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Protein linking and labelling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colours, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labelling follows a simple protocol of reaction, separation, and then use.
Antibody labelling kits, Mix-n-Stain™
Supplier: Biotium
Mix-n-Stain™ antibody labelling kits utilise a technology that allows rapid antibody labelling without a purification step.
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Affibody® Imaging Agent, Negative Control
Supplier: Abcam
Product to be used as a negative control for ErbB 2 Affibody® Molecule Imaging Agent.
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HRP-conjugate stabiliser, SeramunStab® Stplus
Supplier: Apollo Scientific
Liquid product colourless to slightly yellow or greenish, odourless, pH 7,3 to 7,5. Stabiliser for conjugates with monomeric HRP.
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Biotin conjugation estimation, HOOK™ BiotinQuant
Supplier: G-Biosciences
HOOK™ BiotinQuant is designed for the estimation of biotin conjugation. It measures biotin using HABA [4’-hydroxyazobenzene-2-carboxylic acid] dye. HABA binds with avidin at the biotin-binding site.
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Lightning-Link® Rapid DyLight 450 Antibody Labeling Kits
Supplier: Novus Biologicals
Lightning-link rapid is an innovative technology that enables direct labeling of proteins, peptides or other biomolecules for use in R&D applications, drug discovery and the development of diagnostic kits.
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Single-round RNA amplification and biotin labeling system
Supplier: Enzo Life Sciences
The Single-Round RNA Amplification and Biotin Labeling System provides an optimized protocol and reagents for the production of biotin-labeled antisense RNA (aRNA) from total cellular RNA samples in less than 24 hours for array analysis.
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Red 580 dUTP
Supplier: Enzo Life Sciences
Red 580 [5-ROX] dUTP can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labelled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, random prime labelling, cDNA labelling and 3’-end labelling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Red 580 dUTP can also be used for multicolor fluorescence labelling.
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Aqua 431 dUTP
Supplier: Enzo Life Sciences
Aqua 431 [DEAC] dUTP is a coumarin derivative that can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labelled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, random prime labelling, cDNA labelling and 3’-end labelling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Aqua 431 dUTP can also be used for multicolor fluorescence labelling.
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Nuclear-ID™ Blue/Green cell viability reagent
Supplier: Enzo Life Sciences
Nucleus dye
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Nuclear-ID™ red/green cell viability reagent
Supplier: Enzo Life Sciences
Cell viability dye
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Chemiluminescent labelling kit
Supplier: Enzo Life Sciences
A complete kit for the labeling of antibodies, proteins, peptides, and nucleic acids with Acridinium NHS Ester.
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IgG Biotinylation kits, HOOK™
Supplier: G-Biosciences
HOOK™ IgG biotinylation kits are designed for the biotinylation of IgG molecules by immobilising the IgG molecules on a solid support. Nickel chelating resin allows for the rapid removal of uncoupled biotin and therefore eliminates the need for further dialysis or desalting of the biotinylated antibody.
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Lipophilic carbocyanine dyes and CellBrite cytoplasmic membrane labelling kits
Supplier: Biotium
Carbocyanine dyes with long hydrocarbon chains have low fluorescence in aqueous phase, but become highly fluorescent upon binding to lipid bilayers, making this class of dyes ideal for staining cell membranes. Carbocyanine dye membrane staining is very stable, with minimal dye transfer between cells. Dilinoleyl carbocynaine dyes have much faster lateral diffusion rates than regular carbocyanine dyes, which facilitates labelling of neurons in tissues.
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Dye tyramide, CF®
Supplier: Biotium
Fluorescent CF® dye tyramides are used for tyramide signal amplification (TSA) for increasing immunofluorescence sensitivity in multicolour immunocytochemistry (ICC), immunohistochemistry (IHC), or in situ hybridization (ISH).
