Buffers
Buffers zijn bestand tegen pH-veranderingen en beschermen monsters wanneer zuren of basen worden toegevoegd. Zowel in poeder- als vloeibare vorm zijn ze geschikt voor het efficiënt volgen van eiwitten tijdens elektroforetische isolatie en scheiding. De zoute buffers kunnen ook worden gebruikt om pH-meters te kalibreren en te standaardiseren voor nauwkeurige metingen. Blokkerende stoffen voorkomen de hechting van antilichamen aan membraanvrije plaatsen die een storende achtergrond zouden veroorzaken. De buffers zijn verkrijgbaar in verschillende concentraties, voor elke toepassing, en kunnen vloeibaar of gedehydrateerd zijn.
VWR® Ultra Pure Grade, Sm buffer
Supplier: VWR Chemicals
Cloning Buffer, SM BUFFER
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VWR® Sodium Acetate Buffer 3,0 M Solution, pH 5,2
Supplier: VWR Chemicals
With diethyl pyrocarbonate (DEPC).
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Fosfaatgebufferde zoutoplossing (Phosphate Buffered Saline, PBS), hoog fosfaatgehalte
Supplier: VWR Chemicals
Geïrradieerde oplossing, pH 7,2, voor het wassen van blots.
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VWR®, Tris-borate-EDTA (TBE) buffer
Supplier: VWR Chemicals
TBE is optimised for DNA electrophoresis techniques using both acrylamide and agarose. Buffers are available as liquid concentrate or Ready-Pack™ (a foil pouch containing sufficient material to prepare 1 litre of 10X concentrate).
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VWR®, Phosphate Buffered Saline (PBS), pH 7,4
Supplier: VWR Chemicals
Phosphate Buffered Saline (PBS) is a buffer solution containing sodium chloride, sodium phosphate and potassium chloride. PBS helps to maintain a constant pH, is isotonic and non toxic to cells, and provide cells with water and essential inorganic ions. This formulation does not contain calcium and magnesium.
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VWR® UltraPure, PBS and TBS wash buffers
Supplier: VWR Chemicals
Wash buffers ideal for use in applications such as Western blotting and ELISA. Prevents high background by effectively removing unbound reagents.
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VWR® AVS TITRINORM Buffer Solution, pH 9 (at 20 °C), Borate free
Supplier: VWR Chemicals
Solution prepared from AnalaR® NORMAPUR® grade analytical reagents.
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VWR® UltraPure, Buffer, concentrate (phosphate buffered saline)
Supplier: VWR Chemicals
Washing buffer, PBS buffer solution 10X concentrate (phosphate buffered saline)
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J.T.Baker®, Sheath fluid, 3000/3500
Supplier: Avantor
Sheath fluid 3000/3500, J.T.Baker®
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VWR® Buffer, MOPS-SDS, 20X, UltraPure Grade
Supplier: VWR Chemicals
Useful for high resolution of proteins on neutral pH SDS-PAGE gels.
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VWR®, Buffers, Sodium acetate
Supplier: VWR Chemicals
Sodium acetate buffer C, VWR®
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VWR®, Phosphate buffered saline (PBS) 1X, for biotechnology
Supplier: VWR Chemicals
137 mM sodium chloride, 2,7 mM potassium chloride, 12 mM phosphate buffer
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VWR® PBS/Tween® 20, 20X, Blot Wash Buffer, UltraPure Grade
Supplier: VWR Chemicals
PBS Tween® 20 blot wash buffer is a 20X concentration with 1% Tween® 20.
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J.T.Baker® DILUT-IT™, Potassium Phosphate, Dissolution Media Concentrate
Supplier: Avantor
DILUT-IT is a ready-to-use dissolution media concentrate, which is pre-measured and only need to be diluted with purified water.
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VWR® Buffer, Ammonium Chloride, pH 10, Reag. Ph.Eur.
Supplier: VWR Chemicals
The reagent is specially intended for use in testing Ph Eur and monographs. This solution was produced according 4.1.1 of Ph Eur specifications using Ph Eur reagent quality components and water meeting the Ph Eur monograph requirement for purified water.
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VWR® Tris Buffer Solution, pH 7,4 (0,1 mol/L) for Biotechnology
Supplier: VWR Chemicals
Cell culture buffer, TRIS buffer solution pH 7,4 (0,1 mol/l)
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