immunobead assay for isolation/detection of exosome, using a bead-bound anti-CD9 capture antibody and a fluorochrome conjugated anti-CD81 detection antibody.

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dsDNA quantitation kits for the Qubit® fluorometer, AccuGreen™, high-sensitivity and broad-range

dsDNA quantitation kits for the Qubit® fluorometer, AccuGreen™, high-sensitivity and broad-range

Supplier: Biotium

AccuGreen™ kit is specific for dsDNA and can quantify DNA samples in the range of 0,1 to 10 ng/μl.

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Annexin V CF® dye conjugates, azide-free, lyophilised

Supplier: Biotium

Preservative-free Annexin V conjugates are compatible with detection of apoptotic cells in live cell imaging experiments, fluorescence microscopy, or flow cytometry.

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Fluorescerende zenuwterminale probes en kleuringen

Fluorescerende zenuwterminale probes en kleuringen

Supplier: Biotium

Nerve terminal probes are a series of fluorescent cationic styryl dyes developed to follow synaptic activity. These dyes have a lipophilic tail at one end and a hydrophilic cationically charged head group at the other end. The dyes vary in the number of carbons in the lipophilic tail (m) and the number of double bonds linking the two aromatic rings in the dye (n). AM dyes are similar to FM dyes, but contain an additional formaldehyde-fixable amino group in the cationic head.

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ALDetect™ Lipid Peroxidation Assay kit

Supplier: Enzo Life Sciences

The assay is designed to measure either MDA alone (in hydrochloric acid) or MDA in combination with 4-hydroxyalkenals (in methanesulfonic acid). It is based on the reaction of a chromogenic reagent, N-methyl-2-phenylindole with MDA and 4-hydroxyalkenals to yield a stable chromophore with maximal absorbance at 586 nm.

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Fluorescent calcium indicator, GFP-CERTIFIED® FLUOFORTE®

Supplier: Enzo Life Sciences

GFP-CERTIFIED® FLUOFORTE® is a fluorescent calcium indicator relative to other red fluorescent dyes.

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Cell proliferation assay kit, WST-1

Supplier: Cayman Chemical

WST-1 Proliferation Assay Kit provides an easy to use tool for studying the induction and inhibition of cell proliferation in any in vitro model. The assay is based on the reduction of tetrazolium salt WST-1 to soluble formazan by electron transport across the plasma membrane of dividing cells. This kit will also allow investigators to screen drug candidates involved in regulation of cell cycle.

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Potassium assay kits, FLIPR®

Potassium assay kits, FLIPR®

Supplier: Molecular Devices

The FLIPR® Potassium Assay Kit measures functional activity of ligand- and voltage-gated potassium channels. The homogeneous, no-wash assay protocol provides a large signal window and high Z' values.

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Reagents for Color-de-Lys™ HDAC colorimetric activity assay kit

Supplier: Enzo Life Sciences

Cellular assay, Color-de-Lys™ developer concentrate, 300 µl

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ADAM17 fluorometric drug discovery kit

Supplier: Enzo Life Sciences

Designed to screen ADAM17 (TACE) inhibitors using a quenched fluorogenic peptide. The ADAM17 Fluorometric (also known as fluorimetric) Drug Discovery Kit is a complete assay system designed to screen ADAM17 (TACE) inhibitors using a quenched fluorogenic peptide: Mca-PLAQAV-Dpa-RSSSR-NH2. Mca fluorescence is quenched by the Dpa group until cleavage by proteases separates the two moieties. The assays are performed in a convenient 96-well microplate format. The kit is useful to screen inhibitors of ADAM17, a potential therapeutic target. The compound GM6001 is also included as a prototypic control inhibitor.

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