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378 resultaten voor Koppelings- en labelingssystemen voor eiwitten

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Koppelings- en labelingssystemen voor eiwitten

Protein linking and labelling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colours, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labelling follows a simple protocol of reaction, separation, and then use.

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ZnAF-2 DA

Supplier: Enzo Life Sciences

Fluorescent probe for Zn²⁺. Cell permeable.

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Green 496 dUTP (lyophilized)

Green 496 dUTP (lyophilized)

Supplier: Enzo Life Sciences

Green 496 dUTP (lyophilised)

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Green 496 dUTP

Supplier: Enzo Life Sciences

Green 496 [5-Fluorescein] dUTP can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase.

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Aqua 431 dUTP (lyophilised)

Supplier: Enzo Life Sciences

Aqua 431 dUTP (lyophilised)

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Organelle-ID™ RGB reagent I

Supplier: Enzo Life Sciences

Mixture of fluorescent dyes for detection of lysosomes, mitochondria and nucleic acids

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Wheat germ agglutinin (WGA) conjugates

Wheat germ agglutinin (WGA) conjugates

Supplier: Biotium

A wheat germ agglutinin (WGA) lectin labelled with Biotium’s CF® Dyes or HRP. It is a carbohydrate-binding lectin that has high affinity for sialic acid and N-acetylglucosamine moieties of glycoproteins. As a result, WGA conjugates will label yeast bud scars and the cell membrane of gram-positive bacteria and mammalian cells.

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ExoBrite™ True EV Membrane Stains

Supplier: Biotium

Lipophilic membrane dyes designed specifically for EV research and the best choice for general pan-EV staining.

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Coelenterazine Substrates for Renilla Luciferase and Apoaequorin

Coelenterazine Substrates for Renilla Luciferase and Apoaequorin

Supplier: Biotium

Coelenterazine and its analogs are luminescent enzyme substrates for apoaequorin and Renilla luciferase.

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Antibody labelling kits, Mix-n-Stain™ FITC

Antibody labelling kits, Mix-n-Stain™ FITC

Supplier: Biotium

Mix-n-Stain™ FITC antibody labelling kits utilise a technology that allows rapid antibody labelling with green fluorescent dye (FITC) without a purification step.

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CF® Dye cAMP

Supplier: Biotium

Fluorescently labelled cAMP analogs that can be used to probe cAMP receptors.

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Tyramide amplification buffer plus

Tyramide amplification buffer plus

Supplier: Biotium

An optimized buffer with enhanced sensitivity for tyramide signal amplification of immunostaining in cells or tissue sections.

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R-Phycoerythrin (RPE)

R-Phycoerythrin (RPE)

Supplier: Columbia Biosciences

Many applications and instruments were developed specifically for R-phycoerythrin.

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CF™ dye streptavidin and monoclonal mouse anti-biotin conjugates

Supplier: Biotium

CF™ dyes are superior to other fluorescent dyes for protein labelling due to advantages in brightness, photostability and water solubility.

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HRP-conjugate stabiliser, SeramunStab® STA2

Supplier: Apollo Scientific

Liquid product red odourless pH-value 7,3 to 7,5 Boiling point 102 °C. Stabiliser for a broad range of HRP conjugates.

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Fluorescein-12-ddUTP

Fluorescein-12-ddUTP

Supplier: Enzo Life Sciences

Fluorescein-12-ddUTP can be used for 3’-end labeling of oligodeoxynucleotides using terminal transferase. It also serves as a substrate for E.coli DNA Polymerase I (holoenzyme and Klenow fragment), T4 and T7 DNA polymerases, Taq DNA polymerase and reverse transcriptase. Oligonucleotide probes labelled with fluorescein-12-ddUTP can be detected by direct fluorescence detection (green fluorescence) or by methods using horseradish peroxidase- or alkaline phosphatase-linked anti-fluorescein antibodies.

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Bio-16-ddUTP, tetralithium salt

Bio-16-ddUTP, tetralithium salt

Supplier: Enzo Life Sciences

Bio-16-ddUTP (Biotin-16-2’,3’-dideoxyuridine-5’-triphosphate) can be used for 3’-end labelling of single-stranded or double-stranded DNA. It is especially useful as a substrate for terminal transferase for the 3’-terminal addition of a single Bio-16-ddUMP. Biotin-labelled oligonucleotides produced by this reaction are suitable as hybridisation probes and nucleic acid array assays. It is also a substrate for Taq DNA polymerase and other DNA polymerases.

