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Supplier: Biotium

Cytotoxic agent that binds and inhibits dihydrofolate reductase. Useful for studying antimetabolite resistance, spontaneous gene amplification, and drug binding. Available conjugated to fluorescein and CF® Dyes.

Supplier: Biotium

Deze kits voor het labelen van antilichamen bevatten amine-reactieve near-IR CF™-kleurstof en alle componenten die nodig zijn voor het labelen en zuiveren van antilichamen.

Supplier: Biotium

NIR CF® dye free acids are non-reactive molecules and cannot be used for labeling. They can be used as reference standards or controls for corresponding NIR CF® dye-conjugates such as antibodies, proteins, peptides etc. in cell-based and in vivo assays.

Supplier: Merck

DPX new is a synthetic non aqueous mounting medium for microscopy. In its formulation, the teratogenic plasticiizer dibutyl phthalate (DBP) has been avoided, which renders the composition safer for the environment and more user friendly than the conventionally used mounting agents such as DPX.

Supplier: SIGMA ALDRICH MICROSCOPY

Bouin's solution is an excellent fixative for preserving soft and delicate structures. It is prepared with saturated picric acid, formaldehyde and acetic acid.

Supplier: SIGMA ALDRICH MICROSCOPY

Fluoroshield™ with DAPI is an aqueous mounting medium for preserving fluorescence of tissue and cell smears.

Supplier: SIGMA ALDRICH MICROSCOPY

Fluoromount™ Aqueous Mounting Medium for use with fluorescent dye-stained tissue.

Supplier: SIGMA ALDRICH MICROSCOPY

DPX Mountant for histology has been used as a slide mountant to fix sections.

Supplier: SIGMA ALDRICH MICROSCOPY

This is a mounting medium (contains 45% acrylic resin and 55% xylenes) used in microscopy. It is an adhesive and specimen preservative that can be used manually and in automated coverslipping equipment. Special purpose adhesive and sealant for use with glass, non plastic and other non-reactive materials.

Supplier: SIGMA ALDRICH MICROSCOPY

Differentiation solution comprises acidified ethanol or isopropanol that is used to decolourise hematoxylin stain. The duration of exposure to the solution determines the degree of decolourisation. Differentiation is a crucial step in hematoxylin-eosin staining to achieve the proper colour contrast necessary for visualisation, by selectively removing excess hematoxylin from the tissue section and leaving behind the desired staining intensity.

Supplier: SIGMA ALDRICH MICROSCOPY

D.P.X mounting medium is a mixture of distyrene (polystyrene), a plasticizer (tricresyl phosphate), and xylene. It facilitates the protection of stains and can dry quickly.

Supplier: VWR Chemicals

VividPro™ is a generic fluorescent stain for protein gel electrophoresis that can be used to visualize proteins.

Supplier: SIGMA ALDRICH MICROSCOPY

ImmunoHistoMount™ is a permanent aqueous mounting medium designed for tissue sections and cell smears with peroxidase and alkaline phosphatase chromogens that cannot be dehydrated with organic solvents.

Supplier: SIGMA ALDRICH MICROSCOPY

PolyFreeze is a water-soluble support matrix and a form of embedding medium for frozen sectioning. PolyFreeze sections flawlessly at routine frozen specimen thickness of 5-6 μ and leaves no slide residue. It provides for -20 ºC cryostat sectioning in rapid set embedment. This medium freezes quickly supporting the tissue for sectioning at 3 mm and up with no cracking of the matrix at temperatures from -8 °C to -25 °C.

Supplier: Merck

De gedefinieerde hardheid en uniformiteit van het te snijden materiaal zijn essentieel voor een consistente snijdkwaliteit. Aan deze vereisten kan worden voldaan met allerlei geschikte stoffen, zoals paraffines, harsen, gelatine en celloïdine.

Supplier: Merck

Used to increase lens effectiveness

Supplier: SIGMA ALDRICH MICROSCOPY

Immersion oil is used for high resolution (1000X) light microscopy work under oil immersion objective lens.

Supplier: SIGMA ALDRICH MICROSCOPY

Fluoroshield™ with 1,4-Diazabicyclo[2.2.2]octane is an aqueous mounting medium for preserving fluorescence of tissue and cell smears.

Supplier: Merck

Entellan® New is een nieuw montagemedium voor dekglaasjes en is vooral geschikt voor commerciële automatische inbouwinstrumenten die werken met dekglaasjes. De viscositeit varieert tussen 250 en 600 mPas, 1,00869 heeft een klein viscositeitsbereik. Dit nauwe viscositeitsbereik verbetert en versnelt de toepassing, omdat noch de druppelsnelheid noch het volume moeten worden gereset na de eerste ronde met een nieuwe fles Entellan® New.