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Annexin V Near IR CF® dye conjugates
Supplier: Biotium
Near-IR Annexin V fluorescent conjugates can be used to detect apoptotic cells and is suitable for in vivo imaging.
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CDI amine reactive resin
Supplier: G-Biosciences
CDI amine reactive agarose consists of 6% cross-linked agarose activated with CDI (1,1’-carbonyl diimidazole) to form reactive imidazole carbamates.
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CF™ Dye lectin conjugates
Supplier: Biotium
A lectin labelled with our superior fluorescent CF® dyes is an effective and widely used cell surface stain for yeast, fungi, and mammalian cells.
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Biotin picolyl azide
Supplier: Biotium
Biotin picolyl azide reacts with alkyne to form 1,2,3-triazole by 1,3-dipolar Huisgen cycloaddition through the use of a much lower copper (I) concentration without sacrificing reaction efficiency.
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Orange 552 dUTP (lyophilised)
Supplier: Enzo Life Sciences
Orange 552 dUTP (lyophilised)
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Orange 552 dUTP
Supplier: Enzo Life Sciences
Orange 552 dUTP (5-TAMRA-dUTP) can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labeled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, cDNA labeling and 3’-end labeling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Orange 552 dUTP can also be used for multicolor fluorescence labeling.
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ORGANELLE-ID-RGB® III Assay kit
Supplier: Enzo Life Sciences
TheORGANELLE-ID-RGB® III assay kit contains Golgi apparatus-selective, endoplasmic reticulum-selective and nucleus-selective dyes suitable for live cell staining.
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Reactive biotin compounds
Supplier: Biotium
Biotium offers reactive biotin compounds with a variety of reactive functional groups.
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Phalloidine conjugaties
Supplier: Biotium
Phalloidin is a toxin isolated from the deadly Amanita phalloides mushroom. It is a bicyclic peptide that binds specifically to F-actin. Fluorescent conjugates of phalloidin are convenient tools to investigate the distribution of F-actin in fixed and permeabilised cells or tissue sections. One unit (U) of phallodin is sufficient to stain one sample (200 µl staining volume). A highly selective actin filament stain for fixed and permeabilised cells. Labeled phalloidins have similar affinity for both large and small filaments.
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Annexin V conjugates
Supplier: Biotium
Annexin V fluorescent conjugates can be used to detect apoptotic cells. Annexin V is available conjugated to a wide variety of dyes.
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Antibody labelling kits, Mix-n-Stain™ Biotin
Supplier: Biotium
Mix-n-Stain™ Biotin antibody labelling kits utilise a technology that allows rapid antibody labelling with biotin without a purification step.
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CF® Dye hydrazide
Supplier: Biotium
Fluorescent CF® Dye hydrazides are bright, extremely water-soluble, nontoxic molecules, useful as aldehyde-fixable cell tracers. They can also be used to label aldehyde or ketone groups of polysaccharides, carbohydrates or glycoproteins.
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Carboxyl coupling resin
Supplier: G-Biosciences
Carboxyl coupling resin consists of 6% cross-linked agarose with covalently linked diaminodipropylamine (DADPA) to generate a free primary amine at the end of a long spacer arm. Molecules, including proteins, peptides and nucleic acids, are covalently coupled to the free primary amines, and the stable columns are ideal for affinity purification of antibodies and other interacting partners.
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Amine reactive biotin reagents, HOOK™
Supplier: G-Biosciences
HOOK™ NHS-Biotin, 50 mg
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CF™ Dyes Cholera Toxin Subunit B, CF™ Dye Conjugates
Supplier: Biotium
Cholera toxin subunit B conjugated to Biotium's next-generation fluorescent CF™ dyes.
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Tyramide amplification kit with HRP goat, anti-rabbit and CF® dye or biotin tyramide
Supplier: Biotium
Kits for increasing immunofluorescence sensitivity using HRP-catalysed tyramide signal amplification with the choice of next-generation fluorescent CF® dyes or biotin.