Buffers
Buffers zijn bestand tegen pH-veranderingen en beschermen monsters wanneer zuren of basen worden toegevoegd. Zowel in poeder- als vloeibare vorm zijn ze geschikt voor het efficiënt volgen van eiwitten tijdens elektroforetische isolatie en scheiding. De zoute buffers kunnen ook worden gebruikt om pH-meters te kalibreren en te standaardiseren voor nauwkeurige metingen. Blokkerende stoffen voorkomen de hechting van antilichamen aan membraanvrije plaatsen die een storende achtergrond zouden veroorzaken. De buffers zijn verkrijgbaar in verschillende concentraties, voor elke toepassing, en kunnen vloeibaar of gedehydrateerd zijn.
SSC buffer, 20X concentrate, pH 7,0
Supplier: Rockland Immunochemicals
Saline-sodium citrate (SSC) buffer is most commonly used as a buffer for the hybridisation process and washing in experiments where spcies of nucleic or ribonucleic acid are to be matched to complimentary sequences (such as Southern and Nouthern blotting, in situ hybridisation, or DNA microarrays). SSC is used to help control the stringency of hybridisation in these steps.
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RIPA Buffer, Abcam
Supplier: Abcam
A ready-to-use buffer useful for lysing cells and tissues for radioimmunoprecipitation assay.
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Tissue Clearing Permeabilization Buffer - hydrophobic, Abcam
Supplier: Abcam
Tissue Clearing Permeabilization Buffer ab243307 is part of our range of tissue clearing reagents and products that enable you to set up tissue clearing easily and quickly.
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MMP-1 colorimetric assay buffer
Supplier: Enzo Life Sciences
MMP-1 colorimetric assay buffer
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Proteometer-UFT buffer packets
Supplier: NOVILYTIC
3 sealed buffer packets specifically formulated for use with Proteometer-UFT kit.
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Protein Block, Abcam
Supplier: Abcam
Protein block is a serum-free blocking buffer designed to reduce non-specific background staining in IHC, ELISA, and western blot (WB). - Cited in over 90 publications - No need to match species for secondary antibodies, reducing animal serum handling - Ready-to-use – no mixing or dilution required
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GET buffer
Supplier: Rockland Immunochemicals
GET buffer (50 mM Glucose, 10 mM EDTA, 25 mM Tris HCl, pH 8,0) is used in effective plasmid miniprep.
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Buffer solution pH 5, Acetate Buffer
Supplier: Thermo Fisher Scientific
Acetate buffer, Contains 0.7 ml glacial acetic acid and 0.6 g potassium hydroxide per litre., pH-waarde: 4,98 - 5,02 (20 °C)
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HeLa Nuclear Extract, Abcam
Supplier: Abcam
Nuclear extract is prepared from HeLa cells, which contains a variety of DNA-binding proteins, transcription factors, and other nuclear proteins.
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TE Buffer (1X) pH 8,0 for Molecular Biology
Supplier: Apollo Scientific
Cloning Buffer, TE buffer (1X)
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Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l)
Supplier: PanReac AppliChem
Cloning Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l)
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Wash buffer concentrate
Supplier: Enzo Life Sciences
Liquid. TRIS buffered saline containing detergents.
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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer)
Supplier: Rockland Immunochemicals
Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)
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Buffer, TBE solution 5X concentrate (TRIS-borate-EDTA buffer)
Supplier: Biotium
Running Buffer, TBE buffer solution 5X concentrate (TRIS-borate-EDTA buffer)
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