Supplier: Merck

Watervrije montagemedia, zoals Neo-Mount®, moeten worden gebruikt voor permanente montage en langdurige opslag van monsters. Objectglaasjes die in pathologische laboratoria worden verwerkt, moeten tot 10 jaar worden opgeslagen, waarbij de kleuringsresultaten stabiel moeten blijven en verkleuring niet acceptabel is. Neo-Mount®, watervrij montagemedium voor microscopie, is bijzonder kleurvast en wordt vervaardigd van oplosmiddelen op basis van mengsels van alifatische koolwaterstoffen. Neo-Mount® bevat een aromavrije vervanging voor xyleen en mag dus alleen worden gecombineerd met Neo-Clear®. Xyleen moet worden vermeden in de opbouwstap, aangezien dit de objectglaasjes vertroebelt en streept. Als er bovendien gedurende ongeveer 1 minuut gedehydrateerde objectglaasjes op filterpapier vóór montage worden geplaatst, kan alle overtollige Neo-Clear® worden omzeild omdat er luchtbellen onder het dekglaasje kunnen worden gevormd. Er moet aan dezelfde voorwaarde worden voldaan bij de montage van monsters met machines die dekglaasjes gebruiken; in dit gebied kan NEO-CLEAR® het efficiëntst worden verwijderd door incubatie van de objectglaasjes gedurende één minuut in een leeg rek voor objectglaasjes.

Supplier: Merck

Aquatex is used for e.g. LEUCOGNOST stained specimens. For preservation purposes and subsequent optimal microscopy, the stained sections are normally mounted using a suitable mounting agent and covered with a cover glass.

Supplier: SIGMA ALDRICH MICROSCOPY

For use with Osteo-bed bone embedding kit. Solvent for removal of plastic from sections prior to rehydration and staining. Most histochemical and immunohistochemistry staining protocols require the removal of methyl methacrylate (MMA) and rehydration of mounted sections for improved stain penetration and greater contrast.

Supplier: SIGMA ALDRICH MICROSCOPY

The Epoxy Embedding Medium Kit is a very widely used embedding medium for electron microscopy. The embedding formulation originally published by Luft (1961) is excellent for both plant and animal tissues. Due to the low viscosity of the resin, it penetrates the tissue specimen faster than Araldite and other polymers. It hardens easily and uniformly at low temperatures with the addition of dodecenylsuccinic anhydride (DDSA), methyl nadic anhydride (MNA), and the accelerator 2,4,6-tris(dimethylaminomethyl)phenol (DMP-30). Slight shrinkage may occur during curing. This kit is useful for embedding a variety of tissues as a wide range of hardness can be obtained with this resin to suit a specific tissue type by using two different anhydride curing agents (DDSA and MNA).

Supplier: SIGMA ALDRICH MICROSCOPY

CC/mount is an aqueous based permanent mounting medium with a very high refractive index, which when applied to the stained tissue sections can store the tissue specimens permanently without the fading of the chromogens. Because of the superior refractive index of CC/mount, tissues mounted in this medium look like specimens mounted with organic based mounting media. No coverslipping is required with CC/mount. However, if coverslipping is required, dried CC/mount can be post mounted by using an organic based mounting medium. CC/mount permits the long-term storage of antigens localised using organic insoluble chromogen substrates, including AEC, DAB, Fast Red, BCIP/NBT, BCIP/INT and fluorescent dyes like FITC and phycobiliproteins. The high pH of CC/mount ensures increased stability of fluorescence intensity.

Supplier: Merck

Neo-Clear ® is een niet-gevaarlijke alternatief voor xyleen. Het is vrijwel reukloos, compatibel met de meeste oplosmiddelen met een hoge zuiverheid en wordt gekenmerkt door een lagere verdampingssnelheid dan xyleen, waardoor het risico voor de gezondheid kleiner is.

Supplier: SIGMA ALDRICH MICROSCOPY

Immersion oil has been used in epifluorescent microscopy and cell identification.

Supplier: SIGMA ALDRICH MICROSCOPY

Spurr resin is a hydrophobic resin. It is less viscous than epon mixtures and is easier to dispense. The low viscosity of the resin contributes to its penetration in tissue, mineral and dense structures. A low viscosity of 60 cps also allows easy penetration into a variety of difficult materials.

Supplier: SIGMA ALDRICH MICROSCOPY

HistoChoice® is a patented, complex mixture of agents that allow tissues to retain their structure, antigenic sites and nucleic acid sites. This formulation in non-citrus, contains no mercury, formaldehyde or glutaraldehyde.

Supplier: SIGMA ALDRICH MICROSCOPY

to be used with SIAL 78711 RenLam M-1 (Epoxy Embedding Medium)

Supplier: SIGMA ALDRICH MICROSCOPY

Fluoroshield™ is an aqueous mounting medium for preserving fluorescence of tissue and cell smears.