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Protein linking and labelling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colours, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labelling follows a simple protocol of reaction, separation, and then use.
Wheat Germ Agglutinin, XFD532 Labelled *XFD532 Same Structure to Alexa Fluor® 532*
Supplier: AAT Bioquest
XFD532, manufactured by AAT Bioquest, has an identical chemical structure to Alexa Fluor® 532, a trademark of ThermoFisher.
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Wheat Germ Agglutinin, XFD555 Labelled *XFD555 Same Structure to Alexa Fluor® 555*
Supplier: AAT Bioquest
XFD555, manufactured by AAT Bioquest, has an identical chemical structure to Alexa Fluor® 555, a trademark of ThermoFisher.
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Buccutite™ Rapid trFluor™ D2 Acceptor Antibody Labelling Kit
Supplier: AAT Bioquest
Microscale optimised for labelling 100 µg antibody per reaction. Many biological compounds present in cells, serum, or other biological fluids are naturally fluorescent, and thus the use of conventional, prompt fluorophores leads to serious limitations in assay sensitivity due to the high background caused by the autofluorescence of the biological molecules to be assayed.
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ReadiLink™ Rapid XFD555 Antibody Labelling Kit
Supplier: AAT Bioquest
ReadiLink™ Rapid antibody labelling kits, designed for production scale, provide a convenient and efficient method for labelling large volumes of antibodies with XFD dyes (equivalent to Alexa Fluor®).
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ReadiLink™ Rapid XFD647 Antibody Labelling Kit
Supplier: AAT Bioquest
ReadiLink™ Rapid antibody labelling kits, designed for production scale, provide a convenient and efficient method for labelling large volumes of antibodies with XFD dyes (equivalent to Alexa Fluor®).
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ReadiLink™ Rapid iFluor® 488 Antibody Labelling Kit
Supplier: AAT Bioquest
ReadiLink™ Rapid antibody labelling kits, designed for production scale, provide a convenient and efficient method for labelling large volumes of antibodies with our superior iFluor® dyes.
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Buccutite™ Streptavidin Antibody Conjugation Kit
Supplier: AAT Bioquest
Buccutite™ Streptavidin antibody conjugation kits are optimised for labelling either 25 µg, 100 µg or 1 mg of protein.
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HIS Lite™ Cy5 Bis NTA Chelator
Supplier: AAT Bioquest
Cy5 Bis NTA-Ni Chelator can be applied either to an SDS-PAGE gel or Western blot membrane for fluorescence imaging or used in living cells in combination with the addition of a heavy metal ion such as Ni²⁺.
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ReadiLink™ Rapid trFluor™ Tb Antibody Labelling Kit
Supplier: AAT Bioquest
Microscale optimised for labelling 50 µg antibody per reaction. Many biological compounds present in cells, serum or other biological fluids are naturally fluorescent, and thus the use of conventional, prompt fluorophores leads to serious limitations in assay sensitivity due to the high background caused by the autofluorescence of the biological molecules to be assayed.
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ReadiLink™ iFluor® 647 Oligo and ssDNA Labeling Kit
Supplier: AAT Bioquest
The kit is optimised for efficient labelling and contains all the essential reagents required for efficient labelling of ssDNA or oligos.
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ReadiLink™ Cy5 Oligo and ssDNA Labeling Kit
Supplier: AAT Bioquest
The kit is optimised for efficient labelling and contains all the essential reagents required for efficient labelling of ssDNA or oligos.
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DY547 CEP (DyLight 547 Phosphoramidite)
Supplier: AAT Bioquest
DY547 CEP (DyLight 547 phosphoramidite) is an excellent replacement to the commonly used Cy3 phosphoramidite.
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HEX-dT Phosphoramidite
Supplier: AAT Bioquest
HEX-dT phosphoramidite provides a valuable tool to add the HEX tag to a desired location of an oligo sequence.
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Buccutite™ FOL, NHS ester (2 µmoles)
Supplier: AAT Bioquest
Our Buccutite™crosslinking technology provides the most convenient and effective crosslinking method to link two biomolecules with a high conjugation yield.
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Buccutite™ ALP (Alkaline Phosphatase) Antibody Conjugation Kits (2 Labelings)
Supplier: AAT Bioquest
Protein-protein conjugations are commonly performed with a bifunctional linker (such as SMCC) having different reactivity at each end for linking two different proteins.
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ReadiLink™ Rapid iFluor® Antibody Labeling Kit "Microscale Optimized for Labeling 50 µg Antibody Per Reaction" (2 Labelings)
Supplier: AAT Bioquest
AAT Bioquest's iFluor® dyes are optimised for labelling proteins, in particular, antibodies.