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Gold 550 dUTP

Supplier: Enzo Life Sciences

Gold 550 [Cyanine-3E] dUTP is a cyanine-3 derivative with enhanced spectral properties. This nucleotide analogue can replace TTP in reactions in which it serves as a substrate for E.coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase.

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Gold 525 dUTP (lyophilised)

Supplier: Enzo Life Sciences

Gold 525 dUTP (lyophilised)

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Organelle-ID™ RGB reagent II

Supplier: Enzo Life Sciences

Mixture of fluorescent dyes for detection of lysosomes, endoplasmic reticulum and nucleic acids

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CF® Dye Azide

CF® Dye Azide

Supplier: Biotium

CF® Dyes with an azide group. Can be used to label or detect alkyne, BCN or cyclooctyne-containing molecules by fluorescence spectroscopy, microscopy or flow cytometry following copper-catalyzed azide-alkyne cycloaddition.

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Thiol-reactive dyes and reagents

Thiol-reactive dyes and reagents

Supplier: Biotium

MTS, TS and maleimide, and other thiol-modifying reagents and reactive fluorescent dyes.

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CF®DI Aminooxy

Supplier: Biotium

CF®DI aminooxy dyes react with aldehyde/ketone groups on proteins or other biomolecules to form a stable oxime bond. The CF®DI dyes (CF®488DI, CF®555DI and CF®647DI) are designed to match each other for molecular weight and charge. The molecular weights of the same protein conjugated to different CF®DI dye colors are close to each other and the pl of the labeled proteins remains the same.

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ExoBrite™ EV Surface Stain Sampler Kit

Supplier: Biotium

The ExoBrite™ EV Surface Stain Sampler Kit, Green was developed to offer each of Biotium’s ExoBrite™ EV Surface Stains (CTB, WGA, and Annexin V) for assessing which stain offers the best coverage for the EV samples of interest.

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Bungarotoxins and fluorescent bungarotoxin conjugates

Bungarotoxins and fluorescent bungarotoxin conjugates

Supplier: Biotium

Bungarotoxins are venom toxins from the many-banded krait (Bungarus Multicinctus), which inhibit neurotransmission at the neuromuscular junction. Alpha-bungarotoxin is a potent inhibitor for the motor endplate acetylcholine receptor (Kd = 1 nM to 1 pM). Beta-bungarotoxin contains a small subunit that blocks voltage-gated K+ channels on the presynaptic membranes, and a large subunit that has phospholipase A2 activity. Fluorescent conjugates of alpha-bungarotoxin can be used to label acetylcholine receptors in skeletal muscle endplates.

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Streptavidin conjugates

Streptavidin conjugates

Supplier: Biotium

A high quality biotin-binding protein conjugated to Biotium's signature bright and photostable fluorescent CF® dyes, and a selection of other labels.

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CF® Dye BCN

CF® Dye BCN

Supplier: Biotium

CF® Dyes with a BCN group react with azides via a copper-free bioorthogonal reaction to label target molecules for surface staining of live cells or cell extracts. Membrane-permeant CF® Dye BCN conjugates for intracellular labelling reactions are also available.

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Carbohydrate coupling resin

Carbohydrate coupling resin

Supplier: G-Biosciences

Carbohydrate coupling resin is designed for the covalent immobilisation of carbohydrate containing molecules, including glycoproteins, to agarose beads. It is ideal for the coupling of polyclonal antibodies as it allows for the optimal orientation of the antibodies for affinity purification.

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CF™ dye picolyl azide

Supplier: Biotium

CF® dye picolyl azide reacts with alkyne to form 1,2,3-triazole by 1,3-dipolar Huisgen cycloaddition through the use of a much lower copper (I) concentration without sacrificing reaction efficiency. The selected CF® dyes are exceptionally bright and photostable, making them the perfect dye for fluorescence detection.

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Tyramide amplification kit with HRP goat, anti-mouse and CF® dye or biotin tyramide

Supplier: Biotium

Kits for increasing immunofluorescence sensitivity using HRP-catalysed tyramide signal amplification with the choice of next-generation fluorescent CF® dyes or biotin.

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Tyramide amplification kit with HRP streptavidin and CF® dye or biotin tyramide

Supplier: Biotium

Kits for increasing immunofluorescence sensitivity using HRP-catalysed tyramide signal amplification with the choice of next-generation fluorescent CF® dyes or biotin.

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