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ReadiLink™ Rapid iFluor® 680 Antibody Labelling Kit 'Microscale Optimised for Labelling 50 µg Antibody Per Reaction' (2 Labellings)
Supplier: AAT Bioquest
AAT Bioquest's iFluor® dyes are optimised for labelling proteins, in particular, antibodies.
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ReadiLink™ Rapid mFluor™ Green 620 Antibody Labeling Kit "Microscale Optimized for Labeling 50 µg Antibody Per Reaction" (2 Labelings)
Supplier: AAT Bioquest
AAT Bioquest's mFluor™ dyes are developed for flow cytometry-focused applications.
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Buccutite™ Rapid Protein Crosslinking Kit *Microscale Optimized for Crosslinking 100 ug Antibody Per Reaction* (2 Labelings)
Supplier: AAT Bioquest
Protein-protein conjugations are commonly performed with a bifunctional linker such as SMCC.
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Buccutite™ Rapid PE-Cy5.5 Tandem Antibody Labeling Kits (2 Labellings)
Supplier: AAT Bioquest
PE-Cy5.5 is a popular colour used in flow cytometry.
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Buccutite™ Rapid PE-Cy7 Tandem Antibody Labelling Kits (2 Labellings)
Supplier: AAT Bioquest
PE-Cy7 is a popular colour used in flow cytometry.
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ReadiLink™ xtra Rapid Cy3 Antibody Labeling Kit *BSA-Compatible* (2 Labelings)
Supplier: AAT Bioquest
ReadiLink™ xtra rapid antibody labelling kits require essentially only 2 simple mixing steps without a column purification needed.
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PSA™ iFluor® 594 Imaging Kit with Goat Anti-Mouse IgG
Supplier: AAT Bioquest
iFluor® 594 PSA kit is a much superior replacement for Alexa Fluor 594 tyramide-based kit or other spectrally similar fluorescent tyramide or TSA kits.
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Buccutite™ FOL scavenger (2 µmoles)
Supplier: AAT Bioquest
Our Buccutite™crosslinking technology provides the most convenient and effective crosslinking method to link two biomolecules with a high conjugation yield.
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Fluor phosphine reagents, DyLight™, Pierce™
Supplier: Thermo Fisher Scientific
DyLight™ Fluor phosphine reagents are phosphine-activated fluorescent dyes for specific labeling and detection of azide-tagged molecules, which enables use of fluorescence imaging in metabolic labeling strategies.
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Carbohydrate reactive biotinylation reagents, EZ-Link™
Supplier: Thermo Fisher Scientific
A common target for protein modification is the carbohydrate portion of glycoproteins, which can be reacted with hydrazide derivatives of biotin. Oxidative treatment of glycoproteins using 10 mM periodate is used to generate reactive aldehydes from the cis-diols of a variety of carbohydrate moieties.
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Avidin, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ Avidin is used in biotin-based detection methods, including Western blotting or ELISA detection with appropriate enzyme substrates. It is a purified protein from egg whites and it is stable over wide range of pH and temperature.
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Dye Labeling Kit (5/6) TAMRA-SE (Rhodamine), HOOK™
Supplier: G-Biosciences
The HOOK™ Dye Labeling Kit (TAMRA-SE (5-(and-6)- Carboxytetramethylrhodamine succinimidyl ester, mixed isomers) is based on tetramethylrhodamine, one of the most common fluorophores used in the labelling of peptides, proteins, nucleic acids and nucleotides. It absorbs green visible light at 546 nm and emits an orange-red visible light at a maximum emission of 575 nm.
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Carboxyl reactive biotin reagents, HOOK™
Supplier: G-Biosciences
HOOK™ Biotin-PEG₂-Amine, and its long chain form, HOOK™ Biotin-PEG₃-Amine, are carboxyl reactive biotinylation reagents. These agents contain terminal amines and react with carboxyl groups found at the carboxyl termini, aspartate, and glutamate side chains. The reaction is mediated by a water-soluble carbodiimide (EDC). The carbodiimide activates the carboxyl group and reacts with the amines (-NH₂) on the biotinylation agent to form an amide bond. This reaction is rapid and takes just a few minutes to complete. Under these conditions, hydrazide-derivatives of biotin reagents may also react with the carboxyls.
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Photoreactive biotin reagents, HOOK™ Psoralen-PEO-Biotin
Supplier: G-Biosciences
Photoreactive agents, on exposure to ultraviolet light, become active and bind non specifically with neighbouring molecules. These reagents are suitable for labelling molecules that do not contain easily reactable functional groups. Photoreactive reagent HOOK™ Psoralen-PEO-Biotin reacts with and labels nucleic acids and protein molecules. When reacted with nucleic acids, it crosslinks with pyrimidine bases. Crosslinking does not interfere with hybridisation applications.